PMID- 32373945
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20210104
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 24
IP  - 8
DP  - 2020 Apr
TI  - LncRNA H19 inhibitor represses synovial cell proliferation and apoptosis in rats 
      with rheumatoid arthritis via Notch signaling pathway.
PG  - 4088-4094
LID - 20985 [pii]
LID - 10.26355/eurrev_202004_20985 [doi]
AB  - OBJECTIVE: To study the roles and underlying mechanisms of long non-coding 
      ribonucleic acid (lncRNA) H19 in the synovial cell proliferation and apoptosis in 
      rats with rheumatoid arthritis (RA). MATERIALS AND METHODS: A total of 30 
      Sprague-Dawley rats were randomly divided into Control group and Model group. The 
      rat model of RA was induced by using type II collagen in Model group. The primary 
      synovial cells were isolated from the synovial tissues of the rats and were 
      assigned into Control group, Model group, and lncRNA H19 inhibitor intervention 
      group. 5-Ethynyl-2'-deoxyuridine (EdU) staining was applied to detect cell 
      proliferation in each group. Terminal deoxynucleotidyl transferase-mediated dUTP 
      nick end labeling (TUNEL) staining was employed to determine the cell apoptosis 
      in each group. Western blotting assay was adopted to measure the expression 
      levels of Notch1 and hairy/enhancer of split-1 (Hes1) in each group of cells. 
      RESULTS: The RA score of the Model group was higher than that of the Control 
      group. Compared to the Control group, the expression of lncRNA H19, Notch, and 
      Hes1 of the synovial cells in the Model group were significantly elevated. 
      Besides, the cell proliferation rate of the Model was also increased, while the 
      cell apoptosis rate was decreased compared with those in the Control group. 
      Moreover, in comparison with Model group, lncRNA H19 inhibitor intervention group 
      exhibited a lowered lncRNA H19 level, remarkably reduced cell proliferation rate 
      and protein levels of Notch1 and Hes1, as well as notably raised cell apoptosis 
      rate. CONCLUSIONS: Our results indicated that lncRNA H19 inhibitor could repress 
      the proliferation and promote the apoptosis of synovial cells in RA rats, which 
      might be attributed to the inhibition of the Notch signaling pathway.
FAU - Zhi, L-Q
AU  - Zhi LQ
AD  - Department of Joint Surgery, Xi'an Honghui Hospital, Xi'an Jiaotong University, 
      Xi'an China. 198746wangxi@163.com.
FAU - Zhong, Q
AU  - Zhong Q
FAU - Ma, J-B
AU  - Ma JB
FAU - Xiao, L
AU  - Xiao L
FAU - Yao, S-X
AU  - Yao SX
FAU - Wang, X
AU  - Wang X
LA  - eng
PT  - Journal Article
PT  - Retracted Publication
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
SB  - IM
RIN - Eur Rev Med Pharmacol Sci. 2020 Aug;24(15):7921. PMID: 32767314
EDAT- 2020/05/07 06:00
MHDA- 2020/05/07 06:01
CRDT- 2020/05/07 06:00
PHST- 2020/05/07 06:00 [entrez]
PHST- 2020/05/07 06:00 [pubmed]
PHST- 2020/05/07 06:01 [medline]
AID - 20985 [pii]
AID - 10.26355/eurrev_202004_20985 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2020 Apr;24(8):4088-4094. doi: 
      10.26355/eurrev_202004_20985.