PMID- 32376549
OWN - NLM
STAT- MEDLINE
DCOM- 20200629
LR  - 20200629
IS  - 1673-4254 (Print)
IS  - 2663-0842 (Electronic)
IS  - 1673-4254 (Linking)
VI  - 40
IP  - 1
DP  - 2020 Jan 30
TI  - [Vitamin D receptor and monocyte chemoattractant protein-1 expressions in 
      peripheral blood mononuclear cells in patients with systemic lupus 
      erythemaotsus].
PG  - 99-103
LID - 10.12122/j.issn.1673-4254.2020.01.16 [doi]
AB  - OBJECTIVE: To detect the expressions of vitamin D receptor (VDR) in peripheral 
      blood monocytes (PBMCs) and its association with monocyte chemoattractant 
      protein-1 (MCP-1) in patients with systemic lupus erythemaotsus (SLE). METHODS: 
      We examined the expressions of VDR and MCP-1 mRNAs in the PBMCs in 60 SLE 
      patients and 28 healthy individuals using real- time quantitative PCR. We also 
      detected the expression of VDR protein in the PBMCs using Western blotting and 
      peripheral blood MCP-1 level using ELISA for these participants. The correlation 
      of VDR and MCP-1 expressions with the disease activity index of SLE (SLEDAI) of 
      the patients were analyzed. RESULTS: The expressions of VDR mRNA and protein in 
      the PBMCs were significantly lower in patients with SLE than in the healthy 
      individuals (P < 0.01), and that in the patients with active disease was lower 
      than in remission (P < 0.05). The MCP-1 mRNA expression in the PBMCs and its 
      serum levels were significantly increased in SLE patients as compared with the 
      healthy individuals (P < 0.01), and the increase was significantly more obvious 
      in the patients with active disease than in those in remission (P < 0.01). 
      Pearson correlation analysis showed that VDR mRNA in the PBMCs was negatively 
      correlated with SLEDAI (r=-0.417, P=0.001); negative correlations were also found 
      between VDR mRNA and MCP-1 mRNA(r=-0.554, P=0.000) and between VDR protein 
      expression and serum MCP-1 level (r=-0.400, P=0.028). CONCLUSIONS: The 
      down-regulation of VDR expression in the PBMC is negatively correlated with the 
      disease activity of SLE. VDR may play an important role in the pathogenesis of 
      SLE by affecting the expression of MCP-1.
FAU - Huang, Yuxi
AU  - Huang Y
AD  - Department of Nephrology and Rheumatology, Third Xiangya Hospital, Central South 
      University, Changsha 410013, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Nephrology and Rheumatology, Third Xiangya Hospital, Central South 
      University, Changsha 410013, China.
FAU - Zhang, Shuang
AU  - Zhang S
AD  - Department of Nephrology and Rheumatology, Third Xiangya Hospital, Central South 
      University, Changsha 410013, China.
FAU - Sun, Jian
AU  - Sun J
AD  - Department of Nephrology and Rheumatology, Third Xiangya Hospital, Central South 
      University, Changsha 410013, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Calcitriol)
RN  - 0 (VDR protein, human)
SB  - IM
MH  - Case-Control Studies
MH  - Chemokine CCL2/*metabolism
MH  - Humans
MH  - Leukocytes, Mononuclear/*metabolism
MH  - Lupus Erythematosus, Systemic/*metabolism
MH  - RNA, Messenger
MH  - Receptors, Calcitriol/*metabolism
PMC - PMC7040761
OTO - NOTNLM
OT  - disease activity
OT  - monocyte chemoattractant protein-1
OT  - systemic lupus erythemaotsus
OT  - vitamin D receptor
EDAT- 2020/05/08 06:00
MHDA- 2020/07/01 06:00
PMCR- 2020/01/20
CRDT- 2020/05/08 06:00
PHST- 2020/05/08 06:00 [entrez]
PHST- 2020/05/08 06:00 [pubmed]
PHST- 2020/07/01 06:00 [medline]
PHST- 2020/01/20 00:00 [pmc-release]
AID - nfykdxxb-40-1-99 [pii]
AID - 10.12122/j.issn.1673-4254.2020.01.16 [doi]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2020 Jan 30;40(1):99-103. doi: 
      10.12122/j.issn.1673-4254.2020.01.16.