PMID- 32377518
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20220414
IS  - 2314-6753 (Electronic)
IS  - 2314-6745 (Print)
VI  - 2020
DP  - 2020
TI  - Banxia Xiexin Decoction Ameliorates t-BHP-Induced Apoptosis in Pancreatic Beta 
      Cells by Activating the PI3K/AKT/FOXO1 Signaling Pathway.
PG  - 3695689
LID - 10.1155/2020/3695689 [doi]
LID - 3695689
AB  - BACKGROUND: Banxia Xiexin Decoction (BXXD) reportedly regulates glycolipid 
      metabolism and inhibits pancreatic beta-cell apoptosis. This study is aimed at 
      investigating the protective effect of BXXD on tert-butyl hydroperoxide- (t-BHP-) 
      induced apoptosis in MIN6 cells and the underlying mechanisms. METHODS: MIN6 
      cells were preincubated with BXXD or liraglutide (Li) with or without PI3K 
      inhibitor LY294002 (LY) for 12 h, following which t-BHP was added to induce MIN6 
      cell apoptosis. The protective effects of BXXD on MIN6 cells were evaluated by 
      detecting cell viability and proliferation and glucose-stimulated insulin 
      secretion (GSIS). The antiapoptotic effects were evaluated by Hoechst 33342 
      staining and terminal deoxynucleotidyl transferase dUTP nick end labeling assay 
      (TUNEL). Malondialdehyde and glutathione peroxidase content and superoxide 
      dismutase activity were measured using commercial kits. The expression of 
      PI3K/AKT/FOXO1 signaling pathway-related signal molecules, and that of apoptotic 
      indicators Bax, P27, and Caspase-3, was quantified using western blotting. 
      RESULTS: Preincubation with BXXD significantly improved t-BHP-induced 
      proliferation inhibition and apoptosis and enhanced GSIS. t-BHP induced the 
      generation of reactive oxygen species and inhibited the activities of antioxidant 
      enzymes, which could be neutralized by pretreatment with BXXD. BXXD promoted the 
      phosphorylation of AKT and FOXO1 in t-BHP-induced MIN6 cells. Moreover, BXXD 
      attenuated the expression of related apoptotic indicators Bax, P27, and 
      Caspase-3. LY abolished these effects of BXXD. CONCLUSION: BXXD protected MIN6 
      cells against t-BHP-induced apoptosis and improved insulin secretory function 
      through modulation of the PI3K/AKT pathway and the downstream FOXO1, thus 
      suggesting a novel therapeutic approach for type 2 diabetes mellitus (T2DM).
CI  - Copyright (c) 2020 Li-juan Du et al.
FAU - Du, Li-Juan
AU  - Du LJ
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
FAU - Pang, Bing
AU  - Pang B
AUID- ORCID: 0000-0002-0069-5055
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
FAU - Tan, Yu-Meng
AU  - Tan YM
AUID- ORCID: 0000-0002-1714-636X
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
FAU - Yang, Ya-Nan
AU  - Yang YN
AUID- ORCID: 0000-0003-3218-1566
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
FAU - Zhang, Mei-Zhen
AU  - Zhang MZ
AUID- ORCID: 0000-0002-7740-8376
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
FAU - Pang, Qing
AU  - Pang Q
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
FAU - Sun, Min
AU  - Sun M
AUID- ORCID: 0000-0002-8528-0050
AD  - School of Life Sciences, Anhui University, Hefei, China.
FAU - Ni, Qing
AU  - Ni Q
AUID- ORCID: 0000-0002-4786-4901
AD  - Department of Endocrinology, Guang'anmen Hospital of China Academy of Chinese 
      Medical Sciences, Beijing, China.
LA  - eng
PT  - Journal Article
DEP - 20200417
PL  - England
TA  - J Diabetes Res
JT  - Journal of diabetes research
JID - 101605237
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Forkhead Box Protein O1)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (banxia xiexin decoction)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 955VYL842B (tert-Butylhydroperoxide)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Caspase 3/metabolism
MH  - Cell Line
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Forkhead Box Protein O1/metabolism
MH  - Glutathione Peroxidase/metabolism
MH  - Insulin-Secreting Cells/*drug effects/metabolism
MH  - Malondialdehyde/metabolism
MH  - Mice
MH  - Oxidative Stress/drug effects
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphorylation/drug effects
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/*drug effects
MH  - Superoxide Dismutase/metabolism
MH  - tert-Butylhydroperoxide/*pharmacology
PMC - PMC7191444
COIS- No competing financial interests existed.
EDAT- 2020/05/08 06:00
MHDA- 2021/02/17 06:00
PMCR- 2020/04/17
CRDT- 2020/05/08 06:00
PHST- 2019/12/27 00:00 [received]
PHST- 2020/03/20 00:00 [revised]
PHST- 2020/03/31 00:00 [accepted]
PHST- 2020/05/08 06:00 [entrez]
PHST- 2020/05/08 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/04/17 00:00 [pmc-release]
AID - 10.1155/2020/3695689 [doi]
PST - epublish
SO  - J Diabetes Res. 2020 Apr 17;2020:3695689. doi: 10.1155/2020/3695689. eCollection 
      2020.