PMID- 32379910
OWN - NLM
STAT- MEDLINE
DCOM- 20210128
LR  - 20210128
IS  - 1439-0531 (Electronic)
IS  - 0936-6768 (Linking)
VI  - 55
IP  - 7
DP  - 2020 Jul
TI  - Sexing of pre-implantation ovine embryos through polymerase chain reaction-based 
      amplification of GAPDH, SRY and AMEL genes.
PG  - 885-892
LID - 10.1111/rda.13699 [doi]
AB  - The ability to identify the sex of embryo and control of sex ratio has a great 
      commercial importance to livestock industry. Prediction of embryonic sex could be 
      useful in the management decisions of sex selection in breeding programs. Several 
      methods have been attempted to determine the sex but the polymerase chain 
      reaction (PCR)-based sexing method is generally favoured, as it is cost 
      effective, simple and reliable. The aim of the present study was to identify sex 
      of sheep embryos produced in vitro through amplification of glyceraldehyde 
      3-phosphate dehydrogenase (GAPDH), sex-determining region Y (SRY) and amelogenin 
      genes present in genomic DNA (gDNA) of embryos through PCR. To avoid false 
      interpretation of the result by no amplification of SRY in female embryos, a 
      duplex PCR was approached to amplify combinedly SRY and GAPDH genes. Sex-specific 
      blood was used in PCR as positive control. In vitro sheep embryos were produced 
      as per standardized protocol of laboratory. Sexing of sex-specific blood and in 
      vitro produced embryos were approached though PCR to amplify the respective genes 
      using gDNA present in the sample without its traditional isolation. The accuracy 
      of sex prediction for embryos was 100% by this procedure.
CI  - (c) 2020 Blackwell Verlag GmbH.
FAU - Mishra, Ashish
AU  - Mishra A
AUID- ORCID: 0000-0001-6597-4162
AD  - ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India.
FAU - Dhali, Arindam
AU  - Dhali A
AUID- ORCID: 0000-0002-9734-8796
AD  - ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India.
FAU - Reddy, Ippala J
AU  - Reddy IJ
AD  - ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India.
FAU - Kolte, Atul P
AU  - Kolte AP
AD  - ICAR-National Institute of Animal Nutrition and Physiology, Bangalore, India.
LA  - eng
GR  - APR 3.15/ICAR-NIANP/
PT  - Journal Article
DEP - 20200526
PL  - Germany
TA  - Reprod Domest Anim
JT  - Reproduction in domestic animals = Zuchthygiene
JID - 9015668
RN  - 0 (Amelogenin)
RN  - 9007-49-2 (DNA)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Amelogenin/genetics/metabolism
MH  - Animals
MH  - DNA/blood/genetics
MH  - Embryo, Mammalian
MH  - Female
MH  - Genes, sry/genetics
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/genetics/metabolism
MH  - Male
MH  - Polymerase Chain Reaction/methods/*veterinary
MH  - Sex Determination Analysis/methods/*veterinary
MH  - Sheep/*embryology
OTO - NOTNLM
OT  - AMEL
OT  - GAPDH
OT  - SRY
OT  - PCR
OT  - embryos
OT  - sex identification
EDAT- 2020/05/08 06:00
MHDA- 2021/01/29 06:00
CRDT- 2020/05/08 06:00
PHST- 2019/08/02 00:00 [received]
PHST- 2020/04/24 00:00 [accepted]
PHST- 2020/05/08 06:00 [pubmed]
PHST- 2021/01/29 06:00 [medline]
PHST- 2020/05/08 06:00 [entrez]
AID - 10.1111/rda.13699 [doi]
PST - ppublish
SO  - Reprod Domest Anim. 2020 Jul;55(7):885-892. doi: 10.1111/rda.13699. Epub 2020 May 
      26.