PMID- 32380247 OWN - NLM STAT- MEDLINE DCOM- 20210225 LR - 20240323 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 259 DP - 2020 Sep 15 TI - Effects of a rhizome aqueous extract of Dioscorea batatas and its bioactive compound, allantoin in high fat diet and streptozotocin-induced diabetic mice and the regulation of liver, pancreas and skeletal muscle dysfunction. PG - 112926 LID - S0378-8741(20)30919-3 [pii] LID - 10.1016/j.jep.2020.112926 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Dysfunction of glucose metabolism is associated with the occurrence of metabolic syndromes, including type 2 diabetes mellitus (T2DM). In this study, we investigated the anti-diabetic effects of yam aqueous extract and allantoin in high-fat-diet (HFD) and streptozotocin (STZ)-induced diabetic mice and the mechanism of action on the dysfunction of the liver, pancreas, and skeletal muscle. MATERIALS AND METHODS: Male C57BL/6 mice were induced into a diabetic condition by HFD for 16 weeks and a single injection of STZ (120 mg/kg) and then orally administered yam aqueous extract (500 and 1000 mg/kg) or allantoin (20 and 50 mg/kg) once daily for 4 weeks. The changes in physiological parameters, serological parameters, and morphology of tissues were investigated. The expression levels of antioxidant enzymes, biogenetic proteins, and myogenetic proteins were determined in the liver, pancreas and skeletal muscle tissues of mice. RESULTS: The administration of yam aqueous extract and allantoin at high doses in HFD/STZ-induced diabetic mice compared with the control group significantly decreased the increase in body weight, caloric intake, and water intake. Yam aqueous extract and allantoin significantly decreased high glucose and leptin, total cholesterol, triglyceride, low-density lipoprotein-cholesterol, aspartate transaminase, alanine aminotransferase levels and increased insulin and albumin levels in the plasma of mice. Yam aqueous extract and allantoin inhibited the structural damage of the liver with regard to fat accumulation, the pancreas with atrophy of Langerhans' islets, and skeletal muscle with regard to atrophy and significantly increased the expression of antioxidant enzymes and mitochondria-mediated biogenetic factors in the liver, pancreas, and muscle tissues. In addition, Yam aqueous extract and allantoin significantly increased the expression of myogenetic proteins in skeletal muscle tissues. CONCLUSION: Our results indicated that Yam aqueous extract and allantoin improve diabetic symptoms through the regulation of oxidation and glucose imbalance in liver, pancreas, and skeletal muscle tissues in mice. These findings suggest that Yam aqueous extract and allantoin can be used as antidiabetic factors in supplementary foods and medications for T2DM patients. CI - Copyright (c) 2020 The Authors. Published by Elsevier B.V. All rights reserved. FAU - Ma, Junnan AU - Ma J AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: nnzhgn@gmail.com. FAU - Meng, Xianglong AU - Meng X AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea; Experimental Teaching Center, College of Chinese Materia Medica, Shanxi University of Chinese Medicine, Jinzhong, 030619, China. Electronic address: sszywzh@126.com. FAU - Liu, Yi AU - Liu Y AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: liuyi980216@163.com. FAU - Yin, Cheng AU - Yin C AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: yincheng0926@gmail.com. FAU - Zhang, Tong AU - Zhang T AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: ztong1995.love@gmail.com. FAU - Wang, Piao AU - Wang P AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: 18234071171@163.com. FAU - Park, Yong-Ki AU - Park YK AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea; Korean Medicine R&D Center, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: yongki@dongguk.ac.kr. FAU - Jung, Hyo Won AU - Jung HW AD - Department of Herbology, College of Korean Medicine, Dongguk University, Gyeongju, 38066, South Korea; Korean Medicine R&D Center, Dongguk University, Gyeongju, 38066, South Korea. Electronic address: tenzing2@hanmail.net. LA - eng PT - Journal Article DEP - 20200504 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Plant Extracts) RN - 344S277G0Z (Allantoin) RN - 5W494URQ81 (Streptozocin) RN - EC 1.- (Oxidoreductases) SB - IM EIN - J Ethnopharmacol. 2024 Jun 12;327:118071. PMID: 38521693 MH - Allantoin/pharmacology MH - Animals MH - Diabetes Mellitus, Experimental/*drug therapy MH - *Dioscorea MH - Liver/*drug effects/enzymology/pathology MH - Male MH - Mice MH - Mice, Inbred C57BL MH - Muscle, Skeletal/*drug effects/enzymology MH - Oxidoreductases/metabolism MH - Pancreas/*drug effects/enzymology MH - Plant Extracts/*pharmacology MH - Rhizome MH - Streptozocin OTO - NOTNLM OT - Allantoin OT - Antioxidation OT - Dioscorea batatas OT - Mitochondrial biogenesis OT - Type 2 diabetes mellitus COIS- Declaration of competing interest The authors have no conflicts of interest to declare. EDAT- 2020/05/08 06:00 MHDA- 2021/02/26 06:00 CRDT- 2020/05/08 06:00 PHST- 2020/02/27 00:00 [received] PHST- 2020/04/16 00:00 [revised] PHST- 2020/04/26 00:00 [accepted] PHST- 2020/05/08 06:00 [pubmed] PHST- 2021/02/26 06:00 [medline] PHST- 2020/05/08 06:00 [entrez] AID - S0378-8741(20)30919-3 [pii] AID - 10.1016/j.jep.2020.112926 [doi] PST - ppublish SO - J Ethnopharmacol. 2020 Sep 15;259:112926. doi: 10.1016/j.jep.2020.112926. Epub 2020 May 4.