PMID- 32383265
OWN - NLM
STAT- MEDLINE
DCOM- 20210405
LR  - 20211204
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 235
IP  - 12
DP  - 2020 Dec
TI  - NLRC3 inhibits PDGF-induced PASMCs proliferation via PI3K-mTOR pathway.
PG  - 9557-9567
LID - 10.1002/jcp.29763 [doi]
AB  - Few studies about nucleotide-oligomerization domain-like receptor subfamily C3 
      (NLRC3) in PASMCs have been conducted. This research aimed to investigate the 
      role of NLRC3 on platelet-derived growth factor (PDGF)-induced proliferation of 
      pulmonary artery smooth muscle cells (PASMCs) and its underlying mechanism. We 
      found that the proliferation of PASMCs stimulated with PDGF decreased when 
      phosphoinositide 3-kinase (PI3K) or mammalian target of rapamycin (mTOR) 
      inhibitors pretreatment. Overexpression of NLRC3 inhibited the proliferation of 
      PASMCs and the phosphorylation of PI3K and mTOR while knocking down NLRC3 
      reversed this effect. Targeted to PI3K or mTOR can also reverse the effect of 
      NLRC3. Activation of PI3K increased the phosphorylation of mTOR while inhibition 
      of PI3K reduced it. Our data suggest that PDGF can induce abnormal proliferation 
      of PASMCs, and NLRC3 suppresses activation of the PI3K-mTOR signaling thus 
      inhibits PASMCs proliferation. These findings unveiled the effect of NLRC3 as an 
      inhibitor of the PI3K-mTOR pathway mediating protection against PASMCs 
      proliferation.
CI  - (c) 2020 Wiley Periodicals LLC.
FAU - Zha, Li-Huang
AU  - Zha LH
AUID- ORCID: 0000-0002-8024-4501
AD  - Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 
      Hunan, China.
FAU - Zhou, Jun
AU  - Zhou J
AD  - Department of Medical Science Research Center, Xiangya Hospital, Central South 
      University, Changsha, Hunan, China.
FAU - Tan, Yilong
AU  - Tan Y
AD  - Department of Ultrasonography, Zhuzhou Hospital, Central South University, 
      Zhuzhou, Hunan, China.
FAU - Guo, Shuhong
AU  - Guo S
AD  - Department of Cardiology, Zhuzhou Hospital, Central South University, Zhuzhou, 
      Hunan, China.
FAU - Zhang, Men-Qiu
AU  - Zhang MQ
AD  - Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 
      Hunan, China.
FAU - Li, Sheng
AU  - Li S
AD  - Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 
      Hunan, China.
FAU - Yan, Peng
AU  - Yan P
AD  - Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 
      Hunan, China.
FAU - Yu, Zai-Xin
AU  - Yu ZX
AUID- ORCID: 0000-0002-3555-1887
AD  - Department of Cardiology, Xiangya Hospital, Central South University, Changsha, 
      Hunan, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200507
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Intercellular Signaling Peptides and Proteins)
RN  - 0 (NLRC3 protein, human)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation/drug effects
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Intercellular Signaling Peptides and Proteins/*genetics
MH  - Myocytes, Smooth Muscle/metabolism
MH  - Phosphatidylinositol 3-Kinases/*genetics/pharmacology
MH  - Phosphoinositide-3 Kinase Inhibitors/*pharmacology
MH  - Phosphorylation/drug effects
MH  - Platelet-Derived Growth Factor/pharmacology
MH  - Pulmonary Artery/growth & development/metabolism/pathology
MH  - Rats
MH  - TOR Serine-Threonine Kinases/antagonists & inhibitors/*genetics
OTO - NOTNLM
OT  - NLRC3
OT  - PASMCs
OT  - PI3K
OT  - mTOR
OT  - proliferation
EDAT- 2020/05/10 06:00
MHDA- 2021/04/07 06:00
CRDT- 2020/05/09 06:00
PHST- 2019/07/09 00:00 [received]
PHST- 2020/04/23 00:00 [accepted]
PHST- 2020/05/10 06:00 [pubmed]
PHST- 2021/04/07 06:00 [medline]
PHST- 2020/05/09 06:00 [entrez]
AID - 10.1002/jcp.29763 [doi]
PST - ppublish
SO  - J Cell Physiol. 2020 Dec;235(12):9557-9567. doi: 10.1002/jcp.29763. Epub 2020 May 
      7.