PMID- 32384777
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 8
IP  - 5
DP  - 2020 May 6
TI  - Endodontic-Like Oral Biofilms as Models for Multispecies Interactions in 
      Endodontic Diseases.
LID - 10.3390/microorganisms8050674 [doi]
LID - 674
AB  - Oral bacteria possess the ability to form biofilms on solid surfaces. After the 
      penetration of oral bacteria into the pulp, the contact between biofilms and pulp 
      tissue may result in pulpitis, pulp necrosis and/or periapical lesion. Depending 
      on the environmental conditions and the availability of nutrients in the pulp 
      chamber and root canals, mainly Gram-negative anaerobic microorganisms 
      predominate and form the intracanal endodontic biofilm. The objective of the 
      present study was to investigate the role of different substrates on biofilm 
      formation as well as the separate and collective incorporation of six endodontic 
      pathogens, namely Enterococcus faecalis, Staphylococcus aureus, Prevotella 
      nigrescens, Selenomonas sputigena, Parvimonas micra and Treponema denticola into 
      a nine-species "basic biofilm". This biofilm was formed in vitro as a standard 
      subgingival biofilm, comprising Actinomyces oris, Veillonella dispar, 
      Fusobacterium nucleatum, Streptococcus anginosus, Streptococcus oralis, 
      Prevotella intermedia, Campylobacter rectus, Porphyromonas gingivalis, and 
      Tannerella forsythia. The resulting endodontic-like biofilms were grown 64 h 
      under the same conditions on hydroxyapatite and dentin discs. After harvesting 
      the endodontic-like biofilms, the bacterial growth was determined using 
      quantitative real-time PCR, were labeled using fluorescence in situ hybridization 
      (FISH) and analyzed by confocal laser scanning microscopy (CLSM). The addition of 
      six endodontic pathogens to the "basic biofilm" induced a decrease in the cell 
      number of the "basic" species. Interestingly, C. rectus counts increased in 
      biofilms containing E. faecalis, S. aureus, P. nigrescens and S. sputigena, 
      respectively, both on hydroxyapatite and on dentin discs, whereas P. intermedia 
      counts increased only on dentin discs by addition of E. faecalis. The growth of 
      E. faecalis on hydroxyapatite discs and of E. faecalis and S. aureus on dentin 
      discs were significantly higher in the biofilm containing all species than in the 
      "basic biofilm". Contrarily, the counts of P. nigrescens, S. sputigena and P. 
      micra on hydroxyapatite discs as well as counts of P. micra and T. denticola on 
      dentin discs decreased in the all-species biofilm. Overall, all bacterial species 
      associated with endodontic infections were successfully incorporated into the 
      standard multispecies biofilm model both on hydroxyapatite and dentin discs. 
      Thus, future investigations on endodontic infections can rely on this newly 
      established endodontic-like multispecies biofilm model.
FAU - Lukic, Dejana
AU  - Lukic D
AD  - Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, 
      University of Zurich, 8032 Zurich, Switzerland.
FAU - Karygianni, Lamprini
AU  - Karygianni L
AD  - Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, 
      University of Zurich, 8032 Zurich, Switzerland.
FAU - Flury, Manuela
AU  - Flury M
AD  - Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, 
      University of Zurich, 8032 Zurich, Switzerland.
FAU - Attin, Thomas
AU  - Attin T
AD  - Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, 
      University of Zurich, 8032 Zurich, Switzerland.
FAU - Thurnheer, Thomas
AU  - Thurnheer T
AD  - Clinic of Conservative and Preventive Dentistry, Center of Dental Medicine, 
      University of Zurich, 8032 Zurich, Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20200506
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC7285038
OTO - NOTNLM
OT  - FISH
OT  - endodontic pathogens
OT  - endodontic-like multispecies biofilm
OT  - pulp necrosis
OT  - pulpitis
OT  - quantitative real-time PCR (qPCR), CLSM
COIS- The authors declare no competing interests.
EDAT- 2020/05/10 06:00
MHDA- 2020/05/10 06:01
PMCR- 2020/05/06
CRDT- 2020/05/10 06:00
PHST- 2020/04/03 00:00 [received]
PHST- 2020/04/29 00:00 [revised]
PHST- 2020/05/04 00:00 [accepted]
PHST- 2020/05/10 06:00 [entrez]
PHST- 2020/05/10 06:00 [pubmed]
PHST- 2020/05/10 06:01 [medline]
PHST- 2020/05/06 00:00 [pmc-release]
AID - microorganisms8050674 [pii]
AID - microorganisms-08-00674 [pii]
AID - 10.3390/microorganisms8050674 [doi]
PST - epublish
SO  - Microorganisms. 2020 May 6;8(5):674. doi: 10.3390/microorganisms8050674.