PMID- 32385772
OWN - NLM
STAT- MEDLINE
DCOM- 20210216
LR  - 20220311
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 47
IP  - 6
DP  - 2020 Jun
TI  - An investigation of the relationship between TMPRSS6 gene expression, genetic 
      variants and clinical findings in breast cancer.
PG  - 4225-4231
LID - 10.1007/s11033-020-05498-0 [doi]
AB  - Breast cancer is one of the most common types of cancer among women worldwide. 
      The TMPRSS6 (Transmembrane Serine Protease 6) gene encodes matriptase-2, which 
      plays an important role in iron hemostasis as the hepcidin regulator and may play 
      a role in breast cancer susceptibility. In this study, we examined the expression 
      levels of the TMPRSS6 gene in healthy tissues and tumor tissues of breast cancer 
      patients; and the relationship between these levels and pathological findings. 
      The relationship between TMPRSS6 polymorphisms (rs733655, rs5756506, rs2413450, 
      rs855791, rs2235324, rs4820268) and patients' hematological parameters. The gene 
      expression study encompassed 47 breast cancer patients and the gene polymorphism 
      study consisted of 181 breast cancer patients and 100 healthy controls. Gene 
      expression analysis was performed by qRT-PCR. The genotyping of TMPRSS6 
      polymorphisms was performed by RT-PCR. TMPRSS6 gene expression levels in tumor 
      tissues were found to be 1.88 times higher than the expression levels in the 
      control tissues. We examined the relationship between TMPRSS6 gene expression 
      levels and pathological data, statistically significant relationship was found 
      between patient's estrogen receptor (ER) and HER2 findings and TMPRSS6 gene 
      expression (respectively p = 0.02, p = 0.002). When the relationship between 
      TMPRSS6 gene polymorphisms related genotypes distributions and hematological 
      findings was investigated, a significant relationship was identified between mean 
      corpuscular hemoglobin concentration (MCHC) parameter and the polymorphism of 
      only the rs733655. According to our findings, the increase in TMPRSS6 gene 
      expression in cancerous tissues shows that matriptase-2 may be effective in the 
      cancer process. Thus TMPRSS6 gene polymorphisms may affect the disease process by 
      affecting the blood parameters of patients.
FAU - Mete, Meltem
AU  - Mete M
AD  - Department of Medical Biology, Medical Faculty of Cerrahpasa, Istanbul 
      University-Cerrahpasa, Istanbul, Turkey.
FAU - Trabulus, Didem Can
AU  - Trabulus DC
AD  - Department of General Surgery, Istanbul Training and Research Hospital, Istanbul, 
      Turkey.
FAU - Talu, Canan Kelten
AU  - Talu CK
AD  - Department of Pathology, Istanbul Training and Research Hospital, Istanbul, 
      Turkey.
FAU - Ozoran, Emre
AU  - Ozoran E
AD  - Department of General Surgery, Koc University Hospital, Istanbul, Turkey.
FAU - Mutlu, Tuba
AU  - Mutlu T
AD  - Department of Medical Biology, Medical Faculty of Cerrahpasa, Istanbul 
      University-Cerrahpasa, Istanbul, Turkey.
FAU - Tekin, Bulent
AU  - Tekin B
AD  - Department of Medical Biology, Medical Faculty of Cerrahpasa, Istanbul 
      University-Cerrahpasa, Istanbul, Turkey.
FAU - Guven, Mehmet
AU  - Guven M
AUID- ORCID: 0000-0002-8749-1708
AD  - Department of Medical Biology, Medical Faculty of Cerrahpasa, Istanbul 
      University-Cerrahpasa, Istanbul, Turkey. mgguven@istanbul.edu.tr.
LA  - eng
PT  - Journal Article
DEP - 20200508
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (Membrane Proteins)
RN  - E1UOL152H7 (Iron)
RN  - EC 3.4.21.- (Serine Endopeptidases)
RN  - EC 3.4.21.- (TMPRSS6 protein, human)
RN  - EC 3.4.21.- (matriptase 2)
SB  - IM
MH  - Adult
MH  - Breast Neoplasms/*genetics/metabolism
MH  - Female
MH  - Gene Expression
MH  - Genetic Predisposition to Disease
MH  - Genetic Variation
MH  - Genotype
MH  - Homeostasis/genetics
MH  - Humans
MH  - Iron/metabolism
MH  - Membrane Proteins/*genetics/metabolism
MH  - Middle Aged
MH  - Polymorphism, Single Nucleotide
MH  - Serine Endopeptidases/*genetics/metabolism
OTO - NOTNLM
OT  - Breast cancer
OT  - Gene expression
OT  - Matriptase-2
OT  - Polymorphisms
OT  - TMPRSS6
EDAT- 2020/05/10 06:00
MHDA- 2021/02/17 06:00
CRDT- 2020/05/10 06:00
PHST- 2020/03/02 00:00 [received]
PHST- 2020/05/03 00:00 [accepted]
PHST- 2020/05/10 06:00 [pubmed]
PHST- 2021/02/17 06:00 [medline]
PHST- 2020/05/10 06:00 [entrez]
AID - 10.1007/s11033-020-05498-0 [pii]
AID - 10.1007/s11033-020-05498-0 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2020 Jun;47(6):4225-4231. doi: 10.1007/s11033-020-05498-0. Epub 
      2020 May 8.