PMID- 32387557
OWN - NLM
STAT- MEDLINE
DCOM- 20210915
LR  - 20210915
IS  - 1879-0089 (Electronic)
IS  - 0145-305X (Linking)
VI  - 110
DP  - 2020 Sep
TI  - Splicing variants of grass carp (Ctenopharyngodon idellus) IL-21: Functions in 
      IgM(+) B cell proliferation and IgM secretion.
PG  - 103728
LID - S0145-305X(20)30007-0 [pii]
LID - 10.1016/j.dci.2020.103728 [doi]
AB  - In mammals, interleukin 21 (IL-21) is a type I four-helical bundle cytokine 
      produced by activated T cells that has pleiotropic functions on immune cells. 
      Although IL-21 has been discovered in fish, the splicing variants of this 
      cytokine and their functions on B cells are unclear. In this study, based on the 
      original transcript of grass carp IL-21 (named gcIL-21sv1 in this study), two 
      alternative splicing variants, named gcIL-21sv2 and gcIL-21sv3, were cloned and 
      characterized. The protein sequences of gcIL-21sv1 and gcIL-21sv2 consist of four 
      alpha-helixes, and only the six amino acid residues at the C-terminal are different. 
      Unlike gcIL-21sv1 and gcIL-21sv2, gcIL-21sv3 lacks the C-terminal region. The 
      expression analysis showed that gcIL-21sv1, gcIL-21sv2, and gcIL-21sv3 were 
      constitutively expressed in all the tested tissues, and their expression could be 
      significantly up-regulated by LPS and Poly (I:C) in head kidney leukocytes 
      (HKLs), with the fold change of gcIL-21sv1 being higher than that of gcIL-21sv2 
      and gcIL-21sv3. Recombinant gcIL-21sv1 and gcIL-21sv2, but not gcIL-21sv3, could 
      induce the proliferation of IgM(+) B cells and the secretion of IgM, with the 
      activity of gcIL-21sv1 being stronger than that of gcIL-21sv2, indicating that 
      the C-terminal region plays important roles in the function of gcIL-21. Taken 
      together, this study found that, like IL-21 in human and mouse, IL-21 splicing 
      variants also exist in fish, and the regulatory activities of these variants in 
      humoral immunity are differ, suggesting that grass carp may balance the immune 
      response mediated by IL-21 through alternative splicing.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Cui, Zheng-Wei
AU  - Cui ZW
AD  - Key Laboratory of Fishery Drug Development, Ministry of Agriculture, Key 
      Laboratory of Aquatic Animal Immune Technology, Pearl River Fisheries Research 
      Institute, Chinese Academy of Fishery Sciences, Guangzhou, 510380, China.
FAU - Zhang, Xiang-Yang
AU  - Zhang XY
AD  - Key Laboratory of Marine Biotechnology of Fujian Province, Institute of 
      Oceanology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
FAU - Chen, Xiao-Hua
AU  - Chen XH
AD  - Department of Clinical Laboratory, General Hospital of Central Theater Command, 
      PLA, Wuhan, 430070, China. Electronic address: cxhniuniu@163.com.
FAU - Zhang, Xu-Jie
AU  - Zhang XJ
AD  - State Key Laboratory of Agricultural Microbiology, College of Fisheries, Huazhong 
      Agricultural University, Wuhan, 430070, China. Electronic address: 
      xujiezhang@mail.hzau.edu.cn.
FAU - Zhang, Yong-An
AU  - Zhang YA
AD  - State Key Laboratory of Agricultural Microbiology, College of Fisheries, Huazhong 
      Agricultural University, Wuhan, 430070, China; Laboratory for Marine Biology and 
      Biotechnology, Qingdao National Laboratory for Marine Science and Technology, 
      Qingdao, 266071, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200506
PL  - United States
TA  - Dev Comp Immunol
JT  - Developmental and comparative immunology
JID - 7708205
RN  - 0 (Fish Proteins)
RN  - 0 (Immunoglobulin M)
RN  - 0 (Interleukins)
RN  - 0 (Lipopolysaccharides)
RN  - MKM3CA6LT1 (interleukin-21)
RN  - O84C90HH2L (Poly I-C)
SB  - IM
MH  - Alternative Splicing
MH  - Animals
MH  - B-Lymphocytes/*immunology
MH  - Carps/*immunology
MH  - Cell Proliferation
MH  - Cloning, Molecular
MH  - Fish Proteins/genetics/*metabolism
MH  - Head Kidney/*immunology
MH  - Humans
MH  - Immunity, Humoral
MH  - Immunoglobulin M/*metabolism
MH  - Interleukins/genetics/*metabolism
MH  - Leukocytes/*immunology
MH  - Lipopolysaccharides/immunology
MH  - Lymphocyte Activation
MH  - Mice
MH  - Poly I-C/immunology
OTO - NOTNLM
OT  - B cell
OT  - Grass carp
OT  - IL-21
OT  - IgM
OT  - Splicing variant
EDAT- 2020/05/11 06:00
MHDA- 2021/09/16 06:00
CRDT- 2020/05/11 06:00
PHST- 2020/01/07 00:00 [received]
PHST- 2020/03/03 00:00 [revised]
PHST- 2020/04/29 00:00 [accepted]
PHST- 2020/05/11 06:00 [pubmed]
PHST- 2021/09/16 06:00 [medline]
PHST- 2020/05/11 06:00 [entrez]
AID - S0145-305X(20)30007-0 [pii]
AID - 10.1016/j.dci.2020.103728 [doi]
PST - ppublish
SO  - Dev Comp Immunol. 2020 Sep;110:103728. doi: 10.1016/j.dci.2020.103728. Epub 2020 
      May 6.