PMID- 32387611
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
DP  - 2020 May 6
TI  - Structural characterization and biological activities of a novel polysaccharide 
      containing N-acetylglucosamine from Ganoderma sinense.
LID - S0141-8130(20)33174-3 [pii]
LID - 10.1016/j.ijbiomac.2020.05.028 [doi]
AB  - A novel homogeneous heteropolysaccharide (GSPB70-S) with a molecular weight of 
      2.87 kDa was isolated from Ganoderma sinense. Structural analysis showed that 
      GSPB70-S was composed of glucose, glucosamine, mannose, and galactose with a 
      molar ratio of 12.90:3.70:2.26:1.00. The repeating structure units of GSPB70-S 
      were characterized by the combined application of chemical methods and nuclear 
      magnetic resonance. GSPB70-S contains a backbone of 
      -->3)-beta-D-Glcp-(1 --> 4)-alpha-D-GlcpNAc-(1 --> 4)-alpha-D-Manp-(1 --> 3)-beta-D-Glcp-(1-->, with 
      branches of beta-D-Glcp-(1-->, alpha-D-GlcpNAc-(1 --> and -->4)-alpha-D-Galp-(1-->. Scanning 
      electron microscope (SEM) showed that GSPB70-S presented a long strip shape with 
      different thicknesses, and there were many lamellar substances on the surface. 
      Biological research showed that GSPB70-S inhibited the activity of alpha-glucosidase 
      in vitro, increased the viability of RAW 264.7 macrophages, and promoted the 
      release of NO. In addition, GSPB70-S showed good abilities to scavenge free 
      radicals.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Chen, Yiyu
AU  - Chen Y
AD  - Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 
      Huangpu Avenue West, Guangzhou 510632, China.
FAU - Ou, Xiaozheng
AU  - Ou X
AD  - Department of Natural Product Chemistry, Jinan University, 601 Huangpu Avenue 
      West, Guangzhou 510632, China.
FAU - Yang, Jianing
AU  - Yang J
AD  - Department of Pharmacology, Jinan University, 601 Huangpu Avenue West, Guangzhou 
      510632, China.
FAU - Bi, Sixue
AU  - Bi S
AD  - Department of Pharmacology, Jinan University, 601 Huangpu Avenue West, Guangzhou 
      510632, China.
FAU - Peng, Bao
AU  - Peng B
AD  - Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 
      Huangpu Avenue West, Guangzhou 510632, China.
FAU - Wen, Yao
AU  - Wen Y
AD  - Department of Natural Product Chemistry, Jinan University, 601 Huangpu Avenue 
      West, Guangzhou 510632, China.
FAU - Song, Liyan
AU  - Song L
AD  - Department of Pharmacology, Jinan University, 601 Huangpu Avenue West, Guangzhou 
      510632, China. Electronic address: tsly@jnu.edu.cn.
FAU - Li, Chunlei
AU  - Li C
AD  - Department of Natural Product Chemistry, Jinan University, 601 Huangpu Avenue 
      West, Guangzhou 510632, China.
FAU - Yu, Rongmin
AU  - Yu R
AD  - Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 
      Huangpu Avenue West, Guangzhou 510632, China; Department of Natural Product 
      Chemistry, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China. 
      Electronic address: tyrm@jnu.edu.cn.
FAU - Zhu, Jianhua
AU  - Zhu J
AD  - Biotechnological Institute of Chinese Materia Medica, Jinan University, 601 
      Huangpu Avenue West, Guangzhou 510632, China; Department of Natural Product 
      Chemistry, Jinan University, 601 Huangpu Avenue West, Guangzhou 510632, China. 
      Electronic address: tzhujh@jnu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200506
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
SB  - IM
OTO - NOTNLM
OT  - Biological activities
OT  - Ganoderma sinense
OT  - Heteropolysaccharide
OT  - Structure characterization
COIS- Declaration of competing interest No conflict of interest exists in this work.
EDAT- 2020/05/11 06:00
MHDA- 2020/05/11 06:00
CRDT- 2020/05/11 06:00
PHST- 2020/01/10 00:00 [received]
PHST- 2020/04/21 00:00 [revised]
PHST- 2020/05/04 00:00 [accepted]
PHST- 2020/05/11 06:00 [entrez]
PHST- 2020/05/11 06:00 [pubmed]
PHST- 2020/05/11 06:00 [medline]
AID - S0141-8130(20)33174-3 [pii]
AID - 10.1016/j.ijbiomac.2020.05.028 [doi]
PST - aheadofprint
SO  - Int J Biol Macromol. 2020 May 6:S0141-8130(20)33174-3. doi: 
      10.1016/j.ijbiomac.2020.05.028.