PMID- 32387845
OWN - NLM
STAT- MEDLINE
DCOM- 20201217
LR  - 20201217
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 198
DP  - 2020 Jul 15
TI  - Heavy metals-resistant bacteria (HM-RB): Potential bioremediators of heavy 
      metals-stressed Spinacia oleracea plant.
PG  - 110685
LID - S0147-6513(20)30524-8 [pii]
LID - 10.1016/j.ecoenv.2020.110685 [doi]
AB  - Microorganism technologies can provide a potential alternative to traditional 
      methods of removing heavy metals to conserve agricultural soils. This study aimed 
      to identify and characterize heavy metals-resistant bacteria (HM-RB) isolated 
      from industry-affected soil and their desired impact as bioremediators of heavy 
      metals-stressed spinach plants. Three of 135 isolates were selected based on a 
      high level of resistance to heavy metals. Based on morphological and biochemical 
      characteristics, the selected isolates were identified as Bacillus subtilis 
      subsp. spizizenii DSM 15029 T DSM (MA3), Paenibacillus jamilae DSM 13815 T DSM 
      (LA22), or Pseudomonas aeruginosa DSM 1117 DSM (SN36). Experiments were 
      implemented to investigate the three isolated HM-RB ability on improving 
      attributes of growth, physio-biochemistry, and components of the antioxidant 
      defense system of spinach plant exposed to the stress of cadmium (Cd(2+); 2 mM), 
      lead (Pb(2+); 2 mM) or 2 mM Cd(2+)+2 mM Pb(2+). Compared to control, Cd(2+) or 
      Pb(2+) stress markedly lowered plant fresh and dry weights, leaf contents of 
      chlorophylls and carotenoids, rates of transpiration (Tr), net photosynthesis 
      (Pn) and stomatal conductance (gs), relative water content (RWC), and membrane 
      stability index (MSI). In contrast, contents of alpha.tochopherol (alpha.TOC), ascorbic 
      acid (AsA), glutathione (GSH), proline, soluble sugars, Cd(2+), and Pb(2+), as 
      well as activities of enzymatic and non-enzymatic antioxidants were markedly 
      elevated. The application of HM-RB promoted the tolerance to heavy metal stress 
      in spinach plants by improving Tr, Pn, gs, RWC, and MSI, while activities of 
      enzymatic and non-enzymatic antioxidants were suppressed. These results reflected 
      positively in promoting plant growth under heavy metal stress. Therefore, the 
      application of HM-RB as potential bioremediators may be a promising strategy for 
      promoting plant growth and productivity under heavy metal stress.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Desoky, El-Sayed M
AU  - Desoky EM
AD  - Botany Department, Faculty of Agriculture, Zagazig University, 44511, Zagazig, 
      Egypt.
FAU - Merwad, Abdel-Rahman M
AU  - Merwad AM
AD  - Soil Science Department, Faculty of Agriculture, Zagazig University, 44511, 
      Zagazig, Egypt.
FAU - Semida, Wael M
AU  - Semida WM
AD  - Horticulture Department, Faculty of Agriculture, Fayoum University, 63514, 
      Fayoum, Egypt.
FAU - Ibrahim, Seham A
AU  - Ibrahim SA
AD  - Botany Department, Faculty of Agriculture, Zagazig University, 44511, Zagazig, 
      Egypt.
FAU - El-Saadony, Mohamed T
AU  - El-Saadony MT
AD  - Microbiology Department, Faculty of Agriculture, Zagazig University, 44511, 
      Zagazig, Egypt.
FAU - Rady, Mostafa M
AU  - Rady MM
AD  - Botany Department, Faculty of Agriculture, Fayoum University, 63514, Fayoum, 
      Egypt. Electronic address: mmr02@fayoum.edu.eg.
LA  - eng
PT  - Journal Article
DEP - 20200506
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Antioxidants)
RN  - 0 (Metals, Heavy)
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 00BH33GNGH (Cadmium)
RN  - 1406-65-1 (Chlorophyll)
RN  - GAN16C9B8O (Glutathione)
RN  - PQ6CK8PD0R (Ascorbic Acid)
RN  - Bacillus subtilis subsp. spizizenii
RN  - Paenibacillus jamilae
SB  - IM
MH  - Agriculture
MH  - Antioxidants
MH  - Ascorbic Acid
MH  - Bacillus/physiology
MH  - *Biodegradation, Environmental
MH  - Cadmium
MH  - Chlorophyll
MH  - Glutathione
MH  - Metals, Heavy/*analysis
MH  - Paenibacillus/physiology
MH  - Photosynthesis
MH  - Plant Leaves/chemistry
MH  - Soil
MH  - Soil Pollutants/analysis/*toxicity
MH  - Spinacia oleracea/microbiology/*physiology
OTO - NOTNLM
OT  - Antioxidants
OT  - Bacteria
OT  - Bioremediation
OT  - Heavy metal stress
OT  - Photosynthetic efficiency
OT  - Spinach
COIS- Declaration of competing interest The authors have no conflict of interest.
EDAT- 2020/05/11 06:00
MHDA- 2020/12/18 06:00
CRDT- 2020/05/11 06:00
PHST- 2020/02/12 00:00 [received]
PHST- 2020/03/19 00:00 [revised]
PHST- 2020/04/26 00:00 [accepted]
PHST- 2020/05/11 06:00 [pubmed]
PHST- 2020/12/18 06:00 [medline]
PHST- 2020/05/11 06:00 [entrez]
AID - S0147-6513(20)30524-8 [pii]
AID - 10.1016/j.ecoenv.2020.110685 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2020 Jul 15;198:110685. doi: 
      10.1016/j.ecoenv.2020.110685. Epub 2020 May 6.