PMID- 32387976
OWN - NLM
STAT- MEDLINE
DCOM- 20210104
LR  - 20210104
IS  - 1090-2163 (Electronic)
IS  - 0008-8749 (Linking)
VI  - 352
DP  - 2020 Jun
TI  - Cytokine stimulation of the choriocarcinoma cell line JEG-3 leads to alterations 
      in the HLA-G expression profile.
PG  - 104110
LID - S0008-8749(19)30423-X [pii]
LID - 10.1016/j.cellimm.2020.104110 [doi]
AB  - The checkpoint molecule human leukocyte antigen (HLA)-G has restricted tissue 
      expression, and plays a role in the establishment of maternal tolerance to the 
      semi-allogenic fetus during pregnancy by expression on the trophoblast cells in 
      the placenta. HLA-G exists in at least seven well-described mRNA isoforms, of 
      which four are membrane-bound and three soluble. Regulation of the tissue 
      expression of HLA-G and its isoforms is relatively unknown. Therefore, it is 
      important to understand the regulation of HLA-G, and the HLA-G(+) choriocarcinoma 
      cell line JEG-3 is a widely used cellular model. We hypothesized that cytokines 
      present in the microenvironment can regulate the HLA-G expression profile. In the 
      present study, we systematically stimulated JEG-3 cells with various 
      concentrations of IL-2, IL-4 IL-6, IL-10, IL-12, IL-15, IL-17A, TGF-beta(1), TNF-alpha 
      and IFN-gamma1b. The results suggest that IFN-gamma plays a role in maintenance of HLA-G 
      expression, while IL-10 might be involved in regulation of the isoform profile.
CI  - Copyright (c) 2020 Elsevier Inc. All rights reserved.
FAU - Persson, Gry
AU  - Persson G
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
FAU - Bork, Julie Birgit Siig
AU  - Bork JBS
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
FAU - Isgaard, Cecilie
AU  - Isgaard C
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
FAU - Larsen, Tine Graakjaer
AU  - Larsen TG
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
FAU - Bordoy, Anna Maria
AU  - Bordoy AM
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
FAU - Bengtsson, Meghan Sand
AU  - Bengtsson MS
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
FAU - Hviid, Thomas Vauvert Faurschou
AU  - Hviid TVF
AD  - Center for Immune Regulation and Reproductive Immunology (CIRRI), The ReproHealth 
      Consortium ZUH, Department of Clinical Biochemistry, Zealand University Hospital, 
      and the Department of Clinical Medicine, University of Copenhagen, Denmark.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200407
PL  - Netherlands
TA  - Cell Immunol
JT  - Cellular immunology
JID - 1246405
RN  - 0 (Cytokines)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-G Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Line, Tumor
MH  - Choriocarcinoma/*immunology
MH  - Cytokines/metabolism
MH  - Gene Expression/drug effects
MH  - HLA Antigens/genetics/immunology/metabolism
MH  - HLA-G Antigens/*genetics/*immunology/metabolism
MH  - Histocompatibility Antigens Class I/genetics
MH  - Humans
MH  - Immune Tolerance
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Transcriptome/immunology
MH  - Trophoblasts/metabolism
MH  - Tumor Microenvironment
OTO - NOTNLM
OT  - Cancer
OT  - Cell line
OT  - Cytokine
OT  - HLA class Ib
OT  - HLA-G
OT  - Immune tolerance
OT  - Isoform
OT  - Preeclampsia
OT  - Pregnancy
EDAT- 2020/05/11 06:00
MHDA- 2021/01/05 06:00
CRDT- 2020/05/11 06:00
PHST- 2019/10/15 00:00 [received]
PHST- 2020/03/16 00:00 [revised]
PHST- 2020/04/05 00:00 [accepted]
PHST- 2020/05/11 06:00 [pubmed]
PHST- 2021/01/05 06:00 [medline]
PHST- 2020/05/11 06:00 [entrez]
AID - S0008-8749(19)30423-X [pii]
AID - 10.1016/j.cellimm.2020.104110 [doi]
PST - ppublish
SO  - Cell Immunol. 2020 Jun;352:104110. doi: 10.1016/j.cellimm.2020.104110. Epub 2020 
      Apr 7.