PMID- 32388392
OWN - NLM
STAT- MEDLINE
DCOM- 20210401
LR  - 20210401
IS  - 1873-4367 (Electronic)
IS  - 0927-7765 (Linking)
VI  - 193
DP  - 2020 Sep
TI  - Intracellular delivery of drugs by chitosan-based multi-liposomal complexes.
PG  - 111062
LID - S0927-7765(20)30292-7 [pii]
LID - 10.1016/j.colsurfb.2020.111062 [doi]
AB  - Positively charged linear chitosan molecules were cross-linked with 
      sulfate-anions to form chitosan nanoparticles which were used as a scaffold of 
      negatively charged cardiolipin/egg lecithin liposomes loaded with doxorubicin 
      (DOX). Thus formed multi-liposomal complexes (MLCs) containing 55 
      liposomes/chitosan and bearing a slight positive net charge effectively 
      transmitted DOX into the cytoplasm of cells in culture. The efficiency of DOX 
      delivery increased 4-5-fold upon drug incorporation in MLCs. Inside the cells, 
      the penetrated MLCs released DOX thus enabling its accumulation in the nuclei and 
      interaction with its intracellular target DNA leading to a decrease in cell 
      survival. The effects were demonstrated on 3T3 line of mouse fibroblasts, drug 
      sensitive tumor MCF-7 cells and drug resistant human ovarian carcinoma OVCAR-8 
      cells (formerly called MCF-7/ADR). Thus, MLC particles can be used for effective 
      delivery of payloads in cells of different origin.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Grozdova, Irina
AU  - Grozdova I
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, GSP-1, Leninskiye 
      Gory, 1, build. 3, 119991, Moscow, Russia.
FAU - Melik-Nubarov, Nikolay
AU  - Melik-Nubarov N
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, GSP-1, Leninskiye 
      Gory, 1, build. 3, 119991, Moscow, Russia.
FAU - Efimova, Anna
AU  - Efimova A
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, GSP-1, Leninskiye 
      Gory, 1, build. 3, 119991, Moscow, Russia. Electronic address: 
      ephimova@genebee.msu.su.
FAU - Ezhov, Alexander
AU  - Ezhov A
AD  - Faculty of Physics, M.V. Lomonosov Moscow State University, GSP-1, Leninskiye 
      Gory, 1, build. 2, 119991, Moscow, Russia.
FAU - Krivtsov, George
AU  - Krivtsov G
AD  - I.I. Mechnikov Research Institute of Vaccines and Sera, M. Kazenny per., 5a, 
      105064 Moscow, Russia.
FAU - Litmanovich, Ekaterina
AU  - Litmanovich E
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, GSP-1, Leninskiye 
      Gory, 1, build. 3, 119991, Moscow, Russia.
FAU - Yaroslavov, Alexander
AU  - Yaroslavov A
AD  - Faculty of Chemistry, M.V. Lomonosov Moscow State University, GSP-1, Leninskiye 
      Gory, 1, build. 3, 119991, Moscow, Russia.
LA  - eng
PT  - Journal Article
DEP - 20200505
PL  - Netherlands
TA  - Colloids Surf B Biointerfaces
JT  - Colloids and surfaces. B, Biointerfaces
JID - 9315133
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Drug Carriers)
RN  - 0 (Liposomes)
RN  - 80168379AG (Doxorubicin)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - 3T3 Cells
MH  - Animals
MH  - Antibiotics, Antineoplastic/chemistry/*pharmacology
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - Chitosan/*chemistry
MH  - Doxorubicin/chemistry/*pharmacology
MH  - Drug Carriers/chemistry
MH  - *Drug Delivery Systems
MH  - Drug Screening Assays, Antitumor
MH  - Female
MH  - Humans
MH  - Liposomes/chemistry
MH  - Mice
MH  - Microscopy, Confocal
MH  - Particle Size
MH  - Surface Properties
OTO - NOTNLM
OT  - Chitosan
OT  - Doxorubicin
OT  - Drug delivery
OT  - Liposomes
OT  - Multi-liposomal complexes
OT  - Multidrug resistance
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/05/11 06:00
MHDA- 2021/04/02 06:00
CRDT- 2020/05/11 06:00
PHST- 2020/01/25 00:00 [received]
PHST- 2020/03/30 00:00 [revised]
PHST- 2020/04/15 00:00 [accepted]
PHST- 2020/05/11 06:00 [pubmed]
PHST- 2021/04/02 06:00 [medline]
PHST- 2020/05/11 06:00 [entrez]
AID - S0927-7765(20)30292-7 [pii]
AID - 10.1016/j.colsurfb.2020.111062 [doi]
PST - ppublish
SO  - Colloids Surf B Biointerfaces. 2020 Sep;193:111062. doi: 
      10.1016/j.colsurfb.2020.111062. Epub 2020 May 5.