PMID- 32389166
OWN - NLM
STAT- MEDLINE
DCOM- 20200813
LR  - 20200813
IS  - 1007-8738 (Print)
IS  - 1007-8738 (Linking)
VI  - 36
IP  - 3
DP  - 2020 Mar
TI  - [CpG ODN synergistically promotes proliferation and migration of mouse RAW264.7 
      macrophages induced by lipopolysaccharide].
PG  - 198-204
AB  - Objective To investigate the effects of CpG oligodeoxynucleotide (CpG ODN) on the 
      proliferation and migration of macrophages induced by lipopolysaccharide (LPS) 
      and its mechanism. Methods In vitro inflammatory cell model was established by 1 
      mg/L LPS added into the culture medium of mouse RAW264.7 macrophages. CCK-8 assay 
      was performed to assess the effect of CpG ODN (500 nmol/L) on the proliferation 
      of macrophages induced by LPS; Transwell(TM) assay was used to measure the effect 
      of CpG ODN (500 nmol/L) on the migration of macrophages induced by LPS. Western 
      blot analysis was performed to detect the phosphorylation of p38 
      mitogen-activated protein kinase (p38MAPK), c-Jun N-terminal kinase (JNK), 
      extracellular regulated protein kinases (ERK) and nuclear factor kappa-B subunits 
      65 (NF-kappaBp65). The specific inhibitors for p38MAPK (SB203580), JNK (SP600125), 
      ERK (PD98059) and NF-kappaBp65 (BAY11-7082) were used to further explore the possible 
      mechanism underlying the effects of CpG ODN. Real-time PCR was used to detect the 
      role of CpG ODN in LPS-induced transcription of COX2 and MCP-1 in macrophages. 
      Results CpG ODN synergistically enhanced the proliferation and migration of 
      LPS-stimulated macrophages and promoted the transcription of COX2 and MCP-1. It 
      also selectively enhanced the phosphorylation of JNK and ERK proteins in MAPK 
      signaling pathway. To blockage JNK and ERK signaling pathways with its specific 
      inhibitors dramatically inhibited the effects of CpG ODN. Conclusion CpG ODN can 
      synergistically promote the proliferation and migration of LPS-stimulated 
      macrophages through JNK and ERK pathway as well as the transcription of COX2 and 
      MCP-1.
FAU - Yang, Chunxiu
AU  - Yang C
AD  - Guangdong Provincial Key Laboratory of Proteomics, Southern Medical University, 
      Guangzhou 510515, China.
FAU - Qu, Yibai
AU  - Qu Y
AD  - Guangdong Provincial Key Laboratory of Proteomics, Southern Medical University, 
      Guangzhou 510515, China.
FAU - Yan, Zhengzheng
AU  - Yan Z
AD  - Department of Anesthesiology, Nanfang Hospital, Southern Medical University, 
      Guangzhou 510515, China. *Corresponding author, E-mail: yzzaici888@163.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi
JT  - Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular 
      immunology
JID - 101139110
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Oligodeoxyribonucleotides)
RN  - 0 (Rela protein, mouse)
RN  - 0 (Transcription Factor RelA)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 2.7.11.24 (JNK Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
SB  - IM
MH  - Animals
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Chemokine CCL2
MH  - *CpG Islands
MH  - Cyclooxygenase 2
MH  - JNK Mitogen-Activated Protein Kinases
MH  - Lipopolysaccharides
MH  - MAP Kinase Signaling System
MH  - Macrophages/*cytology/drug effects
MH  - Mice
MH  - Oligodeoxyribonucleotides/*pharmacology
MH  - RAW 264.7 Cells
MH  - Transcription Factor RelA
MH  - p38 Mitogen-Activated Protein Kinases
EDAT- 2020/05/12 06:00
MHDA- 2020/08/14 06:00
CRDT- 2020/05/12 06:00
PHST- 2020/05/12 06:00 [entrez]
PHST- 2020/05/12 06:00 [pubmed]
PHST- 2020/08/14 06:00 [medline]
PST - ppublish
SO  - Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2020 Mar;36(3):198-204.