PMID- 32391112
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1792-1074 (Print)
IS  - 1792-1082 (Electronic)
IS  - 1792-1074 (Linking)
VI  - 19
IP  - 6
DP  - 2020 Jun
TI  - Detection of circulating tumor cells in patients with laryngeal cancer using 
      ScreenCell: Comparative pre- and post-operative analysis and association with 
      prognosis.
PG  - 4183-4188
LID - 10.3892/ol.2020.11528 [doi]
AB  - The presence of circulating tumor cells (CTCs) in the blood of patients with 
      metastatic breast, colorectal and prostate cancer have been widely investigated; 
      however, few studies have examined CTCs in patients with laryngeal cancer. The 
      present pilot study aimed to detect pre- and postoperative CTCs in the blood of 
      patients with laryngeal cancer and evaluate the association with prognosis. Eight 
      patients with laryngeal squamous cell carcinoma (LSCC) at stage III were included 
      in the present study and underwent total or subtotal laryngectomy and radical 
      bilateral neck lymph node dissection. Blood samples were collected from all 
      patients before and after surgery at different time-points. The following 
      processing steps were followed; preoperative blood sampling, surgery, 
      postoperative blood sampling at 3, 6 and 12 month follow-ups, and prognostic 
      association analysis. CTCs were retained on ScreenCell filters for cytological 
      characterization. The presence of CTCs was associated with a less favorable 
      prognosis, whereas a decrease of CTCs in the postoperative sampling was observed 
      in patients who exhibited an improved therapeutic response. The results of the 
      present pilot study revealed a possible association between the presence of CTCs 
      and a less favorable prognosis in patients with LSCC; therefore, these 
      preliminary findings may encourage further research into the incorporation of a 
      liquid biopsy in the management of LSCC, as this may help identify patients with 
      occult metastatic disease earlier and in a non-invasive manner. In addition, this 
      approach may represent novel independent prognostic factor for use in the 
      clinical evaluation of patients with LSCC.
CI  - Copyright (c) 2020, Spandidos Publications.
FAU - Rizzo, Maria Ida
AU  - Rizzo MI
AD  - Department of Surgical Science, Sapienza University of Rome, Rome 00186, Italy.
AD  - Craniofacial Center, Plastic and Maxillofacial Surgery Unit, Bambino Gesu 
      Children Hospital, Rome 00165, Italy.
FAU - Ralli, Massimo
AU  - Ralli M
AD  - Department of Sense Organs, Sapienza University of Rome, Rome 00186, Italy.
FAU - Nicolazzo, Chiara
AU  - Nicolazzo C
AD  - Department of Molecular Medicine-Circulating Tumor Cells Unit, Sapienza 
      University of Rome, Rome 00186, Italy.
FAU - Gradilone, Angela
AU  - Gradilone A
AD  - Department of Molecular Medicine-Circulating Tumor Cells Unit, Sapienza 
      University of Rome, Rome 00186, Italy.
FAU - Carletti, Raffaella
AU  - Carletti R
AD  - Department of Radiological, Oncological and Pathological Sciences, Sapienza 
      University of Rome, Rome 00186, Italy.
FAU - Di Gioia, Cira
AU  - Di Gioia C
AD  - Department of Radiological, Oncological and Pathological Sciences, Sapienza 
      University of Rome, Rome 00186, Italy.
FAU - De Vincentiis, Marco
AU  - De Vincentiis M
AD  - Department of Oral and Maxillofacial Sciences, Sapienza University of Rome, Rome 
      00186, Italy.
FAU - Greco, Antonio
AU  - Greco A
AD  - Department of Sense Organs, Sapienza University of Rome, Rome 00186, Italy.
LA  - eng
PT  - Journal Article
DEP - 20200410
PL  - Greece
TA  - Oncol Lett
JT  - Oncology letters
JID - 101531236
PMC - PMC7204636
OTO - NOTNLM
OT  - ScreenCell
OT  - circulating tumor cells
OT  - epithelial-mesenchymal transition
OT  - laryngeal cancer
OT  - liquid biopsy
EDAT- 2020/05/12 06:00
MHDA- 2020/05/12 06:01
PMCR- 2020/04/10
CRDT- 2020/05/12 06:00
PHST- 2019/08/10 00:00 [received]
PHST- 2020/02/20 00:00 [accepted]
PHST- 2020/05/12 06:00 [entrez]
PHST- 2020/05/12 06:00 [pubmed]
PHST- 2020/05/12 06:01 [medline]
PHST- 2020/04/10 00:00 [pmc-release]
AID - OL-0-0-11528 [pii]
AID - 10.3892/ol.2020.11528 [doi]
PST - ppublish
SO  - Oncol Lett. 2020 Jun;19(6):4183-4188. doi: 10.3892/ol.2020.11528. Epub 2020 Apr 
      10.