PMID- 32391616
OWN - NLM
STAT- MEDLINE
DCOM- 20210319
LR  - 20210319
IS  - 1365-313X (Electronic)
IS  - 0960-7412 (Linking)
VI  - 103
IP  - 4
DP  - 2020 Aug
TI  - LcEIL2/3 are involved in fruitlet abscission via activating genes related to 
      ethylene biosynthesis and cell wall remodeling in litchi.
PG  - 1338-1350
LID - 10.1111/tpj.14804 [doi]
AB  - Fruit crops are subject to precocious fruit abscission, during which the 
      phytohormone ethylene (ET) acts as a major positive regulator. However, the 
      molecular basis of ET-induced fruit abscission remains poorly understood. Here, 
      we show that two ETHYLENE INSENSITIVE 3-like (EIL) homologs in litchi, LcEIL2 and 
      LcEIL3, play a role in ET-activated fruitlet abscission. LcEIL2/3 were 
      significantly upregulated in the fruit abscission zone (AZ) during the ET-induced 
      fruitlet abscission in litchi. The presence of LcEIL2/3 in wild-type Arabidopsis 
      and ein3 eil1 mutants can accelerate the floral organ abscission. Moreover, the 
      electrophoretic mobility shift assay and dual luciferase reporter analysis 
      illustrated that LcEIL2/3 directly interacted with the gene promoters to activate 
      the expression of cell wall remodeling genes LcCEL2/8 and LcPG1/2, and ET 
      biosynthetic genes LcACS1/4/7 and LcACO2/3. Furthermore, we showed that LcPG1/2 
      were expressed in the floral abscission zone of Arabidopsis, and constitutive 
      expression of LcPG2 in Arabidopsis promoted the floral organ abscission. In 
      conclusion, we propose that LcEIL2/3 are involved in ET-induced fruitlet 
      abscission via controlling expression of genes related to ET biosynthesis and 
      cell wall remodeling in litchi.
CI  - (c) 2020 Society for Experimental Biology and John Wiley & Sons Ltd.
FAU - Ma, Xingshuai
AU  - Ma X
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Yuan, Ye
AU  - Yuan Y
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Wu, Qian
AU  - Wu Q
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Wang, Jun
AU  - Wang J
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Li, Jianguo
AU  - Li J
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, 510642, China.
FAU - Zhao, Minglei
AU  - Zhao M
AUID- ORCID: 0000-0002-2763-4180
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-Bioresources, South China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Litchi Engineering Research Center, College of Horticulture, South 
      China Agricultural University, Guangzhou, 510642, China.
AD  - Guangdong Provincial Key Laboratory of Postharvest Science of Fruits and 
      Vegetables, College of Horticulture, South China Agricultural University, 
      Guangzhou, 510642, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200610
PL  - England
TA  - Plant J
JT  - The Plant journal : for cell and molecular biology
JID - 9207397
RN  - 0 (Ethylenes)
RN  - 0 (Plant Growth Regulators)
RN  - 0 (Plant Proteins)
RN  - 0 (Transcription Factors)
RN  - 91GW059KN7 (ethylene)
SB  - IM
MH  - Arabidopsis
MH  - Cell Wall/*metabolism
MH  - Ethylenes/*biosynthesis
MH  - Flowers/metabolism/physiology
MH  - Fruit/*metabolism/physiology
MH  - *Genes, Plant/physiology
MH  - Litchi/*metabolism
MH  - Phylogeny
MH  - Plant Growth Regulators/*biosynthesis
MH  - Plant Proteins/metabolism/*physiology
MH  - Plants, Genetically Modified
MH  - Transcription Factors/metabolism/*physiology
OTO - NOTNLM
OT  - LcEIL2/3
OT  - cell wall remodeling
OT  - ethylene biosynthesis
OT  - fruitlet abscission
OT  - litchi (Litchi chinensis Sonn.)
EDAT- 2020/05/12 06:00
MHDA- 2021/03/20 06:00
CRDT- 2020/05/12 06:00
PHST- 2019/12/03 00:00 [received]
PHST- 2020/04/07 00:00 [revised]
PHST- 2020/04/28 00:00 [accepted]
PHST- 2020/05/12 06:00 [pubmed]
PHST- 2021/03/20 06:00 [medline]
PHST- 2020/05/12 06:00 [entrez]
AID - 10.1111/tpj.14804 [doi]
PST - ppublish
SO  - Plant J. 2020 Aug;103(4):1338-1350. doi: 10.1111/tpj.14804. Epub 2020 Jun 10.