PMID- 3239416
OWN - NLM
STAT- MEDLINE
DCOM- 19890510
LR  - 20151119
IS  - 0001-6772 (Print)
IS  - 0001-6772 (Linking)
VI  - 134
IP  - 1
DP  - 1988 Sep
TI  - Repeated measurements of cerebral blood flow in rats. Comparisons between the 
      hydrogen clearance method and laser Doppler flowmetry.
PG  - 133-42
AB  - Changes in cerebral blood flow (CBF), in chloralose-anaesthetized spontaneously 
      hypertensive rats, were measured simultaneously with the hydrogen clearance (HC) 
      method and laser Doppler flowmetry (LDFM) in order to examine the correlation 
      between results obtained with the two techniques. To induce changes in CBF the 
      rats were bled and CBF measured at different levels of mean arterial pressure. As 
      would be expected, with the platinum electrode at the depth of about 1 mm in the 
      somatosensory cortex, HC gave biexponential curves reflecting clearance in two 
      distinct compartments. During control situation the HC method gave the following 
      values (mean +/- SE, n = 20, ml min-1 100 g-1): fast (fCBF) 158.6 +/- 11.5, slow 
      (sCBF) 29.1 +/- 1.6 with a weighted mean flow (mCBF) of 83.3 +/- 7.4. These fCBF 
      and sCBF values correspond well to those of others obtained with 
      [14C]iodoantipyrine autoradiography in the cerebral cortex and corpus callosum, 
      and better than the results of other studies using HC in rats. Our results, 
      comparing data from HC and LDFM, show a linear relationship between relative 
      values of blood flow changes, the coefficients being 0.658, 0.876 and 0.878 for 
      the correlation between the LDFM data and relative changes in fCBF, sCBF and 
      mCBF, respectively. All three regression lines were significantly different from 
      the line of identity. Much of the discrepancy between the two methods may be 
      related to limitations inherent in each of them, despite efforts to minimize 
      their effects. Thus the depth sensitivity of LDFM in the brain may be greater 
      than expected. In conclusion, the laser Doppler method seems, nevertheless, to be 
      most useful for continuous estimations of changes in cerebral blood flow.
FAU - Skarphedinsson, J O
AU  - Skarphedinsson JO
AD  - Department of Physiology, University of Goteborg, Sweden.
FAU - Harding, H
AU  - Harding H
FAU - Thoren, P
AU  - Thoren P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Acta Physiol Scand
JT  - Acta physiologica Scandinavica
JID - 0370362
RN  - 7YNJ3PO35Z (Hydrogen)
SB  - IM
MH  - Animals
MH  - Autoradiography
MH  - *Cerebrovascular Circulation
MH  - Hydrogen/pharmacokinetics
MH  - Lasers
MH  - Methods
MH  - Rats
MH  - Rats, Inbred SHR
EDAT- 1988/09/01 00:00
MHDA- 1988/09/01 00:01
CRDT- 1988/09/01 00:00
PHST- 1988/09/01 00:00 [pubmed]
PHST- 1988/09/01 00:01 [medline]
PHST- 1988/09/01 00:00 [entrez]
AID - 10.1111/j.1748-1716.1988.tb08469.x [doi]
PST - ppublish
SO  - Acta Physiol Scand. 1988 Sep;134(1):133-42. doi: 
      10.1111/j.1748-1716.1988.tb08469.x.