PMID- 32395704
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240328
IS  - 2573-9832 (Electronic)
IS  - 2573-9832 (Linking)
VI  - 2
IP  - 5
DP  - 2020 May
TI  - Differential expression of the five redox complexes in the retinal mitochondria 
      or rod outer segment disks is consistent with their different functionality.
PG  - 315-324
LID - 10.1096/fba.2019-00093 [doi]
AB  - PURPOSE: The retinal rod outer segment (OS) disk membranes, devoid of 
      mitochondria, conducts oxidative phosphorylation (OxPhos). This study aimed at 
      identifying which proteins expressed in the retinal rod OS disks determined the 
      considerable adenosine-5'-triphosphate production and oxygen consumption observed 
      in comparison with retinal mitochondria. PROCEDURES: Characterization was 
      conducted by immunogold transmission electron microscopy on retinal sections. 
      OxPhos was studied by oximetry and luminometry. The proteomes of OS disks and 
      mitochondria purified from bovine retinas were studied by mass spectrometry. 
      Statistical and bioinformatic analyses were conducted by univariate, 
      multivariate, and machine learning methods. RESULTS: Weighted gene coexpression 
      network analysis identified two protein expression profile modules functionally 
      associated with either retinal mitochondria or disk samples, in function of a 
      strikingly different ability of each sample to utilized diverse substrate for 
      F(1)F(o)-ATP synthase. The OS disk proteins correlated better than mitochondria 
      with the tricarboxylic acids cycle and OxPhos proteins. CONCLUSIONS: The 
      differential enrichment of the expression profile of the OxPhos proteins in the 
      disks versus mitochondria suggests that these proteins may represent a true 
      proteome component of the former, with different functionality. These findings 
      may shed new light on the pathogenesis of rod-driven retinal degenerative 
      diseases.
CI  - (c)2020 Universita de Genova-DIFAR.
FAU - Bruschi, Maurizio
AU  - Bruschi M
AD  - Laboratory of Molecular Nephrology Istituto Giannina Gaslini Genoa Italy.
FAU - Bartolucci, Martina
AU  - Bartolucci M
AD  - Laboratory of Mass Spectrometry-Core Facilities Istituto Giannina Gaslini Genova 
      Italy.
FAU - Petretto, Andrea
AU  - Petretto A
AD  - Laboratory of Mass Spectrometry-Core Facilities Istituto Giannina Gaslini Genova 
      Italy.
FAU - Calzia, Daniela
AU  - Calzia D
AD  - Dipartimento di Farmacia-DIFAR Universita di Genova Genoa Italy.
FAU - Caicci, Federico
AU  - Caicci F
AD  - Department of Biology Universita di Padova Padova Italy.
FAU - Manni, Lucia
AU  - Manni L
AD  - Department of Biology Universita di Padova Padova Italy.
FAU - Traverso, Carlo Enrico
AU  - Traverso CE
AD  - Clinica Oculistica, (Di.N.O.G.M.I.) Universita Department of Intensive Care di 
      Genova IRCCS Azienda Ospedaliera Universitaria San Martino-IST Genoa Italy.
FAU - Candiano, Giovanni
AU  - Candiano G
AD  - Laboratory of Molecular Nephrology Istituto Giannina Gaslini Genoa Italy.
FAU - Panfoli, Isabella
AU  - Panfoli I
AD  - Dipartimento di Farmacia-DIFAR Universita di Genova Genoa Italy.
LA  - eng
PT  - Journal Article
DEP - 20200331
PL  - United States
TA  - FASEB Bioadv
JT  - FASEB bioAdvances
JID - 101733210
PMC - PMC7211042
OTO - NOTNLM
OT  - F1Fo ATP synthase
OT  - Orbitrap Velos
OT  - aerobic metabolism
OT  - oxidative phosphorylation
OT  - rod outer segment
COIS- The authors declare that they have no competing interests.
EDAT- 2020/05/13 06:00
MHDA- 2020/05/13 06:01
PMCR- 2020/03/31
CRDT- 2020/05/13 06:00
PHST- 2019/11/27 00:00 [received]
PHST- 2019/11/27 00:00 [revised]
PHST- 2020/03/05 00:00 [accepted]
PHST- 2020/05/13 06:00 [entrez]
PHST- 2020/05/13 06:00 [pubmed]
PHST- 2020/05/13 06:01 [medline]
PHST- 2020/03/31 00:00 [pmc-release]
AID - FBA21123 [pii]
AID - 10.1096/fba.2019-00093 [doi]
PST - epublish
SO  - FASEB Bioadv. 2020 Mar 31;2(5):315-324. doi: 10.1096/fba.2019-00093. eCollection 
      2020 May.