PMID- 32396897
OWN - NLM
STAT- MEDLINE
DCOM- 20200914
LR  - 20210514
IS  - 1423-0135 (Electronic)
IS  - 1018-1172 (Print)
IS  - 1018-1172 (Linking)
VI  - 57
IP  - 4
DP  - 2020
TI  - A Novel Model of Balloon Angioplasty Injury in Rat Arteriovenous Fistula.
PG  - 223-235
LID - 10.1159/000507080 [doi]
AB  - BACKGROUND: There are very few animal models of balloon angioplasty injury in 
      arteriovenous fistula (AVF), hindering insight into the pathophysiologic 
      processes following angioplasty in AVF. The objective of the study was to develop 
      and characterize a rat model of AVF angioplasty injury. METHODS: Balloon 
      angioplasty in 12- to 16-week-old Sprague-Dawley rats was performed at the 
      arteriovenous anastomosis 14 days post-AVF creation with a 2F Fogarty balloon 
      catheter. Morphometry and protein expression of endothelial nitric oxide synthase 
      (eNOS), monocyte-chemoattractant protein-1 (MCP-1), alpha-smooth muscle actin 
      (alpha-SMA), CD68 (macrophage marker), and collagen expression in AVFs with and 
      without angioplasty were assessed. RESULTS: In AVFs with angioplasty versus 
      without angioplasty: (1) angioplasty increased AVF-vein and artery intimal 
      hyperplasia, (2) angioplasty decreased eNOS protein expression in AVF-vein and 
      artery at 21 days post-AVF creation and remained decreased in the AVF-vein 
      angioplasty group at 35 days, (3) angioplasty increased AVF-vein and artery alpha-SMA 
      expression within the intimal region at 35 days, (4) angioplasty increased the 
      expression of AVF-vein MCP-1 at 21 days and CD68 at 21 and 35 days, and (5) 
      angioplasty increased AVF-vein and artery collagen expression at 35 days. 
      CONCLUSION: Our findings describe a reproducible rat model to better understand 
      the pathophysiologic mechanisms that ensue following AVF angioplasty.
CI  - (c) 2020 S. Karger AG, Basel.
FAU - Somarathna, Maheshika
AU  - Somarathna M
AD  - Department of Medicine and Division of Nephrology, Division of Nephrology, 
      Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, 
      USA.
FAU - Isayeva-Waldrop, Tatyana
AU  - Isayeva-Waldrop T
AD  - Department of Medicine and Division of Nephrology, Division of Nephrology, 
      Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, 
      USA.
FAU - Al-Balas, Alian
AU  - Al-Balas A
AD  - Department of Medicine and Division of Nephrology, Division of Nephrology, 
      Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, 
      USA.
FAU - Guo, Lingling
AU  - Guo L
AD  - Department of Medicine and Division of Nephrology, Division of Nephrology, 
      Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, 
      USA.
FAU - Lee, Timmy
AU  - Lee T
AD  - Department of Medicine and Division of Nephrology, Division of Nephrology, 
      Department of Medicine, University of Alabama at Birmingham, Birmingham, Alabama, 
      USA, txlee@uab.edu.
AD  - Veterans Affairs Medical Center, Section of Nephrology, Birmingham, Alabama, USA, 
      txlee@uab.edu.
LA  - eng
GR  - R44 DK109789/DK/NIDDK NIH HHS/United States
GR  - T32 DK116672/DK/NIDDK NIH HHS/United States
GR  - P30 DK079337/DK/NIDDK NIH HHS/United States
GR  - I01 BX003387/BX/BLRD VA/United States
GR  - R01 HL139692/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20200512
PL  - Switzerland
TA  - J Vasc Res
JT  - Journal of vascular research
JID - 9206092
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD68 protein, rat)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (smooth muscle actin, rat)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
RN  - EC 1.14.13.39 (Nos3 protein, rat)
SB  - IM
MH  - Actins/metabolism
MH  - *Angioplasty, Balloon
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation, Myelomonocytic/metabolism
MH  - *Arteriovenous Shunt, Surgical
MH  - Chemokine CCL2/metabolism
MH  - Collagen/metabolism
MH  - Disease Models, Animal
MH  - Femoral Artery/*injuries/metabolism/pathology/surgery
MH  - Femoral Vein/*injuries/metabolism/pathology/surgery
MH  - Male
MH  - Neointima
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - *Vascular Remodeling
MH  - Vascular System Injuries/*etiology/metabolism/pathology
PMC - PMC7381362
MID - NIHMS1580794
OTO - NOTNLM
OT  - Angioplasty
OT  - Arteriovenous fistula
OT  - Intimal hyperplasia
EDAT- 2020/05/13 06:00
MHDA- 2020/09/15 06:00
PMCR- 2021/05/12
CRDT- 2020/05/13 06:00
PHST- 2019/05/20 00:00 [received]
PHST- 2020/03/09 00:00 [accepted]
PHST- 2020/05/13 06:00 [pubmed]
PHST- 2020/09/15 06:00 [medline]
PHST- 2020/05/13 06:00 [entrez]
PHST- 2021/05/12 00:00 [pmc-release]
AID - 000507080 [pii]
AID - 10.1159/000507080 [doi]
PST - ppublish
SO  - J Vasc Res. 2020;57(4):223-235. doi: 10.1159/000507080. Epub 2020 May 12.