PMID- 32399929
OWN - NLM
STAT- MEDLINE
DCOM- 20210311
LR  - 20210311
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2137
DP  - 2020
TI  - Evaluation of the Immune Regulatory Properties of Dendritic Cells During Fasciola 
      hepatica Infection.
PG  - 181-190
LID - 10.1007/978-1-0716-0475-5_13 [doi]
AB  - Dendritic cells (DCs) are potent antigen-presenting cells that possess the 
      ability to stimulate naive T cells, initiating the adaptive immune response. Ex 
      vivo DC cultures are useful to evaluate how helminths regulate DC maturation and 
      stimulatory activity. Here, we describe how to isolate CD11c(+) from F. 
      hepatica-infected mice to evaluate their activation state, cytokine production 
      and regulatory function in an allogeneic T cell assay.
FAU - Freire, Teresa
AU  - Freire T
AD  - Grupo de Inmunomodulacion y Desarrollo de Vacunas, Departamento de 
      Inmunobiologia, Facultad de Medicina, Universidad de La Republica, Montevideo, 
      Uruguay. tfreire@fmed.edu.uy.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, Helminth)
RN  - 0 (CD11c Antigen)
RN  - 0 (Immunologic Factors)
SB  - IM
MH  - Animals
MH  - Antigens, Helminth/immunology
MH  - CD11c Antigen/immunology
MH  - Dendritic Cells/*immunology/*parasitology
MH  - Fasciola hepatica/*immunology
MH  - Fascioliasis/*immunology/parasitology
MH  - Female
MH  - Immunologic Factors/immunology
MH  - Lymphocyte Activation/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - T-Lymphocytes/immunology/parasitology
OTO - NOTNLM
OT  - CD11c
OT  - Cytokines
OT  - Dendritic cell
OT  - Fasciola hepatica
OT  - Immune regulation
OT  - Maturation
OT  - Mixed lymphocyte reaction
OT  - T cell proliferation
EDAT- 2020/05/14 06:00
MHDA- 2021/03/12 06:00
CRDT- 2020/05/14 06:00
PHST- 2020/05/14 06:00 [entrez]
PHST- 2020/05/14 06:00 [pubmed]
PHST- 2021/03/12 06:00 [medline]
AID - 10.1007/978-1-0716-0475-5_13 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2137:181-190. doi: 10.1007/978-1-0716-0475-5_13.