PMID- 32402349
OWN - NLM
STAT- MEDLINE
DCOM- 20201230
LR  - 20201230
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 244
DP  - 2020 May
TI  - A porcine alveolar macrophage cell line stably expressing CD163 demonstrates 
      virus replication and cytokine secretion characteristics similar to primary 
      alveolar macrophages following PRRSV infection.
PG  - 108690
LID - S0378-1135(19)31437-3 [pii]
LID - 10.1016/j.vetmic.2020.108690 [doi]
AB  - The in vitro investigation of cytokine secretion induced by porcine reproductive 
      and respiratory syndrome virus (PRRSV) requires porcine alveolar macrophages 
      (PAMs) and their interaction with immunocytes. However, immortalized monoclonal 
      PAMs (mPAMs) are non-permissive for PRRSV infection. The porcine CD163 receptor 
      isolated from primary PAMs (pPAMs) confers susceptibility to PRRSV infection; 
      thus, this approach could be used to establish a novel cell line to facilitate 
      the exploration of PRRSV infection kinetics. Here, we amplified the coding region 
      of the CD163 gene from pPAMs and integrated it into an mPAM line using a 
      lentivirus expression system. After verification, the monoclonal PAM cell line 
      stably expressing CD163 (mPAM-CD163-GFP) was infected with either the highly 
      pathogenic PRRSV strain JXA1 or the classical PRRSV strain SD1, which produced 
      high infectious titers of progeny virus reaching > 10(9) copies/mL or a 50 % 
      tissue culture infective dose of 10(5.5) over at least 100 cell generations. We 
      also investigated cytokine and Toll-like receptor expression in infected 
      mPAM-CD163-GFP cells and pPAMs. The mPAM-CD163-GFP cell line showed similar 
      patterns of viral replication and cytokine secretion compared with pPAMs, so it 
      may be extremely useful for replacing primary cells for in vitro investigations 
      of the mechanisms of cytokine secretion and interactions between PRRSV-infected 
      PAMs and immunocytes.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Xu, Yu-Lin
AU  - Xu YL
AD  - College of Veterinary Medicine, Shandong Agricultural University, and Shandong 
      Provincial Key Laboratory of Animal Biotechnology and Disease Control and 
      Prevention, Taian 271000, China; East China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture and Rural Affairs of China, Taian 
      271000, China.
FAU - Wu, Shao-Peng
AU  - Wu SP
AD  - College of Veterinary Medicine, Shandong Agricultural University, and Shandong 
      Provincial Key Laboratory of Animal Biotechnology and Disease Control and 
      Prevention, Taian 271000, China; East China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture and Rural Affairs of China, Taian 
      271000, China.
FAU - Li, Yun-Gang
AU  - Li YG
AD  - Shandong Centers for Animal Disease Control and Prevention, Jinan 250022, China.
FAU - Sun, Feng-Xia
AU  - Sun FX
AD  - College of Resources and Environment, Shandong Agricultural University, Taian 
      271000, China.
FAU - Wang, Qiu-Ju
AU  - Wang QJ
AD  - College of Veterinary Medicine, Shandong Agricultural University, and Shandong 
      Provincial Key Laboratory of Animal Biotechnology and Disease Control and 
      Prevention, Taian 271000, China; East China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture and Rural Affairs of China, Taian 
      271000, China.
FAU - Zhao, Qing
AU  - Zhao Q
AD  - College of Veterinary Medicine, Shandong Agricultural University, and Shandong 
      Provincial Key Laboratory of Animal Biotechnology and Disease Control and 
      Prevention, Taian 271000, China; East China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture and Rural Affairs of China, Taian 
      271000, China.
FAU - Yu, Jiang
AU  - Yu J
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Jinan 251000, China; Shandong Key Laboratory of Animal 
      Disease Control and Breeding, Jinan 250100, China.
FAU - Tian, Fu-Lin
AU  - Tian FL
AD  - Shandong Centers for Animal Disease Control and Prevention, Jinan 250022, China.
FAU - Wu, Jia-Qiang
AU  - Wu JQ
AD  - Institute of Animal Science and Veterinary Medicine, Shandong Academy of 
      Agricultural Sciences, Jinan 251000, China; Shandong Key Laboratory of Animal 
      Disease Control and Breeding, Jinan 250100, China. Electronic address: 
      wujiaqiang2000@sina.com.
FAU - Zhu, Rui-Liang
AU  - Zhu RL
AD  - College of Veterinary Medicine, Shandong Agricultural University, and Shandong 
      Provincial Key Laboratory of Animal Biotechnology and Disease Control and 
      Prevention, Taian 271000, China; East China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture and Rural Affairs of China, Taian 
      271000, China. Electronic address: zhurl@sdau.edu.cn.
FAU - Peng, Jun
AU  - Peng J
AD  - College of Veterinary Medicine, Shandong Agricultural University, and Shandong 
      Provincial Key Laboratory of Animal Biotechnology and Disease Control and 
      Prevention, Taian 271000, China; East China Research Center of Animal Epidemic 
      Pathogen Biology, Ministry of Agriculture and Rural Affairs of China, Taian 
      271000, China. Electronic address: jpeng@sdau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200417
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/*genetics
MH  - Antigens, Differentiation, Myelomonocytic/*genetics
MH  - Cell Line
MH  - Cells, Cultured
MH  - Cytokines/*immunology
MH  - Lentivirus/genetics
MH  - Macrophages, Alveolar/*immunology/*virology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Receptors, Cell Surface/*genetics
MH  - Swine
MH  - Virus Cultivation
MH  - *Virus Replication
OTO - NOTNLM
OT  - CD163
OT  - Cytokine
OT  - Porcine alveolar macrophage cell line
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Secretion characteristics
OT  - Stable expression
COIS- Declaration of Competing Interest The authors declare that they have no competing 
      interests.
EDAT- 2020/05/14 06:00
MHDA- 2020/12/31 06:00
CRDT- 2020/05/14 06:00
PHST- 2019/12/11 00:00 [received]
PHST- 2020/04/04 00:00 [revised]
PHST- 2020/04/10 00:00 [accepted]
PHST- 2020/05/14 06:00 [entrez]
PHST- 2020/05/14 06:00 [pubmed]
PHST- 2020/12/31 06:00 [medline]
AID - S0378-1135(19)31437-3 [pii]
AID - 10.1016/j.vetmic.2020.108690 [doi]
PST - ppublish
SO  - Vet Microbiol. 2020 May;244:108690. doi: 10.1016/j.vetmic.2020.108690. Epub 2020 
      Apr 17.