PMID- 32402921
OWN - NLM
STAT- MEDLINE
DCOM- 20210621
LR  - 20220531
IS  - 1872-8332 (Electronic)
IS  - 0169-5002 (Linking)
VI  - 145
DP  - 2020 Jul
TI  - Programmed death-ligand 1 expression profiling in thymic epithelial cell tumors: 
      Clinicopathological features and quantitative digital image analyses.
PG  - 40-47
LID - S0169-5002(20)30408-6 [pii]
LID - 10.1016/j.lungcan.2020.04.038 [doi]
AB  - OBJECTIVES: Whether the extent of expression of programmed death-ligand 1 (PD-L1) 
      is clinically significant remains uncertain, although immuno-oncological features 
      have been studied in thymic epithelial cell tumors (TETs). We investigated the 
      histological features of PD-L1 expression in TETs, and assessed PD-L1 expression 
      using digital image analysis. MATERIALS AND METHODS: Participants comprised 66 
      patients with TET who underwent surgical resection between 2001 and 2016. We 
      calculated tumor cell-positive ratio as total proportion score (TPS) with 
      immunohistochemistry using SP263 anti-PD-L1 monoclonal antibody. PD-L1 expression 
      was also quantified using digital image analysis of whole-slide images. We 
      evaluated the relationship between conventional visual TPS using optical 
      microscopy (TPS-V) and TPS from digital image analysis (TPS-IA). We further 
      classified all TETs into high or low PD-L1 expression groups and assessed the 
      clinical significance of PD-L1 expression level using TPS-V and TPS-IA. RESULTS: 
      WHO histological types were Type A (n = 8), AB (n = 18), B1 (n = 5), B2 (n = 16), 
      B3 (n = 6), metaplastic thymoma (n = 2), and thymic carcinoma (TC) (n = 11). 
      Median TPS-Vs were 2%, 2%, 10 %, 65 %, 90 %, 1%, and 20 %, respectively. TPS-IAs 
      correlated with TPS-Vs in TETs overall and in thymomas, but not in TCs. PD-L1 
      expression levels in TETs differed significantly among histological types. 
      Whether TPS-V or TPS-IA were used, the PD-L1(high) group included more cases of 
      the more aggressive histological types. Recurrence-free survival (RFS) was 
      shorter in the PD-L1(high) group than in the PD-L1(low) group in thymoma using 
      TPS-IA, whereas RFS of the PD-L1(high) group was shorter in all TETs using TPS-V. 
      CONCLUSION: PD-L1 expression levels depended on the histological type of TET. 
      Extensive PD-L1 expression in TETs was associated with poor prognosis. Digital 
      image analysis is feasible for evaluating PD-L1 expression in TETs and might 
      offer clinically relevant features of thymomas.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Ishihara, Shunta
AU  - Ishihara S
AD  - Division of Thoracic Surgery, Department of Surgery, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Okada, Satoru
AU  - Okada S
AD  - Division of Thoracic Surgery, Department of Surgery, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Ogi, Hiroshi
AU  - Ogi H
AD  - Department of Pathology and Applied Neurobiology, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Kodama, Yoshinori
AU  - Kodama Y
AD  - Department of Pathology and Applied Neurobiology, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Shimomura, Masanori
AU  - Shimomura M
AD  - Division of Thoracic Surgery, Department of Surgery, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Tsunezuka, Hiroaki
AU  - Tsunezuka H
AD  - Division of Thoracic Surgery, Department of Surgery, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Itoh, Kyoko
AU  - Itoh K
AD  - Department of Pathology and Applied Neurobiology, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan.
FAU - Marx, Alexander
AU  - Marx A
AD  - Institute of Pathology, University Medical Centre Mannheim, Heidelberg 
      University, Mannheim, Germany.
FAU - Inoue, Masayoshi
AU  - Inoue M
AD  - Division of Thoracic Surgery, Department of Surgery, Graduate School of Medical 
      Science, Kyoto Prefectural University of Medicine, 465 Kajii-cho, Kamigyo-ku, 
      Kyoto 602-8566, Japan. Electronic address: mainoue@koto.kpu-m.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200506
PL  - Ireland
TA  - Lung Cancer
JT  - Lung cancer (Amsterdam, Netherlands)
JID - 8800805
RN  - 0 (B7-H1 Antigen)
RN  - 0 (CD274 protein, human)
SB  - IM
MH  - B7-H1 Antigen
MH  - Epithelial Cells
MH  - Humans
MH  - *Lung Neoplasms
MH  - *Thymoma
MH  - *Thymus Neoplasms
OTO - NOTNLM
OT  - Digital image analysis
OT  - Programmed cell death ligand 1
OT  - Thymic epithelial tumor
OT  - Thymoma
OT  - Whole-Slide imaging
COIS- Declaration of Competing Interest The authors have no conflicts of interest.
EDAT- 2020/05/14 06:00
MHDA- 2021/06/22 06:00
CRDT- 2020/05/14 06:00
PHST- 2019/12/02 00:00 [received]
PHST- 2020/04/26 00:00 [revised]
PHST- 2020/04/28 00:00 [accepted]
PHST- 2020/05/14 06:00 [pubmed]
PHST- 2021/06/22 06:00 [medline]
PHST- 2020/05/14 06:00 [entrez]
AID - S0169-5002(20)30408-6 [pii]
AID - 10.1016/j.lungcan.2020.04.038 [doi]
PST - ppublish
SO  - Lung Cancer. 2020 Jul;145:40-47. doi: 10.1016/j.lungcan.2020.04.038. Epub 2020 
      May 6.