PMID- 32404209
OWN - NLM
STAT- MEDLINE
DCOM- 20210105
LR  - 20210105
IS  - 1297-9716 (Electronic)
IS  - 0928-4249 (Print)
IS  - 0928-4249 (Linking)
VI  - 51
IP  - 1
DP  - 2020 May 13
TI  - Evaluation of local and systemic immune responses in pigs experimentally 
      challenged with porcine reproductive and respiratory syndrome virus.
PG  - 66
LID - 10.1186/s13567-020-00789-7 [doi]
LID - 66
AB  - The host-associated defence system responsible for the clearance of porcine 
      reproductive and respiratory syndrome virus (PRRSV) from infected pigs is 
      currently poorly understood. To better understand the dynamics of host-pathogen 
      interactions, seventy-five of 100 pigs infected with PRRSV-JA142 and 25 control 
      pigs were euthanized at 3, 10, 21, 28 and 35 days post-challenge (dpc). Blood, 
      lung, bronchoalveolar lavage (BAL) and bronchial lymph node (BLN) samples were 
      collected to evaluate the cellular immune responses. The humoral responses were 
      evaluated by measuring the levels of anti-PRRSV IgG and serum virus-neutralizing 
      (SVN) antibodies. Consequently, the highest viral loads in the sera and lungs of 
      the infected pigs were detected between 3 and 10 dpc, and these resulted in 
      moderate to mild interstitial pneumonia, which resolved accompanied by the 
      clearance of most of the virus by 28 dpc. At peak viremia, the frequencies of 
      alveolar macrophages in infected pigs were significantly decreased, whereas the 
      monocyte-derived DC/macrophage and conventional DC frequencies were increased, 
      and these effects coincided with the early induction of local T-cell responses 
      and the presence of proinflammatory cytokines/chemokines in the lungs, BAL, and 
      BLN as early as 10 dpc. Conversely, the systemic T-cell responses measured in the 
      peripheral blood mononuclear cells were delayed and significantly induced only 
      after the peak viremic stage between 3 and 10 dpc. Taken together, our results 
      suggest that activation of immune responses in the lung could be the key elements 
      for restraining PRRSV through the early induction of T-cell responses at the 
      sites of virus replication.
FAU - Nazki, Salik
AU  - Nazki S
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Khatun, Amina
AU  - Khatun A
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
AD  - Department of Pathology, Faculty of Animal Science and Veterinary Medicine, 
      Sher-e-Bangla Agricultural University, Dhaka, 1207, Bangladesh.
FAU - Jeong, Chang-Gi
AU  - Jeong CG
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Mattoo, Sameer Ul Salam
AU  - Mattoo SUS
AD  - Division of Biotechnology, Advanced Institute of Environment and Bioscience, 
      College of Environmental & Biosource Science, Jeonbuk National University, Iksan, 
      South Korea.
FAU - Gu, Suna
AU  - Gu S
AD  - Division of Biotechnology, Advanced Institute of Environment and Bioscience, 
      College of Environmental & Biosource Science, Jeonbuk National University, Iksan, 
      South Korea.
FAU - Lee, Sim-In
AU  - Lee SI
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Kim, Seung-Chai
AU  - Kim SC
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Park, Ji-Hyo
AU  - Park JH
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Yang, Myoun-Sik
AU  - Yang MS
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Kim, Bumseok
AU  - Kim B
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea.
FAU - Park, Choi-Kyu
AU  - Park CK
AD  - College of Veterinary Medicine, Kyungpook National University, Daegu, South 
      Korea.
FAU - Lee, Sang-Myeong
AU  - Lee SM
AD  - Division of Biotechnology, Advanced Institute of Environment and Bioscience, 
      College of Environmental & Biosource Science, Jeonbuk National University, Iksan, 
      South Korea. leesangm@jbnu.ac.kr.
FAU - Kim, Won-Il
AU  - Kim WI
AUID- ORCID: 0000-0002-0465-0794
AD  - College of Veterinary Medicine, Jeonbuk National University, Iksan, South Korea. 
      kwi0621@jbnu.ac.kr.
LA  - eng
GR  - PJ01181601/Ministry of Food, Agriculture, Forestry and Fisheries in the Republic 
      of Korea/
GR  - PJ01261201/Ministry of Food, Agriculture, Forestry and Fisheries in the Republic 
      of Korea/
GR  - 315029-3/Ministry of Food, Agriculture, Forestry and Fisheries in the Republic of 
      Korea/
PT  - Journal Article
DEP - 20200513
PL  - England
TA  - Vet Res
JT  - Veterinary research
JID - 9309551
SB  - IM
MH  - *Adaptive Immunity
MH  - Animals
MH  - Bronchi/immunology/virology
MH  - Bronchoalveolar Lavage Fluid/*immunology/virology
MH  - *Immunity, Innate
MH  - Lung/*immunology/virology
MH  - Lymph Nodes/*immunology/virology
MH  - Parenchymal Tissue/immunology/virology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*physiology
MH  - Sus scrofa
MH  - Swine
PMC - PMC7222343
COIS- The authors declare that they have no competing interests.
EDAT- 2020/05/15 06:00
MHDA- 2021/01/06 06:00
PMCR- 2020/05/13
CRDT- 2020/05/15 06:00
PHST- 2019/12/01 00:00 [received]
PHST- 2020/03/26 00:00 [accepted]
PHST- 2020/05/15 06:00 [entrez]
PHST- 2020/05/15 06:00 [pubmed]
PHST- 2021/01/06 06:00 [medline]
PHST- 2020/05/13 00:00 [pmc-release]
AID - 10.1186/s13567-020-00789-7 [pii]
AID - 789 [pii]
AID - 10.1186/s13567-020-00789-7 [doi]
PST - epublish
SO  - Vet Res. 2020 May 13;51(1):66. doi: 10.1186/s13567-020-00789-7.