PMID- 32404543
OWN - NLM
STAT- MEDLINE
DCOM- 20210331
LR  - 20210331
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 43
IP  - 7
DP  - 2020 Jul 1
TI  - Latifolin Inhibits Oxidative Stress-Induced Senescence via Upregulation of SIRT1 
      in Human Dermal Fibroblasts.
PG  - 1104-1110
LID - 10.1248/bpb.b20-00094 [doi]
AB  - Latifolin, a natural flavonoid found in Dalbergia odorifera T. Chen, has been 
      reported to exhibit anti-inflammatory and anticarcinogenic activities in vitro. 
      However, the anti-aging effects of latifolin are unknown. In this study, we 
      selected a model in vitro system, hydrogen peroxide (H(2)O(2))-induced senescence 
      in human dermal fibroblasts (HDFs), to examine the protective effects of 
      latifolin against senescence and the detailed molecular mechanisms involved. 
      Latifolin reversed the senescence-like phenotypes of the oxidant-challenged 
      model, including senescence-associated beta-galactosidase (SA-beta-gal) staining, cell 
      proliferation, and the expression of senescence-related proteins, such as 
      caveolin-1, ac-p53, p21(Cip1/WAF1), p16(Ink4alpha), pRb, and cyclinD1. We also found 
      that latifolin induced the expression of silent information regulator 1 (SIRT1) 
      in a concentration- and time-dependent manner, and the anti-senescence effect of 
      latifolin was abrogated by SIRT1 inhibition. Latifolin also suppressed the 
      activation of Akt and S6K1 and attenuated the increase in SA-beta-gal activity after 
      H(2)O(2) exposure. Our results indicate that latifolin exerts protective effects 
      against senescence in HDFs and that induction of SIRT1 and inhibition of the 
      mammalian target of rapamycin (mTOR) pathway are key mediators of its anti-aging 
      effects.
FAU - Lim, Seok-Hee
AU  - Lim SH
AD  - Department of Biochemistry, Wonkwang University School of Medicine.
FAU - Li, Bing Si
AU  - Li BS
AD  - Department of Biochemistry, Wonkwang University School of Medicine.
FAU - Zhu, Ri Zhe
AU  - Zhu RZ
AD  - Department of Biochemistry, Wonkwang University School of Medicine.
FAU - Seo, Jae-Ho
AU  - Seo JH
AD  - Department of Biochemistry, Wonkwang University School of Medicine.
FAU - Choi, Byung-Min
AU  - Choi BM
AD  - Department of Biochemistry, Wonkwang University School of Medicine.
LA  - eng
PT  - Journal Article
DEP - 20200512
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Phenols)
RN  - 0 (latifolin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 3.5.1.- (SIRT1 protein, human)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Cells, Cultured
MH  - Cellular Senescence/*drug effects/physiology
MH  - Dose-Response Relationship, Drug
MH  - Fibroblasts/*drug effects/metabolism
MH  - Humans
MH  - Hydrogen Peroxide/toxicity
MH  - Oxidative Stress/*drug effects/physiology
MH  - Phenols/*pharmacology
MH  - Sirtuin 1/*biosynthesis
MH  - Skin/cytology/drug effects/metabolism
MH  - Up-Regulation/*drug effects/physiology
OTO - NOTNLM
OT  - human dermal fibroblast
OT  - latifolin
OT  - mammalian target of rapamycin
OT  - oxidative stress
OT  - senescence
OT  - silent information regulator 1
EDAT- 2020/05/15 06:00
MHDA- 2021/04/01 06:00
CRDT- 2020/05/15 06:00
PHST- 2020/05/15 06:00 [pubmed]
PHST- 2021/04/01 06:00 [medline]
PHST- 2020/05/15 06:00 [entrez]
AID - 10.1248/bpb.b20-00094 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2020 Jul 1;43(7):1104-1110. doi: 10.1248/bpb.b20-00094. Epub 
      2020 May 12.