PMID- 32407430
OWN - NLM
STAT- MEDLINE
DCOM- 20210408
LR  - 20210408
IS  - 2040-3372 (Electronic)
IS  - 2040-3364 (Linking)
VI  - 12
IP  - 20
DP  - 2020 May 28
TI  - Pristine graphene induces innate immune training.
PG  - 11192-11200
LID - 10.1039/c9nr09661b [doi]
AB  - Graphene-based materials are of increasing interest for their potential use in 
      biomedical applications. However, there is a need to gain a deeper understanding 
      of how graphene modulates biological responses before moving towards clinical 
      application. Innate immune training is a recently described phenomenon whereby 
      cells of the innate immune system are capable of being programmed to generate an 
      increased non-specific response upon subsequent challenge. This has been well 
      established in the case of certain microbes and microbial products. However, 
      little is known about the capacity of particulate materials, such as pristine 
      graphene (pGr), to promote innate immune training. Here we report for the first 
      time that while stimulation with pGr alone does not directly induce cytokine 
      secretion by bone-marrow derived macrophages (BMDMs), it programs them for 
      enhanced secretion of proinflammatory cytokines (IL-6, TNF-alpha) and a concomitant 
      decrease in production of the regulatory cytokine, IL-10 after Toll-like receptor 
      (TLR) ligand stimulation. This capacity of pGr to program cells for enhanced 
      inflammatory responses could be overcome if the nanomaterial is incorporated in a 
      collagen matrix. Our findings thus demonstrate the potential of graphene to 
      modulate innate immunity over long timescales and have implications for the 
      design and biomedical use of pGr-based materials.
FAU - Lebre, Filipa
AU  - Lebre F
AD  - Adjuvant Research Group, School of Biochemistry and Immunology, Trinity 
      Biomedical Ssciences Institute, Trinity College Dublin, Dublin 2, D02 PN40, 
      Ireland. lavellee@tcd.ie.
FAU - Boland, John B
AU  - Boland JB
FAU - Gouveia, Pedro
AU  - Gouveia P
FAU - Gorman, Aoife L
AU  - Gorman AL
FAU - Lundahl, Mimmi L E
AU  - Lundahl MLE
FAU - I Lynch, Roisin
AU  - I Lynch R
FAU - O'Brien, Fergal J
AU  - O'Brien FJ
FAU - Coleman, Jonathan
AU  - Coleman J
FAU - Lavelle, Ed C
AU  - Lavelle EC
LA  - eng
PT  - Journal Article
DEP - 20200514
PL  - England
TA  - Nanoscale
JT  - Nanoscale
JID - 101525249
RN  - 0 (Fullerenes)
RN  - 0 (Monokines)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (pristine (C60))
SB  - IM
MH  - Animals
MH  - Fullerenes/chemistry/*pharmacology
MH  - Immunity, Innate/*drug effects
MH  - Macrophages/cytology/*immunology
MH  - Mice
MH  - Monokines/*immunology
MH  - Toll-Like Receptors/*immunology
EDAT- 2020/05/15 06:00
MHDA- 2021/04/10 06:00
CRDT- 2020/05/15 06:00
PHST- 2020/05/15 06:00 [pubmed]
PHST- 2021/04/10 06:00 [medline]
PHST- 2020/05/15 06:00 [entrez]
AID - 10.1039/c9nr09661b [doi]
PST - ppublish
SO  - Nanoscale. 2020 May 28;12(20):11192-11200. doi: 10.1039/c9nr09661b. Epub 2020 May 
      14.