PMID- 32407894
OWN - NLM
STAT- MEDLINE
DCOM- 20210726
LR  - 20210927
IS  - 1522-9653 (Electronic)
IS  - 1063-4584 (Print)
IS  - 1063-4584 (Linking)
VI  - 28
IP  - 8
DP  - 2020 Aug
TI  - Proteomic comparison of osteoarthritic and reference human menisci using 
      data-independent acquisition mass spectrometry.
PG  - 1092-1101
LID - S1063-4584(20)30994-8 [pii]
LID - 10.1016/j.joca.2020.05.001 [doi]
AB  - OBJECTIVE: Recent research in knee osteoarthritis (OA) highlights the role of the 
      meniscus in OA pathology. Our aim was to compare the proteomes of medial and 
      lateral menisci from end-stage medial compartment knee OA patients, with 
      reference menisci from knee-healthy deceased donors, using mass spectrometry. 
      DESIGN: Tissue plugs of O3 mm were obtained from the posterior horns of the 
      lateral and medial menisci from one knee of 10 knee-healthy deceased donors and 
      10 patients undergoing knee replacement. Proteins were extracted and prepared for 
      mass spectrometric analysis. Statistical analysis was conducted on abundance data 
      that was log(2)-transformed, using a linear mixed effects model and evaluated 
      using pathway analysis. RESULTS: We identified a total of 835 proteins in all 
      samples, of which 331 were included in the statistical analysis. The largest 
      differences could be seen between the medial menisci from OA patients and 
      references, with most proteins showing higher intensities in the medial menisci 
      from OA patients. Several matrix proteins, e.g., matrix metalloproteinase 3 
      (MMP3) (4.3 times higher values [95%CI 1.8, 10.6]), TIMP1 (3.5 [1.4, 8.5]), 
      asporin (4.1 [1.7, 10.0]) and versican (4.4 [1.8, 10.9]), all showed higher 
      abundance in medial menisci from OA patients compared to medial reference 
      menisci. OA medial menisci also showed increased activation of several pathways 
      involved in inflammation. CONCLUSION: An increase in protein abundance for 
      proteins such as MMP and TIMP1 in the medial menisci from OA patients suggests 
      simultaneous activation of both catabolic and anabolic processes that warrants 
      further attention.
CI  - Copyright (c) 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.
FAU - Folkesson, E
AU  - Folkesson E
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden; Lund University, Faculty 
      of Medicine, Department of Clinical Sciences Lund, Rheumatology and Molecular 
      Skeletal Biology, Lund, Sweden. Electronic address: elin.folkesson@med.lu.se.
FAU - Turkiewicz, A
AU  - Turkiewicz A
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden.
FAU - Ali, N
AU  - Ali N
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden.
FAU - Ryden, M
AU  - Ryden M
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden.
FAU - Hughes, H V
AU  - Hughes HV
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden.
FAU - Tjornstrand, J
AU  - Tjornstrand J
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden; Department of 
      Orthopaedics, Skane University Hospital, Lund, Sweden.
FAU - Onnerfjord, P
AU  - Onnerfjord P
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Rheumatology and Molecular Skeletal Biology, Lund, Sweden.
FAU - Englund, M
AU  - Englund M
AD  - Lund University, Faculty of Medicine, Department of Clinical Sciences Lund, 
      Orthopaedics, Clinical Epidemiology Unit, Lund, Sweden; Clinical Epidemiology 
      Research and Training Unit, Boston University School of Medicine, Boston, MA, 
      USA.
LA  - eng
GR  - 771121/ERC_/European Research Council/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200511
PL  - England
TA  - Osteoarthritis Cartilage
JT  - Osteoarthritis and cartilage
JID - 9305697
RN  - 0 (ASPN protein, human)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (VCAN protein, human)
RN  - 126968-45-4 (Versicans)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Case-Control Studies
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Female
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Male
MH  - Mass Spectrometry
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Menisci, Tibial/*metabolism
MH  - Middle Aged
MH  - Osteoarthritis, Knee/*metabolism
MH  - *Proteomics
MH  - Tissue Inhibitor of Metalloproteinase-1/metabolism
MH  - Versicans/metabolism
PMC - PMC7397514
OTO - NOTNLM
OT  - Extracellular matrix
OT  - Mass spectrometry
OT  - Meniscus
OT  - Osteoarthritis
EDAT- 2020/05/15 06:00
MHDA- 2021/07/27 06:00
PMCR- 2020/08/01
CRDT- 2020/05/15 06:00
PHST- 2020/01/30 00:00 [received]
PHST- 2020/04/09 00:00 [revised]
PHST- 2020/05/01 00:00 [accepted]
PHST- 2020/05/15 06:00 [pubmed]
PHST- 2021/07/27 06:00 [medline]
PHST- 2020/05/15 06:00 [entrez]
PHST- 2020/08/01 00:00 [pmc-release]
AID - S1063-4584(20)30994-8 [pii]
AID - 10.1016/j.joca.2020.05.001 [doi]
PST - ppublish
SO  - Osteoarthritis Cartilage. 2020 Aug;28(8):1092-1101. doi: 
      10.1016/j.joca.2020.05.001. Epub 2020 May 11.