PMID- 32409724
OWN - NLM
STAT- MEDLINE
DCOM- 20201130
LR  - 20210514
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 10
IP  - 1
DP  - 2020 May 14
TI  - Prostaglandin F2alpha agonist-induced suppression of 3T3-L1 cell adipogenesis affects 
      spatial formation of extra-cellular matrix.
PG  - 7958
LID - 10.1038/s41598-020-64674-1 [doi]
LID - 7958
AB  - To establish a deepening of the upper eyelid sulcus (DUES) model that can be 
      induced by prostaglandin (PG) analogues, a three-dimension (3D) tissue culture 
      was employed. Upon adipogenesis of the 3T3-L1 organoid, the effects of either 
      Bimatoprost acid (BIM-A), or PGF2alpha were examined. During the adipogenesis, 
      organoid size, lipid staining by BODIPY and expression of the extracellular 
      matrix (ECM) by immunocytochemistry and/or quantitative PCR were employed. The 
      size of the organoid increased remarkably during the adipogenesis, while such 
      increases were significantly inhibited by the presence of PGF2alpha or BIM-A. BODIPY 
      positive lipid-laden cells significantly increased during the adipogenesis, while 
      in contrast they were greatly suppressed by the presence of PGF2alpha. Characteristic 
      and spatial changes in ECM expressions observed upon adipogenesis were greatly 
      modified by the presence of PGs. Our present study using a 3D tissue culture may 
      be a suitable strategy toward understanding disease etiology of DUES.
FAU - Ida, Yosuke
AU  - Ida Y
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Hikage, Fumihito
AU  - Hikage F
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan. hikage@sapmed.ac.jp.
FAU - Itoh, Kaku
AU  - Itoh K
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Ida, Haruka
AU  - Ida H
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
FAU - Ohguro, Hiroshi
AU  - Ohguro H
AD  - Departments of Ophthalmology, Sapporo Medical University School of Medicine, 
      Sapporo, Japan.
LA  - eng
PT  - Journal Article
DEP - 20200514
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - B7IN85G1HY (Dinoprost)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - Dinoprost/*agonists
MH  - Extracellular Matrix/*drug effects/*metabolism
MH  - Mice
MH  - Time Factors
PMC - PMC7224398
COIS- The authors declare no competing interests.
EDAT- 2020/05/16 06:00
MHDA- 2020/12/01 06:00
PMCR- 2020/05/14
CRDT- 2020/05/16 06:00
PHST- 2019/08/16 00:00 [received]
PHST- 2020/04/20 00:00 [accepted]
PHST- 2020/05/16 06:00 [entrez]
PHST- 2020/05/16 06:00 [pubmed]
PHST- 2020/12/01 06:00 [medline]
PHST- 2020/05/14 00:00 [pmc-release]
AID - 10.1038/s41598-020-64674-1 [pii]
AID - 64674 [pii]
AID - 10.1038/s41598-020-64674-1 [doi]
PST - epublish
SO  - Sci Rep. 2020 May 14;10(1):7958. doi: 10.1038/s41598-020-64674-1.