PMID- 32409945
OWN - NLM
STAT- MEDLINE
DCOM- 20210126
LR  - 20210622
IS  - 1432-0614 (Electronic)
IS  - 0175-7598 (Linking)
VI  - 104
IP  - 13
DP  - 2020 Jul
TI  - Lactobacillus plantarum FRT10 alleviated high-fat diet-induced obesity in mice 
      through regulating the PPARalpha signal pathway and gut microbiota.
PG  - 5959-5972
LID - 10.1007/s00253-020-10620-0 [doi]
AB  - Previous studies showed that probiotics supplementation contributed to alleviate 
      obesity. This work was to assess the efficacy of Lactobacillus plantarum FRT10 
      from sour dough in alleviating obesity in mice fed with a high-fat diet (HFD), 
      and the underlying mechanisms focusing on modulation of the gut microbiota 
      profile. Kunming mice were fed with a regular diet (CT), a high-fat diet (HFD), 
      and two HFDs containing low and high doses of L. plantarum FRT10 for 8 weeks. The 
      physiological and biochemical modulations in liver were analyzed. Cecal contents 
      were analyzed by high-throughput 16S ribosomal RNA sequencing. FRT10 
      supplementation significantly reduced body weight gain, fat weight, and liver 
      triacylglycerols (TGs) and alanine aminotransferase (ALT) concentrations 
      (P < 0.05). FRT10 significantly ameliorated the HFD-induced gut dysbiosis, as 
      evidenced by increased abundance of microbes, including Butyricicoccus, 
      Butyricimonas, Intestinimonas, Odoribacter, and Alistipes, and decreased 
      abundance of Desulfovibrionaceae, Roseburia, and Lachnoclostridium. 
      Lactobacillus, Bifidobacterium, and Akkermansia were markedly increased after 
      FRT10 intervention. In addition, real-time quantitative PCR revealed that FRT10 
      upregulated the mRNA expression levels of peroxisome proliferator-activated 
      receptor-alpha (PPARalpha) and carnitine palmitoyltransferase-1alpha (CPT1alpha), and 
      downregulated the mRNA expression levels of sterol regulatory element-binding 
      protein 1 (SREBP-1) and TG-synthesizing enzyme diacylglycerol acyltransferase 1 
      (DGAT1) in liver. These findings suggested that FRT10 had anti-obesity effects in 
      obese mice partly related to the activation of PPARalpha/CPT1alpha pathway. FRT10 can be 
      considered a single probiotic agent for preventing HFD-induced obesity in humans 
      and animals.
FAU - Cai, Hongying
AU  - Cai H
AD  - Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, 
      Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
AD  - National Engineering Research Center of Biological Feed, Beijing, 100081, China.
FAU - Wen, Zhiguo
AU  - Wen Z
AD  - Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, 
      Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Li, Xiumei
AU  - Li X
AD  - Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, 
      Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Meng, Kun
AU  - Meng K
AD  - Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, 
      Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China.
FAU - Yang, Peilong
AU  - Yang P
AD  - Key Laboratory of Feed Biotechnology, Ministry of Agriculture and Rural Affairs, 
      Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 
      100081, China. yangpeilong@caas.cn.
AD  - National Engineering Research Center of Biological Feed, Beijing, 100081, China. 
      yangpeilong@caas.cn.
LA  - eng
GR  - 2017YFD0400303/National Basic Research Program of China (973 Program) (CN)/
GR  - 31802081/National Natural Science Foundation of China/
GR  - ASTIP-FRI07/Agricultural Science and Technology Innovation Program of China/
PT  - Journal Article
DEP - 20200514
PL  - Germany
TA  - Appl Microbiol Biotechnol
JT  - Applied microbiology and biotechnology
JID - 8406612
RN  - 0 (PPAR alpha)
RN  - 0 (Ppara protein, mouse)
RN  - EC 2.3.1.21 (CPT1B protein, mouse)
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
SB  - IM
MH  - Animals
MH  - Carnitine O-Palmitoyltransferase/genetics/metabolism
MH  - Diet, High-Fat/adverse effects
MH  - Female
MH  - Gastrointestinal Microbiome/*drug effects
MH  - Lactobacillus plantarum/*physiology
MH  - Lipid Metabolism/drug effects/genetics
MH  - Liver/drug effects/metabolism
MH  - Mice
MH  - Mice, Obese
MH  - Obesity/*drug therapy/metabolism/microbiology
MH  - PPAR alpha/genetics/*metabolism
MH  - Probiotics/*administration & dosage/pharmacology
MH  - Signal Transduction/*drug effects
MH  - Weight Gain/drug effects
OTO - NOTNLM
OT  - Gut microbiota
OT  - Lactobacillus plantarum FRT10
OT  - Obesity
OT  - PPARalpha
OT  - Sour dough
EDAT- 2020/05/16 06:00
MHDA- 2021/01/27 06:00
CRDT- 2020/05/16 06:00
PHST- 2019/11/22 00:00 [received]
PHST- 2020/04/09 00:00 [accepted]
PHST- 2020/03/31 00:00 [revised]
PHST- 2020/05/16 06:00 [pubmed]
PHST- 2021/01/27 06:00 [medline]
PHST- 2020/05/16 06:00 [entrez]
AID - 10.1007/s00253-020-10620-0 [pii]
AID - 10.1007/s00253-020-10620-0 [doi]
PST - ppublish
SO  - Appl Microbiol Biotechnol. 2020 Jul;104(13):5959-5972. doi: 
      10.1007/s00253-020-10620-0. Epub 2020 May 14.