PMID- 32410829
OWN - NLM
STAT- MEDLINE
DCOM- 20210308
LR  - 20210308
IS  - 1449-1907 (Electronic)
IS  - 1449-1907 (Linking)
VI  - 17
IP  - 8
DP  - 2020
TI  - Tendril extract of Cucurbita moschata suppresses NLRP3 inflammasome activation in 
      murine macrophages and human trophoblast cells.
PG  - 1006-1014
LID - 10.7150/ijms.39003 [doi]
AB  - Inflammation is the root cause of many diseases that pose a serious threat to 
      human health. Excessive inflammation can also result in preterm birth or 
      miscarriage in pregnant women. Pumpkin (Cucurbita moschata Duchesne, CMD) is a 
      well-known traditional health food and medicinal herb used in many countries to 
      treat diabetes, obesity, osteoporosis, cancer and other diseases. In this study, 
      we investigated the effects of hot water extract derived from the tendrils of C. 
      moschata Duchesne (TCMD) on NLRP3 inflammasome activation in murine macrophages 
      and human trophoblast cells. The TCMD treatment of LPS-primed bone marrow-derived 
      macrophages (BMDMs) and human trophoblast cells attenuated NLRP3 inflammasome 
      activation induced by inflammasome activators such as ATP, nigericin, and 
      monosodium urate (MSU). TCMD treatment suppressed IL-1beta secretion in a 
      dose-dependent manner, without affecting IL-6 secretion. In addition, TCMD 
      inhibited NLRP3-dependent pyroptosis in BMDMs. TCMD also suppressed the release 
      of mature IL-1beta and activation of cleaved-caspase-1 via limited ASC 
      oligomerization. Furthermore, TCMD significantly inhibited IL-1beta secretion and 
      pyroptotic cell death in human trophoblast cells. These results suggest that TCMD 
      exhibits anti-inflammatory effects mediated via inhibition of NLRP3 inflammasome 
      activation suggesting therapeutic potential against inflammatory diseases, 
      preterm birth, and miscarriage.
CI  - (c) The author(s).
FAU - Park, Ji-Yeon
AU  - Park JY
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical 
      Institute, Chonnam National University, Gwangju, Republic of Korea.
FAU - Jo, Sung-Gang
AU  - Jo SG
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical 
      Institute, Chonnam National University, Gwangju, Republic of Korea.
FAU - Lee, Ha-Nul
AU  - Lee HN
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Choi, Joo-Hee
AU  - Choi JH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical 
      Institute, Chonnam National University, Gwangju, Republic of Korea.
AD  - Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, 
      Republic of Korea.
FAU - Lee, Yeon-Ji
AU  - Lee YJ
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical 
      Institute, Chonnam National University, Gwangju, Republic of Korea.
FAU - Kim, Young-Min
AU  - Kim YM
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Cho, Jeong-Yong
AU  - Cho JY
AD  - Department of Food Science & Technology, Chonnam National University, Gwangju, 
      Republic of Korea.
FAU - Lee, Sung Ki
AU  - Lee SK
AD  - Department of Obstetrics and Gynecology, College of Medicine, Konyang University, 
      Daejeon, Republic of Korea.
FAU - Park, Jong-Hwan
AU  - Park JH
AD  - Laboratory Animal Medicine, College of Veterinary Medicine and Animal Medical 
      Institute, Chonnam National University, Gwangju, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20200406
PL  - Australia
TA  - Int J Med Sci
JT  - International journal of medical sciences
JID - 101213954
RN  - 0 (Inflammasomes)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Plant Extracts)
SB  - IM
MH  - Abortion, Spontaneous/immunology/prevention & control
MH  - Animals
MH  - Cell Line
MH  - Cucurbita/*chemistry
MH  - Female
MH  - Humans
MH  - Inflammasomes/*drug effects/immunology
MH  - Inflammation/drug therapy/immunology
MH  - Lipopolysaccharides/immunology
MH  - Macrophages/*drug effects/immunology
MH  - Male
MH  - Mice
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/antagonists & inhibitors/metabolism
MH  - Plant Extracts/*pharmacology/therapeutic use
MH  - Pregnancy
MH  - Premature Birth/immunology/prevention & control
MH  - Primary Cell Culture
MH  - Pyroptosis/drug effects/immunology
MH  - Trophoblasts/*drug effects/immunology
PMC - PMC7211156
OTO - NOTNLM
OT  - NLRP3 inflammasome
OT  - SW.71
OT  - bone marrow derived macrophage
OT  - tendril of Cucurbita moschata Duch
OT  - trophoblast
COIS- Competing Interests: The authors have declared that no competing interest exists.
EDAT- 2020/05/16 06:00
MHDA- 2021/03/09 06:00
PMCR- 2020/01/01
CRDT- 2020/05/16 06:00
PHST- 2019/08/04 00:00 [received]
PHST- 2020/01/08 00:00 [accepted]
PHST- 2020/05/16 06:00 [entrez]
PHST- 2020/05/16 06:00 [pubmed]
PHST- 2021/03/09 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - ijmsv17p1006 [pii]
AID - 10.7150/ijms.39003 [doi]
PST - epublish
SO  - Int J Med Sci. 2020 Apr 6;17(8):1006-1014. doi: 10.7150/ijms.39003. eCollection 
      2020.