PMID- 32410875
OWN - NLM
STAT- MEDLINE
DCOM- 20210709
LR  - 20210730
IS  - 1466-1861 (Electronic)
IS  - 0962-9351 (Print)
IS  - 0962-9351 (Linking)
VI  - 2020
DP  - 2020
TI  - lncRNA IGHCgamma1 Acts as a ceRNA to Regulate Macrophage Inflammation via the 
      miR-6891-3p/TLR4 Axis in Osteoarthritis.
PG  - 9743037
LID - 10.1155/2020/9743037 [doi]
LID - 9743037
AB  - Accumulating data have implicated that long noncoding RNA (lncRNA) plays an 
      important role in osteoarthritis (OA), which may function as a competitive 
      endogenous RNA (ceRNA) of microRNAs (miRNAs). lncRNA IGHCgamma1 has been demonstrated 
      to regulate inflammation and autoimmunity. Nonetheless, the altering effect of 
      IGHCgamma1 in OA remains unclear. This study is aimed at investigating the mechanism 
      and function of lncRNA IGHCgamma1 in OA. CCK-8, EdU, and transwell assays were used 
      to estimate macrophage proliferation and migration. Fluorescence in situ 
      hybridization (FISH) was performed to estimate the local expression of lncRNA 
      IGHCgamma1 in macrophages. Luciferase reporter assay was adopted to validate the 
      ceRNA role of IGHCgamma1 as miRNA sponge. lncRNA IGHCgamma1 was primarily localized in 
      macrophage cytoplasm and upregulated in OA. miR-6891-3p inhibited macrophage 
      proliferation, migration, and inflammatory response by targeting TLR4, while 
      lncRNA IGHCgamma1 promoted TLR4 expression by functioning as a ceRNA for miR-6891-3p 
      through the NF-kappaB signal in macrophages. This study strongly supports that lncRNA 
      IGHCgamma1 regulates inflammatory response via regulating the miR-6891-3p/TLR4/NF-kappaB 
      axis in macrophages.
CI  - Copyright (c) 2020 Pengjun Zhang et al.
FAU - Zhang, Pengjun
AU  - Zhang P
AD  - Department of Joint Surgery, The Affiliated Hospital of Qingdao University, 
      Qingdao University, Qingdao 266100, China.
AD  - Department of Emergency Surgery, People's Hospital of Rizhao, Rizhao 276800, 
      China.
FAU - Sun, Jianmei
AU  - Sun J
AD  - Department of Chemistry, School of Applied Chemistry, Food and Drug, Weifang 
      Engineering Vocational College, Qingzhou 262500, China.
FAU - Liang, Caihong
AU  - Liang C
AD  - Department of Cardiovasology, The Affiliated Jiangning Hospital of Nanjing 
      Medical University, Nanjing 210000, China.
FAU - Gu, Bingjie
AU  - Gu B
AD  - Department of Rheumatology and Immunology, Nanjing First Hospital, Nanjing 
      Medical University, Nanjing 210006, China.
FAU - Xu, Yang
AU  - Xu Y
AD  - National Center for Occupational Safety and Health, NHC, Beijing 100023, China.
FAU - Lu, Hongying
AU  - Lu H
AD  - Functional Laboratory, Weifang Medical University, Weifang 261000, China.
FAU - Cao, Bo
AU  - Cao B
AUID- ORCID: 0000-0003-4621-9924
AD  - Department of Emergency Medicine, Affiliated Hospital of Weifang Medical 
      University, Weifang 261000, China.
FAU - Xu, Hao
AU  - Xu H
AUID- ORCID: 0000-0003-2809-5243
AD  - Department of Joint Surgery, The Affiliated Hospital of Qingdao University, 
      Qingdao University, Qingdao 266100, China.
LA  - eng
PT  - Journal Article
DEP - 20200117
PL  - United States
TA  - Mediators Inflamm
JT  - Mediators of inflammation
JID - 9209001
RN  - 0 (MIRN6891 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Apoptosis/drug effects
MH  - Autoimmunity
MH  - Case-Control Studies
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Inflammation
MH  - Macrophages/*metabolism
MH  - Male
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - NF-kappa B/metabolism
MH  - Osteoarthritis/*metabolism
MH  - RNA, Long Noncoding/*metabolism
MH  - Signal Transduction/genetics
MH  - Toll-Like Receptor 4/*metabolism
PMC - PMC7201504
COIS- All authors have no conflicts of interest to disclose.
EDAT- 2020/05/16 06:00
MHDA- 2021/07/10 06:00
PMCR- 2020/01/17
CRDT- 2020/05/16 06:00
PHST- 2019/11/08 00:00 [received]
PHST- 2020/01/04 00:00 [accepted]
PHST- 2020/05/16 06:00 [entrez]
PHST- 2020/05/16 06:00 [pubmed]
PHST- 2021/07/10 06:00 [medline]
PHST- 2020/01/17 00:00 [pmc-release]
AID - 10.1155/2020/9743037 [doi]
PST - epublish
SO  - Mediators Inflamm. 2020 Jan 17;2020:9743037. doi: 10.1155/2020/9743037. 
      eCollection 2020.