PMID- 32411183
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1664-8021 (Print)
IS  - 1664-8021 (Electronic)
IS  - 1664-8021 (Linking)
VI  - 11
DP  - 2020
TI  - The Prognostic Signature and Potential Target Genes of Six Long Non-coding RNA in 
      Laryngeal Squamous Cell Carcinoma.
PG  - 413
LID - 10.3389/fgene.2020.00413 [doi]
LID - 413
AB  - Studies have shown that long non-coding RNA (lncRNA) may act as the carcinogenic 
      factor or tumor suppressor of laryngeal squamous cell carcinoma (LSCC). This 
      study aims to identify the prognostic value and potential target protein-coding 
      genes (PCGs) of lncRNAs in LSCC. The LSCC datasets were collected from The Cancer 
      Genome Atlas (TCGA). Statistical and bioinformatic methods were used to establish 
      and evaluate the prognostic model, identify the correlation between lncRNAs and 
      clinical characteristics, and screen for PCGs co-expressed with lncRNAs. Weighted 
      gene co-expression network analysis (WGCNA) identified PCG modules associated 
      with clinical characteristics. The expression of lncRNAs and PCGs was analyzed 
      using our LSCC patients by RT-qPCR. LINC02154, LINC00528, SPRY4-AS1, TTTY14, 
      LNCSRLR, and KLHL7-DT were selected to establish the prognostic model. The 
      overall survival (OS) of low-risk patients forecasted by the model was 
      significantly better than high-risk patients. Receiver operating characteristic 
      (ROC) curve and concordance index (C-index) validated the accuracy of the 
      prognostic model. Chi-square test showed that six lncRNAs were associated with 
      one of the clinical characteristics, i.e., gender, clinical stage, T and N stage, 
      respectively. WGCNA identified PCG modules associated with gender, clinical 
      stage, T and N stage. We took the intersection of the PCG modules of WGCNA, the 
      differentially expressed PCGs between LSCC and normal samples, and the PCGs 
      co-expressed with six lncRNAs. The intersection PCGs survival analysis showed 
      that four PCGs, i.e., STC2, TSPAN9, SMS, and TCEA3 affected the OS of LSCC. More 
      importantly, the differential expression of six lncRNAs and four PCGs between 
      LSCC and normal samples was verified by our LSCC patients. In conclusion, we 
      successfully established a prognostic model based on six-lncRNA RiskScore and 
      initially screened the potential target PCGs of six lncRNAs for further basic and 
      clinical research.
CI  - Copyright (c) 2020 Gong, Xu, Zhang, Shan and Zhang.
FAU - Gong, Shiqi
AU  - Gong S
AD  - Department of Otolaryngology-Head and Neck Surgery, Ruijin Hospital, School of 
      Medicine, Shanghai Jiao Tong University, Shanghai, China.
FAU - Xu, Meng
AU  - Xu M
AD  - Department of Radiation Oncology, The First Affiliated Hospital, Anhui Medical 
      University, Hefei, China.
FAU - Zhang, Yiyun
AU  - Zhang Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Ruijin Hospital, School of 
      Medicine, Shanghai Jiao Tong University, Shanghai, China.
FAU - Shan, Yamin
AU  - Shan Y
AD  - Department of Otolaryngology-Head and Neck Surgery, Ruijin Hospital, School of 
      Medicine, Shanghai Jiao Tong University, Shanghai, China.
FAU - Zhang, Hao
AU  - Zhang H
AD  - Department of Otolaryngology-Head and Neck Surgery, Ruijin Hospital, School of 
      Medicine, Shanghai Jiao Tong University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20200428
PL  - Switzerland
TA  - Front Genet
JT  - Frontiers in genetics
JID - 101560621
PMC - PMC7198905
OTO - NOTNLM
OT  - WGCNA
OT  - bioinformatic analysis
OT  - laryngeal squamous cell carcinoma
OT  - long non-coding RNA
OT  - prognostic signature
EDAT- 2020/05/16 06:00
MHDA- 2020/05/16 06:01
PMCR- 2020/04/28
CRDT- 2020/05/16 06:00
PHST- 2020/01/29 00:00 [received]
PHST- 2020/04/01 00:00 [accepted]
PHST- 2020/05/16 06:00 [entrez]
PHST- 2020/05/16 06:00 [pubmed]
PHST- 2020/05/16 06:01 [medline]
PHST- 2020/04/28 00:00 [pmc-release]
AID - 10.3389/fgene.2020.00413 [doi]
PST - epublish
SO  - Front Genet. 2020 Apr 28;11:413. doi: 10.3389/fgene.2020.00413. eCollection 2020.