PMID- 32413475
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240227
IS  - 1879-3169 (Electronic)
IS  - 0378-4274 (Linking)
VI  - 330
DP  - 2020 May 13
TI  - Human monocyte-derived dendritic cells as an in vitro alternative model cell to 
      evaluate the immunotoxicity of 2, 4-Dinitrochlorobenzene.
PG  - 118-127
LID - S0378-4274(20)30141-7 [pii]
LID - 10.1016/j.toxlet.2020.05.008 [doi]
AB  - Improvements in science and technology have led to the increasing threats of new 
      chemicals to the public health. It is crucial to evaluate the toxicity, 
      especially immunotoxicology. Dendritic cells (DCs) are believed to be more 
      favorable choices in immunotoxicity evaluations. To obtain and evaluate the value 
      of human monocyte-derived immature DCs (imDCs) in vitro applications in 
      immunotoxicology, compared the results in vitro. DCs were obtained from enriched 
      leukocytes of peripheral blood by using magnetic cell sorting and cytokine 
      (rhGM-CSF + rhIL-4) co-induction. imDCs function in vitro and the surface 
      antigens changes both in imDCs and THP-1 after 24 h of 2,4-dinitrochlorobenzene 
      (DNCB) exposure were determined. The results were compared with those of 
      DNCB-induced rats. The feasibility of imDCs applications in immunotoxicology was 
      evaluated. In vivo, the splenic nodules, lymphocytes, and CD103(+)DC surface 
      antigen expression were altered in the spleen of DNCB-induced rats. Moreover, 
      DNCB exposure increased CD8(+) T cell numbers both in peripheral blood and in the 
      spleen of DNCB-induced rats. In vitro, DNCB exposure reduced the antigen uptake 
      capacity and enhanced the T cell proliferative capacity of imDCs. The results are 
      consistent with in vivo, but superior to that of the THP-1. Our results suggest 
      that human monocyte-derived DCs may have potential applications as an attractive 
      in vitro alternative cell model to evaluate the sensitization of DNCB.
CI  - Copyright (c) 2020. Published by Elsevier B.V.
FAU - Yao, Wu
AU  - Yao W
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Ding, Mingcui
AU  - Ding M
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Bao, Lei
AU  - Bao L
AD  - School of Public Health, Hebei Medical University, Shijiazhuang, Hebei, 050000, 
      China.
FAU - Zhao, Youliang
AU  - Zhao Y
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Wang, Di
AU  - Wang D
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Li, Yiping
AU  - Li Y
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Qu, YaQian
AU  - Qu Y
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China.
FAU - Hao, Changfu
AU  - Hao C
AD  - Department of Occupational Health and Occupational Disease, School of Public 
      Health, Zhengzhou University, Zhengzhou, 450001, Henan, China. Electronic 
      address: haochangfu@126.com.
LA  - eng
PT  - Journal Article
DEP - 20200513
PL  - Netherlands
TA  - Toxicol Lett
JT  - Toxicology letters
JID - 7709027
SB  - IM
OTO - NOTNLM
OT  - DNCB
OT  - Dendritic cells
OT  - Immunotoxicity
OT  - Sensitization
OT  - THP-1
COIS- Declaration of Competing Interest The authors declare that they have no conflict 
      of interest.
EDAT- 2020/05/16 06:00
MHDA- 2020/05/16 06:01
CRDT- 2020/05/16 06:00
PHST- 2020/01/09 00:00 [received]
PHST- 2020/04/11 00:00 [revised]
PHST- 2020/05/07 00:00 [accepted]
PHST- 2020/05/16 06:01 [medline]
PHST- 2020/05/16 06:00 [pubmed]
PHST- 2020/05/16 06:00 [entrez]
AID - S0378-4274(20)30141-7 [pii]
AID - 10.1016/j.toxlet.2020.05.008 [doi]
PST - aheadofprint
SO  - Toxicol Lett. 2020 May 13;330:118-127. doi: 10.1016/j.toxlet.2020.05.008.