PMID- 32415600
OWN - NLM
STAT- MEDLINE
DCOM- 20210315
LR  - 20210315
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 2146
DP  - 2020
TI  - Analysis of Arbuscular Mycorrhizal Fungal Communities by Terminal Restriction 
      Fragment Length Polymorphism (TRFLP).
PG  - 117-136
LID - 10.1007/978-1-0716-0603-2_10 [doi]
AB  - Terminal restriction fragment length polymorphism (TRFLP) approaches enable the 
      detection and identification of microbial taxa into samples coming from root or 
      soil material DNA extraction. The low taxonomic diversity of arbuscular 
      mycorrhizal (AM) fungi makes this technique a cheap and adequate method for 
      fingerprinting their communities. Here, I describe the TRFLP database approach, a 
      version of the technique in which the AM fungal taxa present in the sample pool 
      is identified for, later, match their presence in the different samples contained 
      in the experiment. A final AM fungal operational taxonomic unit x sample 
      presence-absence matrix is obtained, which allows the subsequent multivariate 
      statistical analysis of the experimental results.
FAU - Lopez-Garcia, Alvaro
AU  - Lopez-Garcia A
AD  - Department of Soil Microbiology and Symbiotic Systems, Estacion Experimental del 
      Zaidin, CSIC, Granada, Spain. alvaro.lopez@eez.csic.es.
AD  - Department of Animal Biology, Plant Biology and Ecology, Universidad de Jaen, 
      Jaen, Spain. alvaro.lopez@eez.csic.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA, Fungal)
SB  - IM
MH  - Chromosome Mapping
MH  - DNA, Fungal/genetics/*isolation & purification
MH  - Genotyping Techniques/*methods
MH  - Mycobiome/genetics
MH  - Mycorrhizae/genetics/*isolation & purification
MH  - Plant Roots/genetics
MH  - Polymorphism, Restriction Fragment Length/*genetics
MH  - Soil Microbiology
OTO - NOTNLM
OT  - Community assembly
OT  - Fingerprinting
OT  - Operational taxonomic units
OT  - Phylogenetic analyses
OT  - R software
OT  - TRAMPR package
EDAT- 2020/05/18 06:00
MHDA- 2021/03/16 06:00
CRDT- 2020/05/17 06:00
PHST- 2020/05/17 06:00 [entrez]
PHST- 2020/05/18 06:00 [pubmed]
PHST- 2021/03/16 06:00 [medline]
AID - 10.1007/978-1-0716-0603-2_10 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2020;2146:117-136. doi: 10.1007/978-1-0716-0603-2_10.