PMID- 32422267
OWN - NLM
STAT- MEDLINE
DCOM- 20210312
LR  - 20210312
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 160
DP  - 2020 Oct 1
TI  - Immobilized polyphenol oxidase: Preparation, optimization and oxidation of 
      phenolic compounds.
PG  - 233-244
LID - S0141-8130(20)33234-7 [pii]
LID - 10.1016/j.ijbiomac.2020.05.079 [doi]
AB  - Polyphenol oxidase (PPO) was immobilized on chitosan/montmorillonite (CTS/MMT) 
      and chitosan-gold nanoparticles/montmorillonite (CTS-AuNPs/MMT) composites, 
      respectively. Taguchi method was applied to determine the optimal immobilization 
      conditions for achieving the maximum enzyme activity. PPO immobilized on CTS/MMT 
      (IPPO) and CTS-AuNPs/MMT (IPPO-Au) showed the highest enzyme activity at 
      15.61 x 10(3) and 29.01 x 10(3) U/g, respectively. IPPO-Au exhibited the higher 
      stability and reusability than that of IPPO. The bio-catalytic performance of 
      immobilized PPO was evaluated for the removal of phenol (PH), 4-chlorophenol 
      (4-CP) and 2,4-dichlorophenol (2,4-DCP) in aqueous solution. The effects of pH, 
      temperature, enzyme/substrate ratio, substrate concentration, reaction time on 
      the phenolic compounds removal were investigated in detail. The results showed 
      that, for both immobilized PPO, the optimal pH was 7, 6 and 5 for PH, 4-CP and 
      2,4-DCP, respectively, and the optimal temperature was 30  degrees C for all substrate. 
      The optimal enzyme/substrate ratio and reaction time for IPPO-Au was lower than 
      that of IPPO proved the higher catalytic efficiency. Chlorophenols showed the 
      improved catalytic efficiency in comparison with PH for both immobilized PPO, 
      while the effect of substrate chemical structure on the IPPO-Au properties was 
      limited. The results suggested AuNPs presented in support played an important 
      role on the enzyme activity and stability of immobilized PPO.
CI  - Copyright (c) 2020 Elsevier B.V. All rights reserved.
FAU - Wang, Han
AU  - Wang H
AD  - Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean 
      University of China, Qingdao 266100, China.
FAU - Li, Shiqian
AU  - Li S
AD  - College of Ocean and Bio-chemical Engineering, Fujian Provincial Key Lab of 
      Coastal Basin Environment, Fuqing Branch of Fujian Normal University, Fuqing 
      350300, China.
FAU - Li, Jin
AU  - Li J
AD  - Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean 
      University of China, Qingdao 266100, China. Electronic address: lijin@ouc.edu.cn.
FAU - Zhong, Lian
AU  - Zhong L
AD  - College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 
      266100, China.
FAU - Cheng, Huali
AU  - Cheng H
AD  - Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean 
      University of China, Qingdao 266100, China.
FAU - Ma, Qimin
AU  - Ma Q
AD  - Key Laboratory of Marine Environment and Ecology, Ministry of Education, Ocean 
      University of China, Qingdao 266100, China.
LA  - eng
PT  - Journal Article
DEP - 20200516
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Chlorophenols)
RN  - 0 (Enzymes, Immobilized)
RN  - 0 (Phenols)
RN  - 1302-78-9 (Bentonite)
RN  - 3DLC36A01X (4-chlorophenol)
RN  - 7440-57-5 (Gold)
RN  - 9012-76-4 (Chitosan)
RN  - EC 1.10.3.1 (Catechol Oxidase)
SB  - IM
MH  - Bentonite/chemistry
MH  - Catalysis
MH  - Catechol Oxidase/*chemistry
MH  - Chitosan/chemistry
MH  - Chlorophenols/chemistry
MH  - Enzymes, Immobilized/*chemistry
MH  - Gold/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Kinetics
MH  - Metal Nanoparticles/chemistry
MH  - Oxidation-Reduction
MH  - Phenols/*chemistry
MH  - Temperature
OTO - NOTNLM
OT  - Chitosan/montmorillonite composites
OT  - Gold nanoparticles
OT  - Immobilization
OT  - Optimization
OT  - Phenolic compounds
OT  - Polyphenol oxidase
EDAT- 2020/05/19 06:00
MHDA- 2021/03/13 06:00
CRDT- 2020/05/19 06:00
PHST- 2019/12/30 00:00 [received]
PHST- 2020/05/12 00:00 [revised]
PHST- 2020/05/12 00:00 [accepted]
PHST- 2020/05/19 06:00 [pubmed]
PHST- 2021/03/13 06:00 [medline]
PHST- 2020/05/19 06:00 [entrez]
AID - S0141-8130(20)33234-7 [pii]
AID - 10.1016/j.ijbiomac.2020.05.079 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2020 Oct 1;160:233-244. doi: 10.1016/j.ijbiomac.2020.05.079. 
      Epub 2020 May 16.