PMID- 32422377 OWN - NLM STAT- MEDLINE DCOM- 20200930 LR - 20200930 IS - 1878-3511 (Electronic) IS - 1201-9712 (Linking) VI - 96 DP - 2020 Jul TI - Natural killer cell receptor variants and chronic hepatitis B virus infection in the Vietnamese population. PG - 541-547 LID - S1201-9712(20)30340-4 [pii] LID - 10.1016/j.ijid.2020.05.033 [doi] AB - OBJECTIVES: Genes of host immunity play an important role in disease pathogenesis and are determinants of clinical courses of infections, including hepatitis B virus (HBV). Killer-cell immunoglobulin-like receptor (KIR), expressed on the surface of natural killer cells (NK), regulate NK cell cytotoxicity by interacting with human leukocyte antigen (HLA) class I molecules and are candidates for influencing the course of HBV. This study evaluated whether variations in KIR gene content and HLA-C ligands are associated with HBV and with the development of liver cirrhosis and hepatocellular carcinoma. METHODS: A Vietnamese study cohort (HBV n = 511; controls n = 140) was genotyped using multiplex sequence-specific polymerase chain reaction (PCR-SSP) followed by melting curve analysis. RESULTS: The presence of the functional allelic group of KIR2DS4 was associated with an increased risk of chronic HBV (OR = 1.86, p(corr) = 0.02), while KIR2DL2+HLA-C1 (OR = 0.62, p(corr) = 0.04) and KIR2DL3+HLA-C1 (OR = 0.48, p(corr) = 0.04) were associated with a decreased risk. The pair KIR2DL3+HLA-C1 was associated with liver cirrhosis (OR = 0.40, p(corr) = 0.01). The presence of five or more activating KIR variants was associated with hepatocellular carcinoma (OR = 0.53, p(corr) = 0.04). CONCLUSIONS: KIR gene content variation and combinations KIR-HLA influence the outcome of HBV infection. CI - Copyright (c) 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved. FAU - Auer, Eduardo Delabio AU - Auer ED AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Tong, Hoang Van AU - Tong HV AD - Institute of Tropical Medicine, University of Tubingen, Tubingen, Germany; Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Viet Nam; Vietnamese German Center for Medical Research (VGCARE), Hanoi, Viet Nam. FAU - Amorim, Leonardo Maldaner AU - Amorim LM AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Malheiros, Danielle AU - Malheiros D AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Hoan, Nghiem Xuan AU - Hoan NX AD - Institute of Tropical Medicine, University of Tubingen, Tubingen, Germany; Vietnamese German Center for Medical Research (VGCARE), Hanoi, Viet Nam; Institute of Clinical Infectious Diseases, Hanoi, Viet Nam. FAU - Issler, Hellen Caroline AU - Issler HC AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Petzl-Erler, Maria Luiza AU - Petzl-Erler ML AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Beltrame, Marcia Holsbach AU - Beltrame MH AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Boldt, Angelica Beate Winter AU - Boldt ABW AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. FAU - Toan, Nguyen Linh AU - Toan NL AD - Department of Pathophysiology, Vietnam Military Medical University, Hanoi, Viet Nam; Vietnamese German Center for Medical Research (VGCARE), Hanoi, Viet Nam. FAU - Song, Le Huu AU - Song LH AD - Vietnamese German Center for Medical Research (VGCARE), Hanoi, Viet Nam; Institute of Clinical Infectious Diseases, Hanoi, Viet Nam. FAU - Velavan, Thirumalaisamy P AU - Velavan TP AD - Institute of Tropical Medicine, University of Tubingen, Tubingen, Germany; Vietnamese German Center for Medical Research (VGCARE), Hanoi, Viet Nam; Faculty of Medicine, Duy Tan University, Da Nang, Viet Nam. Electronic address: velavan@medizin.uni-tuebingen.de. FAU - Augusto, Danillo G AU - Augusto DG AD - Programa de Pos-Graduacao em Genetica, Departamento de Genetica, Universidade Federal do Parana (UFPR), Curitiba, Brazil. Electronic address: danillo@augusto.bio.br. LA - eng PT - Journal Article DEP - 20200516 PL - Canada TA - Int J Infect Dis JT - International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases JID - 9610933 RN - 0 (HLA-C Antigens) RN - 0 (KIR2DL2 protein, human) RN - 0 (KIR2DL3 protein, human) RN - 0 (KIR2DS4 protein, human) RN - 0 (Receptors, KIR) RN - 0 (Receptors, KIR2DL2) RN - 0 (Receptors, KIR2DL3) SB - IM MH - Adult MH - Aged MH - Alleles MH - Carcinoma, Hepatocellular/genetics/immunology/virology MH - Cohort Studies MH - Female MH - Genetic Variation MH - Genotype MH - HLA-C Antigens/genetics/immunology MH - Hepatitis B virus/genetics/immunology/*physiology MH - Hepatitis B, Chronic/*genetics/immunology/virology MH - Humans MH - Liver Cirrhosis/genetics/immunology/virology MH - Male MH - Middle Aged MH - Receptors, KIR/*genetics/immunology MH - Receptors, KIR2DL2/*genetics/immunology MH - Receptors, KIR2DL3/*genetics/immunology MH - Vietnam MH - Young Adult OTO - NOTNLM OT - Hepatitis B virus OT - Hepatocellular carcinoma OT - Human leukocyte antigen OT - Killer-cell immunoglobulin-like receptor OT - Liver cirrhosis OT - Natural killer cells EDAT- 2020/05/19 06:00 MHDA- 2020/10/02 06:00 CRDT- 2020/05/19 06:00 PHST- 2020/03/26 00:00 [received] PHST- 2020/05/05 00:00 [revised] PHST- 2020/05/07 00:00 [accepted] PHST- 2020/05/19 06:00 [pubmed] PHST- 2020/10/02 06:00 [medline] PHST- 2020/05/19 06:00 [entrez] AID - S1201-9712(20)30340-4 [pii] AID - 10.1016/j.ijid.2020.05.033 [doi] PST - ppublish SO - Int J Infect Dis. 2020 Jul;96:541-547. doi: 10.1016/j.ijid.2020.05.033. Epub 2020 May 16.