PMID- 32422642
OWN - NLM
STAT- MEDLINE
DCOM- 20211027
LR  - 20211027
IS  - 1423-0194 (Electronic)
IS  - 0028-3835 (Linking)
VI  - 111
IP  - 3
DP  - 2021
TI  - Lipid Droplets Accumulate in the Hypothalamus of Mice and Humans with and without 
      Metabolic Diseases.
PG  - 263-272
LID - 10.1159/000508735 [doi]
AB  - BACKGROUND: In peripheral tissues, the lipid droplet (LD) organelle links lipid 
      metabolism, inflammation, and insulin resistance. Little is known about the brain 
      LDs. OBJECTIVES: We hypothesized that hypothalamic LDs would be altered in 
      metabolic diseases. METHODS: We used immunofluorescence labeling of the specific 
      LD protein, PLIN2, as the approach to visualize and quantify LDs. RESULTS: LDs 
      were abundant in the hypothalamic third ventricle wall layer with similar 
      heterogeneous distributions between control mice and humans. The LD content was 
      enhanced by high-fat diet (HFD) in both wild-type and in low-density lipoprotein 
      receptor deficient (Ldlr -/- HFD) mice. Strikingly, we observed a lower LD amount 
      in type 2 diabetes mellitus (T2DM) patients when compared with non-T2DM patients. 
      CONCLUSIONS: LDs accumulate in the normal hypothalamus, with similar 
      distributions in human and mouse. Moreover, metabolic diseases differently modify 
      LD content in mouse and human. Our results suggest that hypothalamic LD 
      accumulation is an important target to the study of metabolism.
CI  - (c) 2020 The Author(s) Published by S. Karger AG, Basel.
FAU - Maya-Monteiro, Clarissa Menezes
AU  - Maya-Monteiro CM
AD  - Laboratory of Immunopharmacology, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz 
      (FIOCRUZ), Rio de Janeiro, Brazil, clarissamayam@gmail.com.
AD  - Laboratory of Endocrinology and Department of Endocrinology and Metabolism, 
      Amsterdam Neuroscience, Amsterdam University Medical Centers (UMC), University of 
      Amsterdam, Amsterdam, The Netherlands, clarissamayam@gmail.com.
AD  - Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands 
      Academy of Arts and Sciences, Amsterdam, The Netherlands, 
      clarissamayam@gmail.com.
FAU - Correa-da-Silva, Felipe
AU  - Correa-da-Silva F
AD  - Laboratory of Endocrinology and Department of Endocrinology and Metabolism, 
      Amsterdam Neuroscience, Amsterdam University Medical Centers (UMC), University of 
      Amsterdam, Amsterdam, The Netherlands.
FAU - Hofmann, Susanna S
AU  - Hofmann SS
AD  - Institute for Diabetes and Regeneration, Helmholtz Diabetes Center, Helmholtz 
      Zentrum Munchen, German Research Center for Environmental Health (GmbH), 
      Neuherberg, Germany.
FAU - Hesselink, Matthijs K C
AU  - Hesselink MKC
AD  - Department of Nutrition and Movement Sciences, Maastricht University Medical 
      Centre+ and NUTRIM School for Nutrition and Translational Research in Metabolism, 
      Maastricht, The Netherlands.
FAU - la Fleur, Susanne E
AU  - la Fleur SE
AD  - Laboratory of Endocrinology and Department of Endocrinology and Metabolism, 
      Amsterdam Neuroscience, Amsterdam University Medical Centers (UMC), University of 
      Amsterdam, Amsterdam, The Netherlands.
AD  - Netherlands Institute for Neuroscience, an Institute of the Royal Netherlands 
      Academy of Arts and Sciences, Amsterdam, The Netherlands.
FAU - Yi, Chun-Xia
AU  - Yi CX
AD  - Laboratory of Endocrinology and Department of Endocrinology and Metabolism, 
      Amsterdam Neuroscience, Amsterdam University Medical Centers (UMC), University of 
      Amsterdam, Amsterdam, The Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200518
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (PLIN2 protein, human)
RN  - 0 (Perilipin-2)
RN  - 0 (Receptors, LDL)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Animals
MH  - Autopsy
MH  - Diabetes Mellitus, Type 2/*metabolism
MH  - Diet, High-Fat
MH  - Disease Models, Animal
MH  - Female
MH  - Humans
MH  - Hypothalamus/*metabolism
MH  - Insulin Resistance/*physiology
MH  - Lipid Droplets/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Perilipin-2/*metabolism
MH  - Receptors, LDL/deficiency
MH  - Tissue Banks
OTO - NOTNLM
OT  - Hypothalamus
OT  - Insulin resistance
OT  - Lipid droplet
OT  - PLIN2
OT  - Type 2 diabetes mellitus
EDAT- 2020/05/19 06:00
MHDA- 2021/10/28 06:00
CRDT- 2020/05/19 06:00
PHST- 2019/11/14 00:00 [received]
PHST- 2020/05/15 00:00 [accepted]
PHST- 2020/05/19 06:00 [pubmed]
PHST- 2021/10/28 06:00 [medline]
PHST- 2020/05/19 06:00 [entrez]
AID - 000508735 [pii]
AID - 10.1159/000508735 [doi]
PST - ppublish
SO  - Neuroendocrinology. 2021;111(3):263-272. doi: 10.1159/000508735. Epub 2020 May 
      18.