PMID- 32424849 OWN - NLM STAT- MEDLINE DCOM- 20210730 LR - 20210730 IS - 1440-1746 (Electronic) IS - 0815-9319 (Linking) VI - 35 IP - 12 DP - 2020 Dec TI - Oligosaccharide-dependent anti-inflammatory role of galectin-1 for macrophages in ulcerative colitis. PG - 2158-2169 LID - 10.1111/jgh.15097 [doi] AB - BACKGROUND AND AIM: Galectin-1 plays a protective role against colitis by binding with polylactosamine structures on macrophages in beta-1,4-galactosyltransferase I-deficient mice, but the precise function of galectin-1 remains unknown. In the present study, we investigated the anti-inflammatory role of galectin-1 on macrophages to ameliorate ulcerative colitis in both animal model and human tissue samples. METHODS: The expression of galectin-1 in colonic tissues of ulcerative colitis patients was evaluated by immunohistochemistry. Cytokine production of mouse bone marrow-derived macrophages (BMDMs) cultured with galectin-1 was investigated. Galectin-1 binding capacity and polylactosamine expression in macrophages stimulated with lipopolysaccharides were evaluated by flow cytometry. BMDMs cultured with galectin-1 were transferred into Recombination activating gene (Rag) 2(-/-) mice, and the severity of the dextran sodium sulfate-induced colitis model was investigated. Furthermore, RNA sequencing was performed to characterize macrophages treated with galectin-1. RESULTS: In ulcerative colitis patients, tissue expression of galectin-1was decreased in inflamed mucosa compared with non-inflamed mucosa. Galectin-1 induced interleukin-10 production in BMDMs, and the interleukin-10 production was abrogated by lactose, which inhibits the interaction of oligosaccharide-galectin binding. Dextran sodium sulfate colitis was significantly ameliorated in Rag2(-/-) mice undergoing galectin-1-treated BMDM transfer compared with those undergoing vehicle-treated BMDM transfer. RNA sequencing revealed that treatment with galectin-1 increased the expression of CCAAT/enhancer binding protein beta and CD163, but decreased the expression of CD80 on BMDMs. CONCLUSION: Galectin-1, whose expression is decreased in the inflamed mucosa of ulcerative colitis patients, can ameliorate murine colitis by conferring oligosaccharide-dependent anti-inflammatory properties to macrophages. CI - (c) 2020 Journal of Gastroenterology and Hepatology Foundation and John Wiley & Sons Australia, Ltd. FAU - Iwatani, Shuko AU - Iwatani S AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Shinzaki, Shinichiro AU - Shinzaki S AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Amano, Takahiro AU - Amano T AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Otake, Yuriko AU - Otake Y AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Tani, Mizuki AU - Tani M AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Yoshihara, Takeo AU - Yoshihara T AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Tsujii, Yoshiki AU - Tsujii Y AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Hayashi, Yoshito AU - Hayashi Y AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Inoue, Takahiro AU - Inoue T AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Okuzaki, Daisuke AU - Okuzaki D AD - Genome Information Research Center, Research Institute for Microbial Diseases, Osaka University, Suita, Japan. FAU - Mizushima, Tsunekazu AU - Mizushima T AUID- ORCID: 0000-0002-0825-6823 AD - Department of Gastroenterological Surgery, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Miyoshi, Eiji AU - Miyoshi E AD - Department of Molecular Biochemistry and Clinical Investigation, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Iijima, Hideki AU - Iijima H AUID- ORCID: 0000-0002-5423-3059 AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. FAU - Takehara, Tetsuo AU - Takehara T AD - Department of Gastroenterology and Hepatology, Graduate School of Medicine, Osaka University, Suita, Japan. LA - eng GR - 16K09310/the Japan Society for the Promotion of Science/ GR - 16K09310/Japan Society for the Promotion of Science/ GR - Japan Society for the Promotion of Science/ PT - Journal Article DEP - 20200706 PL - Australia TA - J Gastroenterol Hepatol JT - Journal of gastroenterology and hepatology JID - 8607909 RN - 0 (Antigens, CD) RN - 0 (Antigens, Differentiation, Myelomonocytic) RN - 0 (B7-1 Antigen) RN - 0 (CCAAT-Enhancer-Binding Protein-beta) RN - 0 (CD163 antigen) RN - 0 (Galectin 1) RN - 0 (Oligosaccharides) RN - 0 (Receptors, Cell Surface) RN - 130068-27-8 (Interleukin-10) RN - J2B2A4N98G (Lactose) SB - IM MH - Animals MH - Antigens, CD/genetics/metabolism MH - Antigens, Differentiation, Myelomonocytic/genetics/metabolism MH - B7-1 Antigen/genetics/metabolism MH - CCAAT-Enhancer-Binding Protein-beta/genetics/metabolism MH - Colitis, Ulcerative/drug therapy/*genetics MH - Disease Models, Animal MH - Galectin 1/genetics/metabolism/*physiology/therapeutic use MH - *Gene Expression MH - Humans MH - Interleukin-10/metabolism MH - Intestinal Mucosa/metabolism MH - Lactose/pharmacology MH - Macrophages/*metabolism/*physiology MH - Mice, Inbred C57BL MH - *Oligosaccharides/metabolism MH - Protein Binding MH - Receptors, Cell Surface/genetics/metabolism OTO - NOTNLM OT - Cebpbeta OT - DSS-induced colitis OT - M2 macrophages OT - galectin-1 OT - polylactosamine EDAT- 2020/05/20 06:00 MHDA- 2021/07/31 06:00 CRDT- 2020/05/20 06:00 PHST- 2020/01/15 00:00 [received] PHST- 2020/05/05 00:00 [revised] PHST- 2020/05/10 00:00 [accepted] PHST- 2020/05/20 06:00 [pubmed] PHST- 2021/07/31 06:00 [medline] PHST- 2020/05/20 06:00 [entrez] AID - 10.1111/jgh.15097 [doi] PST - ppublish SO - J Gastroenterol Hepatol. 2020 Dec;35(12):2158-2169. doi: 10.1111/jgh.15097. Epub 2020 Jul 6.