PMID- 32425782
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200928
IS  - 1663-9812 (Print)
IS  - 1663-9812 (Electronic)
IS  - 1663-9812 (Linking)
VI  - 11
DP  - 2020
TI  - Si-Wei-Qing-Gan-Tang Improves Non-Alcoholic Steatohepatitis by Modulating the 
      Nuclear Factor-kappaB Signal Pathway and Autophagy in Methionine and Choline 
      Deficient Diet-Fed Rats.
PG  - 530
LID - 10.3389/fphar.2020.00530 [doi]
LID - 530
AB  - Si-Wei-Qing-Gan-Tang (SWQGT) is a Chinese medicine formula that is widely used as 
      a folk remedy of herbal tea for the treatment of chronic hepatitis, like 
      non-alcoholic steatohepatitis (NASH), around Ganzhou City (Jiangxi province, 
      China). However, the underlying mechanisms of this formula against NASH are still 
      unknown. This study aimed to explore the effect and mechanisms of SWQGT against 
      NASH. A network pharmacology approach was used to predict the potential 
      mechanisms of SWQGT against NASH. Then a rat model of NASH established by feeding 
      the methionine and choline deficient (MCD) diet was used to verify the effect and 
      mechanisms of SWQGT on NASH in vivo. SWQGT (1 g/kg/d and 3 g/kg/d) were given by 
      intragastric administration. Body weight, liver weight, serum biochemical 
      indicators, liver triglyceride and total cholesterol were all measured. Tumor 
      necrosis factor-alpha (TNF-alpha), Interleukin (IL)-1beta, IL-6 levels in the livers were 
      evaluated using ELISA. Hematoxylin and eosin (HE) and Oil Red O staining were 
      used to determine histology, while western blot was used to assess the relative 
      expression levels of the nuclear factor-kappaB (NF-kappaB) pathway- and autophagy-related 
      proteins. Functional and pathway enrichment analyses revealed that SWQGT 
      obviously influenced inflammation-related signal pathways in NASH. Furthermore, 
      in vivo experiment showed that SWQGT caused a reduction in liver weight and liver 
      index of MCD diet-fed rats. The formula also helped to reduce hepatomegaly and 
      improve pathological liver changes and hepatic steatosis. SWQGT likewise reduced 
      liver TNF-alpha, IL-1beta, and IL-6 levels and down-regulated p-NF-kappaB p65, p-p38 MAPK, 
      p-MEK1/2, p-ERK1/2, p-mTOR, and p62, while up-regulating p-ULK1 and LC3II protein 
      expression levels. SWQGT could improve NASH in MCD diet-fed rats, and this effect 
      may be associated with its down-regulation of NF-kappaB and activation of autophagy.
CI  - Copyright (c) 2020 Xiao, Liang, Ge, Qiu, Wan, Wu, Fei, Peng and Zeng.
FAU - Xiao, Lingyun
AU  - Xiao L
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan 
      University, Guangzhou, China.
FAU - Liang, Shu
AU  - Liang S
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
FAU - Ge, Lanlan
AU  - Ge L
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan 
      University, Guangzhou, China.
FAU - Qiu, Shuling
AU  - Qiu S
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
FAU - Wan, Haoqiang
AU  - Wan H
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
AD  - Department of Pathology (Longhua Branch), 2nd Clinical Medical College (Shenzhen 
      People's Hospital) of Jinan University, Shenzhen, China.
FAU - Wu, Shipin
AU  - Wu S
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
FAU - Fei, Jia
AU  - Fei J
AD  - Integrated Chinese and Western Medicine Postdoctoral Research Station, Jinan 
      University, Guangzhou, China.
FAU - Peng, Shusong
AU  - Peng S
AD  - Department of Pathology (Longhua Branch), 2nd Clinical Medical College (Shenzhen 
      People's Hospital) of Jinan University, Shenzhen, China.
FAU - Zeng, Xiaobin
AU  - Zeng X
AD  - Centre Lab of Longhua Branch and Department of Infectious Disease, 2nd Clinical 
      Medical College (Shenzhen People's Hospital) of Jinan University, Shenzhen, 
      China.
AD  - Guangdong Key Laboratory of Regional Immunity and Diseases, Shenzhen University 
      School of Medicine, Shenzhen, China.
LA  - eng
PT  - Journal Article
DEP - 20200501
PL  - Switzerland
TA  - Front Pharmacol
JT  - Frontiers in pharmacology
JID - 101548923
PMC - PMC7206618
OTO - NOTNLM
OT  - NF-kappaB
OT  - Si-Wei-Qing-Gan-Tang
OT  - autophagy
OT  - inflammation
OT  - network pharmacology
OT  - non-alcoholic steatohepatitis
EDAT- 2020/05/20 06:00
MHDA- 2020/05/20 06:01
PMCR- 2020/05/01
CRDT- 2020/05/20 06:00
PHST- 2019/09/13 00:00 [received]
PHST- 2020/04/03 00:00 [accepted]
PHST- 2020/05/20 06:00 [entrez]
PHST- 2020/05/20 06:00 [pubmed]
PHST- 2020/05/20 06:01 [medline]
PHST- 2020/05/01 00:00 [pmc-release]
AID - 10.3389/fphar.2020.00530 [doi]
PST - epublish
SO  - Front Pharmacol. 2020 May 1;11:530. doi: 10.3389/fphar.2020.00530. eCollection 
      2020.