PMID- 32430852
OWN - NLM
STAT- MEDLINE
DCOM- 20210125
LR  - 20210125
IS  - 0065-2598 (Print)
IS  - 0065-2598 (Linking)
VI  - 1292
DP  - 2020
TI  - Isopanduratin A Isolated from Boesenbergia pandurata Reduces HepG2 Hepatocellular 
      Carcinoma Cell Proliferation in Both Monolayer and Three-Dimensional Cultures.
PG  - 131-143
LID - 10.1007/5584_2020_523 [doi]
AB  - INTRODUCTION: Medicinal plants have been used for disease treatment throughout 
      history, especially in parts of Asia. Vietnam is a tropical country which 
      possesses forests with a wide diversity of plants, which have a long history of 
      being used as alternative remedies for treatment of various diseases. In this 
      study, we aimed to test the bioactivity of Boesenbergia pandurata (B. pandurata) 
      plant extract-derived compounds, including Pinostropin, Pinocembrin, Alpinetin, 
      and Isopanduratin A, on HepG2 hepatocellular carcinoma cells. METHOD: In this 
      study, several compounds from Boesenbergia pandurata plant extract, including 
      Pinostropin, Pinocembrin, Alpinetin, and Isopanduratin A, were used to evaluate 
      their antitumor effects on HepG2 hepatocellular carcinoma cells in both monolayer 
      culture condition (2D culture) and three-dimensional culture condition (3D 
      culture); HepG2 cell proliferation was assessed by Alamar Blue assay. The 
      compound which had the strongest cytotoxicity was selected for further analysis 
      by Propidium Iodide (PI) staining assay and caspase 3/7 expression assay. 
      RESULTS: The cytotoxicity assay showed that Isopanduratin A had the strongest 
      cytotoxic effect on HepG2 cells grown in both 2D culture (IC50 at 357 +/- 10 nM) 
      and 3D culture conditions (IC50 at 744 +/- 18 nM). The PI staining of HepG2 
      spheroids demonstrated that Isopanduratin A was potently cytotoxic to HepG2 cells 
      growing in spheroids (3D) and destroyed the initial shape of the spheroids. 
      Isopanduratin A was shown to induce caspase 3/7 after 30 min of incubation at the 
      concentration of 500 nM. CONCLUSION: This study demonstrated that the compound 
      Isopanduratin A isolated from B. pandurata extract has a strong inhibitory effect 
      on cell growth in 2D and 3D culture conditions. Isopanduratin A also has the 
      ability to induce apoptosis in HepG2 cells via activation of caspase 3/7. Further 
      investigations on the mechanisms of Isopanduratin A-induced effects on HepG2 
      cells, and other cancer cells, are necessary.
CN  - Sinh Truong Nguyen
CN  - Nghia Minh Do
CN  - Duyen Ho-Khanh Tran
CN  - Ngoc Bao To
CN  - Phuc Hong Vo
CN  - Mai Thi Thanh Nguyen
CN  - Nhan Trung Nguyen
CN  - Hai Xuan Nguyen
CN  - Kiet Dinh Truong
CN  - Phuc Van Pham
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Adv Exp Med Biol
JT  - Advances in experimental medicine and biology
JID - 0121103
RN  - 0 (Plant Extracts)
RN  - 0 (isopanduratin A)
SB  - IM
MH  - Apoptosis/drug effects
MH  - Carcinoma, Hepatocellular/drug therapy/*pathology
MH  - *Cell Culture Techniques
MH  - Cell Proliferation/drug effects
MH  - Hep G2 Cells
MH  - Humans
MH  - Liver Neoplasms/drug therapy/*pathology
MH  - Plant Extracts/chemistry/*isolation & purification/*pharmacology
MH  - Vietnam
MH  - Zingiberaceae/*chemistry
OTO - NOTNLM
OT  - Antitumor activity
OT  - Boesenbergia pandurata
OT  - Hepatocellular carcinoma
OT  - Ngai Bun
EDAT- 2020/05/21 06:00
MHDA- 2021/01/26 06:00
CRDT- 2020/05/21 06:00
PHST- 2020/05/21 06:00 [pubmed]
PHST- 2021/01/26 06:00 [medline]
PHST- 2020/05/21 06:00 [entrez]
AID - 10.1007/5584_2020_523 [doi]
PST - ppublish
SO  - Adv Exp Med Biol. 2020;1292:131-143. doi: 10.1007/5584_2020_523.