PMID- 32431527
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220414
IS  - 1178-7007 (Print)
IS  - 1178-7007 (Electronic)
IS  - 1178-7007 (Linking)
VI  - 13
DP  - 2020
TI  - Characteristics of Gastric Microbiota in GK Rats with Spontaneous Diabetes: A 
      Comparative Study.
PG  - 1435-1447
LID - 10.2147/DMSO.S242698 [doi]
AB  - BACKGROUND: The Goto-Kakizaki (GK) rat, developed from repeated inbreeding of 
      glucose-intolerant Wistar rats, has been widely used to explore the development 
      of spontaneous type-2 diabetes mellitus (T2DM). However, the gastric microbiota 
      of GK and Wistar rats are still unclear. This study aimed to understand the 
      gastric microbiota characteristics of GK rats by comparing it with non-diabetic 
      Wistar rats. MATERIALS AND METHODS: Male Wistar rats and GK rats were housed in 
      specific pathogen-free (SPF) environment for 12 weeks with free access to 
      sterilized food and water. Body weight and random blood glucose (BG) levels were 
      determined. At the end of the experiment, the gastric contents of the rats were 
      collected for the identification of gastric microbiota using 16S rRNA gene 
      sequencing. RESULTS: The richness of gastric microbiota in GK rats was similar to 
      that of Wistar rats (P > 0.05). The results of Shannon, Simpson, beta diversity 
      indices, and ANOSIM analysis showed that alpha and beta diversity of gastric 
      microbiota in GK rats were significantly lower than that of Wistar rats (P < 
      0.01). Firmicutes (96.0%), Proteobacteria (1.9%) and Cyanobacteria (0.8%) were 
      the dominant gastric microbiota in GK rats accounting for 72.9%, 14.7% and 10.9%, 
      respectively. Linear discriminant analysis effect size (LEfSe)  revealed  that 
      phylum Firmicutes and four genera (Anaerovibrio, Collinsella, 
      Prevotellaceae_UCG_001, and Lactobacillus) were significantly abundant in the 
      stomachs of GK rats. In contrast, seven genera (unidentified_Chloroplast, 
      Porphyromonas, Neisseria, Rubrobacter, Veillonella, Lachnospiraceae_UCG_005, and 
      unidentified_Erysipelotrichaceae) were significantly abundant in the stomachs of 
      Wistar rats. Blood glucose was positively correlated with Anaerobibrio and 
      Lactobacillus, and negatively correlated with four genera (Porphyromonas, 
      Rubrobacter, Lachnospiraceae_UCG_005, and unidentified_Erysipelotrichaceae). In 
      addition, chemoheterotrophy and fermentation were the most important functions of 
      gastric microbiota. CONCLUSION: The gastric microbiota of GK rats with 
      spontaneous T2DM showed the typical characteristics of low diversity and 
      significant enrichment of Firmicutes phylum and four genera (Anaerovibrio, 
      Collinsella, Prevotellaceae_UCG_001, and Lactobacillus) compared with gastric 
      microbiota of Wistar rats.
CI  - (c) 2020 Kang et al.
FAU - Kang, Xin
AU  - Kang X
AD  - Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, 
      People's Republic of China.
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
FAU - Zhan, Libin
AU  - Zhan L
AD  - Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, 
      People's Republic of China.
AD  - Basic Medical College, Nanjing University of Chinese Medicine, Nanjing, Jiangsu, 
      People's Republic of China.
FAU - Lu, Xiaoguang
AU  - Lu X
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
FAU - Song, Jianbo
AU  - Song J
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
FAU - Zhong, Yilong
AU  - Zhong Y
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
FAU - Wang, Yi
AU  - Wang Y
AD  - Institute of Integrative Medicine, Dalian Medical University, Dalian, Liaoning, 
      People's Republic of China.
FAU - Yang, Yilun
AU  - Yang Y
AD  - Graduate School, Zunyi Medical University, Zunyi, Guizhou, Republic of China.
FAU - Fan, Zhiwei
AU  - Fan Z
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
FAU - Jiang, Xiaozheng
AU  - Jiang X
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
FAU - Sun, Ruru
AU  - Sun R
AUID- ORCID: 0000-0001-5175-1101
AD  - Department of Emergency Medicine, Affiliated Zhongshan Hospital, Dalian 
      University, Dalian, Liaoning, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20200430
PL  - New Zealand
TA  - Diabetes Metab Syndr Obes
JT  - Diabetes, metabolic syndrome and obesity : targets and therapy
JID - 101515585
PMC - PMC7201022
OTO - NOTNLM
OT  - 16S rRNA gene sequencing
OT  - GK rat
OT  - gastric microbiota
OT  - type-2 diabetes mellitus
OT  - wistar rat
COIS- The authors report no conflicts of interest in this work.
EDAT- 2020/05/21 06:00
MHDA- 2020/05/21 06:01
PMCR- 2020/04/30
CRDT- 2020/05/21 06:00
PHST- 2020/01/25 00:00 [received]
PHST- 2020/04/07 00:00 [accepted]
PHST- 2020/05/21 06:00 [entrez]
PHST- 2020/05/21 06:00 [pubmed]
PHST- 2020/05/21 06:01 [medline]
PHST- 2020/04/30 00:00 [pmc-release]
AID - 242698 [pii]
AID - 10.2147/DMSO.S242698 [doi]
PST - epublish
SO  - Diabetes Metab Syndr Obes. 2020 Apr 30;13:1435-1447. doi: 10.2147/DMSO.S242698. 
      eCollection 2020.