PMID- 32431670
OWN - NLM
STAT- MEDLINE
DCOM- 20210519
LR  - 20210519
IS  - 1664-2392 (Print)
IS  - 1664-2392 (Electronic)
IS  - 1664-2392 (Linking)
VI  - 11
DP  - 2020
TI  - Osteogenic and Chondrogenic Potential of the Supramolecular Aggregate T-LysYal(R).
PG  - 285
LID - 10.3389/fendo.2020.00285 [doi]
LID - 285
AB  - Hard tissue regeneration represents a challenge for the Regenerative Medicine and 
      Mesenchymal stem cells (MSCs) could be a successful therapeutic strategy. 
      T-LysYal(R) (T-Lys), a new derivative of Hyaluronic Acid (HA) possessing a superior 
      stability, has already been proved efficient in repairing corneal epithelial 
      cells damaged by dry conditions in vitro. We investigated the regenerative 
      potential of T-Lys in the hard tissues bone and cartilage. We have previously 
      demonstrated that cells isolated from the tooth germ, Dental Bud Stem Cells 
      (DBSCs), differentiate into osteoblast-like cells, representing a promising 
      source of MSCs for bone regeneration. Herewith, we show that T-Lys treatment 
      stimulates the expression of typical osteoblastic markers, such as Runx-2, 
      Collagen I (Col1) and Alkaline Phosphatase (ALP), determining a higher production 
      of mineralized matrix nodules. In addition, we found that T-Lys treatment 
      positively affects alpha(V)beta(3) integrin expression, key integrin in the osteoblastic 
      commitment, leading to the formation of focal adhesions (FAs). The efficacy of 
      T-Lys was also tested on chondrogenic differentiation starting from human 
      articular chondrocytes (HACs) resulting in an increase of differentiation markers 
      and cell number.
CI  - Copyright (c) 2020 Di Benedetto, Posa, Marazzi, Kalemaj, Grassi, Lo Muzio, Comite, 
      Cavalcanti-Adam, Grassi and Mori.
FAU - Di Benedetto, Adriana
AU  - Di Benedetto A
AD  - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 
      Italy.
FAU - Posa, Francesca
AU  - Posa F
AD  - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 
      Italy.
AD  - Department of Biophysical Chemistry, Heidelberg University & Max Planck Institute 
      for Medical Research, Heidelberg, Germany.
FAU - Marazzi, Mario
AU  - Marazzi M
AD  - Struttura Semplice Tissue Therapy, Niguarda Hospital, Piazza dell'Ospedale 
      Maggiore, Milan, Italy.
FAU - Kalemaj, Zamira
AU  - Kalemaj Z
AD  - Department of Basic and Medical Sciences, Neurosciences and Sense Organs, 
      University of Bari, Bari, Italy.
FAU - Grassi, Roberta
AU  - Grassi R
AD  - Department of Biomedical Sciences, University of Sassari, Sassari, Italy.
FAU - Lo Muzio, Lorenzo
AU  - Lo Muzio L
AD  - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 
      Italy.
FAU - Comite, Mariasevera Di
AU  - Comite MD
AD  - Department of Basic and Medical Sciences, Neurosciences and Sense Organs, 
      University of Bari, Bari, Italy.
FAU - Cavalcanti-Adam, Elisabetta Ada
AU  - Cavalcanti-Adam EA
AD  - Department of Biophysical Chemistry, Heidelberg University & Max Planck Institute 
      for Medical Research, Heidelberg, Germany.
FAU - Grassi, Felice Roberto
AU  - Grassi FR
AD  - Department of Basic and Medical Sciences, Neurosciences and Sense Organs, 
      University of Bari, Bari, Italy.
FAU - Mori, Giorgio
AU  - Mori G
AD  - Department of Clinical and Experimental Medicine, University of Foggia, Foggia, 
      Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200505
PL  - Switzerland
TA  - Front Endocrinol (Lausanne)
JT  - Frontiers in endocrinology
JID - 101555782
RN  - 0 (T-LysYal)
RN  - 451W47IQ8X (Sodium Chloride)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - K3Z4F929H6 (Lysine)
RN  - QR26YLT7LT (Thymine)
SB  - IM
MH  - Cartilage, Articular/*cytology/drug effects
MH  - *Cell Differentiation
MH  - Chondrocytes/*cytology/drug effects
MH  - *Chondrogenesis
MH  - Humans
MH  - Hyaluronic Acid/chemistry
MH  - Lysine/chemistry/*pharmacology
MH  - Mesenchymal Stem Cells/*cytology/drug effects
MH  - *Osteogenesis
MH  - Sodium Chloride/chemistry/*pharmacology
MH  - Thymine/chemistry/*pharmacology
MH  - Tissue Engineering
PMC - PMC7214626
OTO - NOTNLM
OT  - bone
OT  - cartilage
OT  - focal adhesions
OT  - mesenchymal stem cells
OT  - supramolecular aggregate
OT  - tissue regeneration
EDAT- 2020/05/21 06:00
MHDA- 2021/05/20 06:00
PMCR- 2020/01/01
CRDT- 2020/05/21 06:00
PHST- 2020/01/28 00:00 [received]
PHST- 2020/04/16 00:00 [accepted]
PHST- 2020/05/21 06:00 [entrez]
PHST- 2020/05/21 06:00 [pubmed]
PHST- 2021/05/20 06:00 [medline]
PHST- 2020/01/01 00:00 [pmc-release]
AID - 10.3389/fendo.2020.00285 [doi]
PST - epublish
SO  - Front Endocrinol (Lausanne). 2020 May 5;11:285. doi: 10.3389/fendo.2020.00285. 
      eCollection 2020.