PMID- 32431798
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220414
IS  - 2008-2835 (Print)
IS  - 2008-4625 (Electronic)
IS  - 2008-2835 (Linking)
VI  - 12
IP  - 2
DP  - 2020 Apr-Jun
TI  - Gene Expression and Levels of TGF-B in PBMC Is Associated with Severity of 
      Symptoms in Chronic Heart Failure.
PG  - 132-134
AB  - BACKGROUND: TGF-beta1 is known to promote cardiac remodeling and fibrosis during 
      Congestive Heart Failure (CHF). In this study, an attempt was made to investigate 
      expression of Transforming Growth Factor beta1 (TGF-beta1) and relative expansion or 
      contraction of regulatory T-cell (Tregs) population in peripheral blood of 
      patients with Chronic Heart Failure (CHF). METHODS: Real-time PCR assay was used 
      to investigate expression and post-stimulation levels of TGF-beta1 in cell culture 
      supernatant of Peripheral Blood Mononuclear Cells (PBMC) of 42 patients with CHF 
      and 42 controls. Flow cytometry was used to identify relative counts of 
      CD4(+)CD25(+)FoxP3(+) Tregs. RESULTS: PBMCs in patients with CHF expressed higher 
      levels of TGF-beta1 compared to controls. Post-stimulation levels of TGF-beta1 
      expression were significantly higher in New York Heart Association (NYHA) 
      functional class IV patients compared to stage I patients. Tregs were 
      significantly expanded in PBMC in CHF, while the CD4(+) helper T-cells were 
      unchanged. Treg expansion was more significant in NYHA functional class I 
      patients compared to class IV patients. CONCLUSION: Expansion of Treg population 
      in CHF provides an extrinsic source for TGF-beta1 production to induce reactive 
      fibrosis and cardiac remodeling. Relative decrease in Treg population at advanced 
      stages of CHF is indicative of a loss of regulatory characteristics in these 
      cells and unopposed proinflammatory milieu.
CI  - Copyright(c) 2020 Avicenna Research Institute.
FAU - Saadati, Samaneh
AU  - Saadati S
AD  - Department of Immunology, Faculty of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Eskandari, Vajiheh
AU  - Eskandari V
AD  - Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of 
      Medical Sciences, Rasht, Iran.
FAU - Rahmani, Farzaneh
AU  - Rahmani F
AD  - Research Center for Immunodeficiencies, Pediatrics Center of Excellence, 
      Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
AD  - NeuroImaging Network (NIN), Universal Scientific Education and Research Network 
      (USERN), Tehran, Iran.
FAU - Mahmoudi, Mohammad Jafar
AU  - Mahmoudi MJ
AD  - Department of Cardiology, Amir Alam Hospital, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Rahnemoon, Zahra
AU  - Rahnemoon Z
AD  - Cardiac Heart Center, Faculty of Medicine, Tehran University of Medical Sciences, 
      Tehran, Iran.
FAU - Rahmati, Zahra
AU  - Rahmati Z
AD  - Department of Immunology, Faculty of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Gorzin, Fatemeh
AU  - Gorzin F
AD  - Department of Immunology, Faculty of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Hedayat, Mona
AU  - Hedayat M
AD  - Division of Immunology, Boston Children's Hospital, Harvard Medical School, 
      Boston, MA, USA.
AD  - Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal 
      Scientific Education and Research Network (USERN), Boston, MA, USA.
FAU - Amirzargar, Ali Akbar
AU  - Amirzargar AA
AD  - Department of Immunology, Faculty of Medicine, Tehran University of Medical 
      Sciences, Tehran, Iran.
FAU - Rezaei, Nima
AU  - Rezaei N
AD  - Research Center for Immunodeficiencies, Pediatrics Center of Excellence, 
      Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran.
AD  - Network of Immunity in Infection, Malignancy and Autoimmunity (NIIMA), Universal 
      Scientific Education and Research Network (USERN), Boston, MA, USA.
LA  - eng
PT  - Journal Article
PL  - Iran
TA  - Avicenna J Med Biotechnol
JT  - Avicenna journal of medical biotechnology
JID - 101511065
PMC - PMC7229453
OTO - NOTNLM
OT  - Cell culture techniques
OT  - Chronic heart failure
OT  - T-lymphocytes
OT  - Transforming growth factor beta1
EDAT- 2020/05/21 06:00
MHDA- 2020/05/21 06:01
PMCR- 2020/04/01
CRDT- 2020/05/21 06:00
PHST- 2020/05/21 06:00 [entrez]
PHST- 2020/05/21 06:00 [pubmed]
PHST- 2020/05/21 06:01 [medline]
PHST- 2020/04/01 00:00 [pmc-release]
AID - AJMB-12-132 [pii]
PST - ppublish
SO  - Avicenna J Med Biotechnol. 2020 Apr-Jun;12(2):132-134.