PMID- 32432315
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20210330
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 40
IP  - 6
DP  - 2020 Jun 26
TI  - Prevalence and antibiotic resistance of Listeria monocytogenes in camel meat.
LID - 10.1042/BSR20201062 [doi]
LID - BSR20201062
AB  - In the present study, a total of 50 raw camel meat samples were analyzed for the 
      presence of Listeria monocytogenes. The isolates were characterized via 
      morphological and culture analyses; identification of isolates was confirmed by 
      polymerase chain reaction (PCR) and sequencing of the listeriolysin O gene. The 
      API Listeria system was used for further chemical identification and verification 
      of the strains. L. monocytogenes was identified in eight raw camel meat samples, 
      which was the highest incidence (16%) of contamination, followed by L. seeligeri 
      3(6%), L. innocua and L. welshimeri 2 (2% each), and L. grayi 1 (1%). According 
      to Basic Local Alignment Search Tool (BLAST) analysis, isolated strains that were 
      positive for the listeriolysin O gene were >99% similar to the published database 
      sequences for L. monocytogenes strain LM850658 (sequence ID: CP009242.1). We 
      studied the antibiotic resistance profile of the L. monocytogenes strains with 
      common antibiotics used to treat human listeriosis and demonstrated that almost 
      all strains tested were susceptible to the antibiotics.
CI  - (c) 2020 The Author(s).
FAU - Yehia, Hany M
AU  - Yehia HM
AD  - Food Science and Nutrition Department, College of Food and Agricultural Sciences, 
      King Saud University, Riyadh 11451, Saudi Arabia.
AD  - Food Science and Nutrition Department, Faculty of Home Economics, Helwan 
      University, Cairo, Egypt.
FAU - Elkhadragy, Manal F
AU  - Elkhadragy MF
AD  - Biology Department, Faculty of Science, Princess Nourah bint Abdulrahman 
      University, Riyadh 11671, Saudi Arabia.
AD  - Zoology Department, Faculty of Science, Helwan University, Cairo 11790, Egypt.
FAU - Aljahani, Amani H
AU  - Aljahani AH
AD  - Department of Physical Sport Science, Nutrition and Food Science (PhD), Princess 
      Nourah bint Abdulrahman University, Riyadh, P.O. Box 84428, Riyadh 11671, Saudi 
      Arabia.
FAU - Alarjani, Khaloud M
AU  - Alarjani KM
AD  - Department of Botany and Microbiology, College of Science, King Saud University, 
      Riyadh 11451, Saudi Arabia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (Bacterial Toxins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Hemolysin Proteins)
RN  - R06ZRQ1YX9 (hlyA protein, Listeria monocytogenes)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*pharmacology
MH  - Bacterial Toxins/genetics
MH  - Camelus/*microbiology
MH  - DNA, Bacterial/genetics
MH  - *Drug Resistance, Multiple, Bacterial
MH  - *Food Microbiology
MH  - Foodborne Diseases/microbiology/*prevention & control
MH  - Heat-Shock Proteins/genetics
MH  - Hemolysin Proteins/genetics
MH  - Listeria monocytogenes/*drug effects/genetics/isolation & purification
MH  - Listeriosis/microbiology/*prevention & control/transmission
MH  - Meat/*microbiology
MH  - Microbial Sensitivity Tests
PMC - PMC7281775
OTO - NOTNLM
OT  - Listeria monocytogenes
OT  - antimicrobial resistance
OT  - listeriolysin O (hly) gene
OT  - raw camel meat
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2020/05/21 06:00
MHDA- 2021/03/31 06:00
PMCR- 2020/06/04
CRDT- 2020/05/21 06:00
PHST- 2020/02/26 00:00 [received]
PHST- 2020/05/17 00:00 [revised]
PHST- 2020/05/22 00:00 [accepted]
PHST- 2020/05/21 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
PHST- 2020/05/21 06:00 [entrez]
PHST- 2020/06/04 00:00 [pmc-release]
AID - 224889 [pii]
AID - BSR20201062 [pii]
AID - 10.1042/BSR20201062 [doi]
PST - ppublish
SO  - Biosci Rep. 2020 Jun 26;40(6):BSR20201062. doi: 10.1042/BSR20201062.