PMID- 32432318
OWN - NLM
STAT- MEDLINE
DCOM- 20210330
LR  - 20240328
IS  - 1573-4935 (Electronic)
IS  - 0144-8463 (Print)
IS  - 0144-8463 (Linking)
VI  - 40
IP  - 6
DP  - 2020 Jun 26
TI  - Molecular mechanism of targeted inhibition of HMGA2 via miRNAlet-7a in 
      proliferation and metastasis of laryngeal squamous cell carcinoma.
LID - 10.1042/BSR20193788 [doi]
LID - BSR20193788
AB  - MiRNAlet-7a is associated with the tumorigenesis of laryngeal squamous cell 
      carcinoma (LSCC). Our study was designed to infer whether let-7a targets 
      high-mobility AT-hook 2 (HMGA2) and suppresses laryngeal carcinoma cell 
      proliferation, invasion, and migration. The expression levels of let-7a and HMGA2 
      were measured in 30 LSCC clinical specimens by qRT-PCR and their correlation was 
      analyzed. Cell model and mice xenograft model with or without let-7a 
      overexpression were constructed to evaluate the effects of let-7a on LSCC. 
      Moreover, luciferase assay was performed to reveal the interaction between let-7a 
      and HMGA2, which was further verified in xenograft. Let-7a was significantly 
      down-regulated and HMGA2 was up-regulated in LSCC tissues compared with normal 
      tissues (P<0.05), both of which were significantly correlated with TNM stage and 
      lymph node metastases of LSCC patients (P<0.05). We also observed a negative 
      correlation between let-7a and HMGA2 expression in LSCC samples (r = -0.642, 
      P<0.05). In vitro and in vivo experiments demonstrated that let-7a overexpression 
      could inhibit cell proliferation and tumor growth of LSCC and simultaneously 
      down-regulate the expression of HMGA2. Moreover, the regulation of HMGA2 by 
      let-7a was also proved by luciferase assay. Our results revealed that let-7a 
      promotes development and progression of LSCC through inhibiting the expression of 
      HMGA2. Therefore, let-7a may thus be a potential diagnostic biomarker and 
      therapeutic target for treating LSCC.
CI  - (c) 2020 The Author(s).
FAU - Ma, Li-Juan
AU  - Ma LJ
AD  - Department of Otolaryngology Head/Neck Surgery, Hunan Provincial People's 
      Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 
      410005, P. R. China.
FAU - Wu, Jun
AU  - Wu J
AD  - Department of Otolaryngology Head/Neck Surgery, Hunan Provincial People's 
      Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 
      410005, P. R. China.
FAU - Zhou, En
AU  - Zhou E
AD  - Department of Otolaryngology Head/Neck Surgery, Hunan Provincial People's 
      Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 
      410005, P. R. China.
FAU - Yin, Juan
AU  - Yin J
AD  - Department of Otolaryngology Head/Neck Surgery, Hunan Provincial People's 
      Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 
      410005, P. R. China.
FAU - Xiao, Xu-Ping
AU  - Xiao XP
AD  - Department of Otolaryngology Head/Neck Surgery, Hunan Provincial People's 
      Hospital, The First Affiliated Hospital of Hunan Normal University, Changsha 
      410005, P. R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biosci Rep
JT  - Bioscience reports
JID - 8102797
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (HMGA2 Protein)
RN  - 0 (HMGA2 protein, human)
RN  - 0 (MicroRNAs)
RN  - 0 (mirnlet7 microRNA, human)
SB  - IM
MH  - Animals
MH  - Biomarkers, Tumor/genetics/*metabolism
MH  - Cell Line, Tumor
MH  - *Cell Movement
MH  - *Cell Proliferation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HMGA2 Protein/genetics/*metabolism
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*metabolism/pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/genetics/*metabolism
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Signal Transduction
MH  - Squamous Cell Carcinoma of Head and Neck/genetics/*metabolism/secondary
MH  - Tumor Burden
PMC - PMC7269914
OTO - NOTNLM
OT  - HMGA2
OT  - MiRNAlet-7a
OT  - cell apoptosis
OT  - cell proliferation
OT  - laryngeal squamous cell carcinoma
COIS- The authors declare that there are no competing interests associated with the 
      manuscript.
EDAT- 2020/05/21 06:00
MHDA- 2021/03/31 06:00
PMCR- 2020/06/03
CRDT- 2020/05/21 06:00
PHST- 2019/10/30 00:00 [received]
PHST- 2020/05/14 00:00 [revised]
PHST- 2020/05/15 00:00 [accepted]
PHST- 2020/05/21 06:00 [pubmed]
PHST- 2021/03/31 06:00 [medline]
PHST- 2020/05/21 06:00 [entrez]
PHST- 2020/06/03 00:00 [pmc-release]
AID - 224886 [pii]
AID - BSR20193788 [pii]
AID - 10.1042/BSR20193788 [doi]
PST - ppublish
SO  - Biosci Rep. 2020 Jun 26;40(6):BSR20193788. doi: 10.1042/BSR20193788.