PMID- 32435051 OWN - NLM STAT- MEDLINE DCOM- 20210218 LR - 20210218 IS - 1643-3750 (Electronic) IS - 1234-1010 (Print) IS - 1234-1010 (Linking) VI - 26 DP - 2020 May 21 TI - Bioinformatics Analysis Identifies the Estrogen Receptor 1 (ESR1) Gene and hsa-miR-26a-5p as Potential Prognostic Biomarkers in Patients with Intrahepatic Cholangiocarcinoma. PG - e921815 LID - 10.12659/MSM.921815 [doi] AB - BACKGROUND Intrahepatic cholangiocarcinoma arises from the epithelial cells of the bile ducts and is associated with poor prognosis. This study aimed to use bioinformatics analysis to identify molecular biomarkers of intrahepatic cholangiocarcinoma and their potential mechanisms. MATERIAL AND METHODS MicroRNA (miRNA) and mRNA microarrays from GSE53870 and GSE32879 were downloaded from the Gene Expression Omnibus (GEO) database. Differentially expressed miRNAs (DEMs) associated with prognosis were identified using limma software and Kaplan-Meier survival analysis. Predictive target genes of the DEMs were identified using miRWalk, miRTarBase, miRDB, and TargetScan databases of miRNA-binding sites and targets. Target genes underwent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Hub genes were analyzed by constructing the protein-protein interaction (PPI) network using Cytoscape. DEMs validated the hub genes, followed by construction of the miRNA-gene regulatory network. RESULTS Twenty-five DEMs were identified. Fifteen DEMs were upregulated, and ten were down-regulated. Kaplan-Meier survival analysis identified seven upregulated DEMs and nine down-regulated DEMs that were associated with the overall survival (OS), and 130 target genes were selected. GO analysis showed that target genes were mainly enriched for metabolism and development processes. KEGG analysis showed that target genes were mainly enriched for cancer processes and some signaling pathways. Fourteen hub genes identified from the PPI network were associated with the regulation of cell proliferation. The overlap between hub genes and DEMs identified the estrogen receptor 1 (ESR1) gene and hsa-miR-26a-5p. CONCLUSIONS Bioinformatics analysis identified ESR1 and hsa-miR-26a-5p as potential prognostic biomarkers for intrahepatic cholangiocarcinoma. FAU - Qin, Xianzheng AU - Qin X AD - Queen Mary School of Nanchang University, Nanchang, Jiangxi, China (mainland). FAU - Song, Yuning AU - Song Y AD - Queen Mary School of Nanchang University, Nanchang, Jiangxi, China (mainland). LA - eng PT - Journal Article DEP - 20200521 PL - United States TA - Med Sci Monit JT - Medical science monitor : international medical journal of experimental and clinical research JID - 9609063 RN - 0 (Biomarkers, Tumor) RN - 0 (Estrogen Receptor alpha) RN - 0 (MIRN26A microRNA, human) RN - 0 (MicroRNAs) RN - 0 (RNA, Messenger) SB - IM MH - Bile Duct Neoplasms/*genetics/metabolism MH - Biomarkers, Tumor/genetics/metabolism MH - Cholangiocarcinoma/*genetics/metabolism MH - Computational Biology/methods MH - Databases, Genetic MH - Estrogen Receptor alpha/*genetics/metabolism MH - Gene Expression Profiling/methods MH - Gene Ontology MH - Gene Regulatory Networks MH - Humans MH - MicroRNAs/*genetics/metabolism MH - Prognosis MH - Protein Interaction Maps MH - RNA, Messenger/genetics MH - Signal Transduction MH - Software PMC - PMC7257878 COIS- Conflict of interests None. EDAT- 2020/05/22 06:00 MHDA- 2021/02/20 06:00 PMCR- 2020/05/21 CRDT- 2020/05/22 06:00 PHST- 2020/05/22 06:00 [entrez] PHST- 2020/05/22 06:00 [pubmed] PHST- 2021/02/20 06:00 [medline] PHST- 2020/05/21 00:00 [pmc-release] AID - 921815 [pii] AID - 10.12659/MSM.921815 [doi] PST - epublish SO - Med Sci Monit. 2020 May 21;26:e921815. doi: 10.12659/MSM.921815.