PMID- 32437400
OWN - NLM
STAT- MEDLINE
DCOM- 20200812
LR  - 20200812
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 15
IP  - 5
DP  - 2020
TI  - Interferon regulatory factor 7 mediates obesity-associated MCP-1 transcription.
PG  - e0233390
LID - 10.1371/journal.pone.0233390 [doi]
LID - e0233390
AB  - Hypertrophy, associated with adipocyte dysfunction, causes increased 
      pro-inflammatory adipokine, and abnormal glucose and lipid metabolism, leading to 
      insulin resistance and obesity-related-health problems. By combining DNA 
      microarray and genomic data analyses to predict DNA binding motifs, we identified 
      the transcription factor Interferon Regulatory Factor 7 (IRF7) as a possible 
      regulator of genes related to adipocyte hypertrophy. To investigate the role of 
      IRF7 in adipocytes, we examined gene expression patterns in 3T3-L1 cells infected 
      with a retrovirus carrying the IRF7 gene and found that enforced IRF7 expression 
      induced the expression of monocyte chemoattractant protein-1 (MCP-1), a key 
      initial adipokine in the chronic inflammation of obesity. CRISPR/Cas9 
      mediated-suppression of IRF7 significantly reduced MCP-1 mRNA. Luciferase assays, 
      chromatin immunoprecipitation PCR analysis and gel shift assay showed that IRF7 
      transactivates the MCP-1 gene by binding to its proximal Interferon Stimulation 
      Response Element (ISRE), a putative IRF7 binding motif. IRF7 knockout mice 
      exhibited lower expression of MCP-1 in epidydimal white adipose tissue under 
      high-fat feeding conditions, suggesting the transcription factor is 
      physiologically important for inducing MCP-1. Taken together, our results suggest 
      that IRF7 transactivates MCP-1 mRNA in adipocytes, and it may be involved in the 
      adipose tissue inflammation associated with obesity.
FAU - Kuroda, Masashi
AU  - Kuroda M
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Nishiguchi, Misa
AU  - Nishiguchi M
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Ugawa, Naho
AU  - Ugawa N
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Ishikawa, Etsuko
AU  - Ishikawa E
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Kawabata, Yasuyo
AU  - Kawabata Y
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Okamoto, Saya
AU  - Okamoto S
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Sasaki, Waka
AU  - Sasaki W
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Miyatake, Yumiko
AU  - Miyatake Y
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Sebe, Mayu
AU  - Sebe M
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Masumoto, Saeko
AU  - Masumoto S
AD  - Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima-city, 
      Fukushima, Japan.
FAU - Tsutsumi, Rie
AU  - Tsutsumi R
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
FAU - Harada, Nagakatsu
AU  - Harada N
AD  - Department of Health and Nutrition, Faculty of Nursing and Nutrition, The 
      University of Shimane, Izumo-city, Shimane, Japan.
FAU - Sakaue, Hiroshi
AU  - Sakaue H
AUID- ORCID: 0000-0002-2468-2363
AD  - Department of Nutrition and Metabolism, Institute of Biomedical Sciences, 
      Tokushima University Graduate School, Tokushima-city, Tokushima, Japan.
AD  - Diabetes Therapeutics and Research Center, Tokushima University, Tokushima-city, 
      Tokushima, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20200521
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interferon Regulatory Factor-7)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Adipose Tissue, White/metabolism
MH  - Animals
MH  - Chemokine CCL2/*genetics/metabolism
MH  - Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - Interferon Regulatory Factor-7/*genetics/metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - Obesity/*genetics/metabolism
MH  - Promoter Regions, Genetic
PMC - PMC7241760
COIS- The authors have declared that no competing interests exist.
EDAT- 2020/05/22 06:00
MHDA- 2020/08/13 06:00
PMCR- 2020/05/21
CRDT- 2020/05/22 06:00
PHST- 2019/12/05 00:00 [received]
PHST- 2020/05/04 00:00 [accepted]
PHST- 2020/05/22 06:00 [entrez]
PHST- 2020/05/22 06:00 [pubmed]
PHST- 2020/08/13 06:00 [medline]
PHST- 2020/05/21 00:00 [pmc-release]
AID - PONE-D-19-33716 [pii]
AID - 10.1371/journal.pone.0233390 [doi]
PST - epublish
SO  - PLoS One. 2020 May 21;15(5):e0233390. doi: 10.1371/journal.pone.0233390. 
      eCollection 2020.