PMID- 32437893
OWN - NLM
STAT- MEDLINE
DCOM- 20210219
LR  - 20210219
IS  - 1873-6351 (Electronic)
IS  - 0278-6915 (Linking)
VI  - 141
DP  - 2020 Jul
TI  - Prenatal ethanol exposure-induced hypothalamic an imbalance of 
      glutamatergic/GABAergic projections and low functional expression in male 
      offspring rats.
PG  - 111419
LID - S0278-6915(20)30309-4 [pii]
LID - 10.1016/j.fct.2020.111419 [doi]
AB  - This study was designed to demonstrate that prenatal ethanol exposure (PEE) can 
      induce low functional expression of the hypothalamus in male offspring rats and 
      explore the underlying mechanism. Pregnant rats were administered 4 g/kg ethanol 
      or normal saline by oral gavage each day from gestational day (GD) 9 to GD20. 
      Male GD20 foetuses and postnatal day 120 adult offspring rats were sacrificed 
      under anaesthesia. Hypothalamic cells from male GD20~postnatal day (PD) 7 rats 
      were treated with different doses of corticosterone and the glucocorticoid 
      receptor (GR) antagonist mifepristone for 5 days. In this study, we found that 
      PEE-induced overexposure of maternal glucocorticoids enhanced the expression of 
      L-glutamic acid decarboxylase (GAD) 67 in the hypothalamic paraventricular 
      nucleus (PVN) by activating the glucocorticoid metabolic activation system, 
      further inducing the conversion of glutamate to L-gamma-aminobutyric acid (GABA) 
      and developmental imbalance of glutamatergic/GABAergic projections to the PVN. 
      The imbalance change was maintained until after birth, resulting in the 
      inhibition of parvocellular neurons and low functional expression of the 
      hypothalamus in PEE offspring rats. Our study indicated that low functional 
      expression of the hypothalamus in male PEE offspring rats was associated with 
      developmental programming of an imbalance of glutamatergic/GABAergic projections 
      to the PVN.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Lu, Juan
AU  - Lu J
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China; Department of Orthopedics, Gansu Provincial 
      Hospital of TCM Affiliated to Gansu University of Chinese Medicine, Gansu, 
      730050, China.
FAU - Li, Qiang
AU  - Li Q
AD  - Department of Orthopedics, Gansu Provincial Hospital of TCM Affiliated to Gansu 
      University of Chinese Medicine, Gansu, 730050, China.
FAU - Ma, Guoqin
AU  - Ma G
AD  - Department of Orthopedics, Gansu Provincial Hospital of TCM Affiliated to Gansu 
      University of Chinese Medicine, Gansu, 730050, China.
FAU - Hong, Chenghao
AU  - Hong C
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China.
FAU - Zhang, Wenqian
AU  - Zhang W
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China.
FAU - Wang, Yuxia
AU  - Wang Y
AD  - Department of Obstetrics and Gynecology, The First Affiliated Hospital of Jinan 
      University, Guangzhou, 510630, China. Electronic address: sunneywang@126.com.
FAU - Wang, Hui
AU  - Wang H
AD  - Department of Pharmacology, Wuhan University School of Basic Medical Sciences, 
      Wuhan, 430071, China; Hubei Provincial Key Laboratory of Developmentally 
      Originated Disease, Wuhan, 430071, China. Electronic address: 
      wanghui19@whu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20200511
PL  - England
TA  - Food Chem Toxicol
JT  - Food and chemical toxicology : an international journal published for the British 
      Industrial Biological Research Association
JID - 8207483
RN  - 3K9958V90M (Ethanol)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
RN  - 9002-60-2 (Adrenocorticotropic Hormone)
RN  - EC 4.1.1.15 (Glutamate Decarboxylase)
RN  - EC 4.1.1.15 (glutamate decarboxylase 1)
RN  - W980KJ009P (Corticosterone)
SB  - IM
MH  - Adrenocorticotropic Hormone/blood
MH  - Animals
MH  - Corticosterone/blood
MH  - Ethanol/*toxicity
MH  - Female
MH  - Fetal Weight
MH  - GABAergic Neurons/metabolism
MH  - Glutamate Decarboxylase/metabolism
MH  - Glutamic Acid/*metabolism
MH  - Male
MH  - Paraventricular Hypothalamic Nucleus/*drug effects/metabolism
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Wistar
MH  - gamma-Aminobutyric Acid/*metabolism
OTO - NOTNLM
OT  - Developmental programming
OT  - Ethanol
OT  - Glucocorticoid metabolic activation system
OT  - Glutamatergic/GABAergic projections
OT  - Hypothalamus
OT  - Low functional expression
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/05/22 06:00
MHDA- 2021/02/20 06:00
CRDT- 2020/05/22 06:00
PHST- 2020/01/25 00:00 [received]
PHST- 2020/05/05 00:00 [revised]
PHST- 2020/05/05 00:00 [accepted]
PHST- 2020/05/22 06:00 [pubmed]
PHST- 2021/02/20 06:00 [medline]
PHST- 2020/05/22 06:00 [entrez]
AID - S0278-6915(20)30309-4 [pii]
AID - 10.1016/j.fct.2020.111419 [doi]
PST - ppublish
SO  - Food Chem Toxicol. 2020 Jul;141:111419. doi: 10.1016/j.fct.2020.111419. Epub 2020 
      May 11.