PMID- 32438234
OWN - NLM
STAT- MEDLINE
DCOM- 20200819
LR  - 20200819
IS  - 1873-7072 (Electronic)
IS  - 0308-8146 (Linking)
VI  - 326
DP  - 2020 Oct 1
TI  - Fluorescence immunoassay for multiplex detection of organophosphate pesticides in 
      agro-products based on signal amplification of gold nanoparticles and 
      oligonucleotides.
PG  - 126813
LID - S0308-8146(20)30675-0 [pii]
LID - 10.1016/j.foodchem.2020.126813 [doi]
AB  - Herein, we developed a multi-analyte fluorescence immunoassay for detection of 
      three organophosphate pesticides (triazophos, parathion, and chlorpyrifos) in 
      various agro-products (rice, wheat, cucumber, cabbage, and apple) using 
      fluorescently labeled oligonucleotide and gold nanoparticle (AuNP) signal 
      amplification technology. The AuNP probes for the three analytes were constructed 
      by simultaneously modifying the corresponding antibodies and fluorescently 
      labeled oligonucleotides on the probe surface. Three fluorophores (6-FAM, Cy3, 
      and Texas red) with high fluorescence intensity and little overlap of 
      excitation/emission wavelengths were selected. The method showed satisfactory 
      linearity for triazophos, parathion, and chlorpyrifos in the ranges of 0.01-20, 
      0.05-50, and 0.5-1000 mug/L, respectively. For the 3 analytes, the limits of 
      detection (LODs) were 0.007, 0.009, and 0.087 mug/L, respectively. The average 
      recoveries were 77.7-113.6%, with relative standard deviations (RSDs) of 
      7.1-17.1% in various food matrices. The proposed method offers great potential in 
      food safety surveillance, and could be used as well as a reference for 
      multi-residue analysis of other small-molecule contaminants.
CI  - Copyright (c) 2020 Elsevier Ltd. All rights reserved.
FAU - Zhang, Chan
AU  - Zhang C
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Key 
      Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of 
      Agriculture, Beijing 100081, PR China.
FAU - Jiang, Zejun
AU  - Jiang Z
AD  - College of Life Sciences, China Jiliang University, Hangzhou 310018, PR China.
FAU - Jin, Maojun
AU  - Jin M
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Key 
      Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of 
      Agriculture, Beijing 100081, PR China. Electronic address: katonking@163.com.
FAU - Du, Pengfei
AU  - Du P
AD  - Institute of Agro-Food Science and Technology, Shandong Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Products Processing Technology of Shandong 
      Province, Key Laboratory of Novel Food Resources Processing, Ministry of 
      Agriculture, Jinan 250100, PR China.
FAU - Chen, Ge
AU  - Chen G
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Key 
      Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of 
      Agriculture, Beijing 100081, PR China.
FAU - Cui, Xueyan
AU  - Cui X
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Key 
      Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of 
      Agriculture, Beijing 100081, PR China.
FAU - Zhang, Yudan
AU  - Zhang Y
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Key 
      Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of 
      Agriculture, Beijing 100081, PR China.
FAU - Qin, Guoxin
AU  - Qin G
AD  - Agro-products Quality Safety and Testing Technology Research Institute, Guangxi 
      Academy of Agricultural Sciences, Nanning 530007, PR China.
FAU - Yan, Feiyan
AU  - Yan F
AD  - Agro-products Quality Safety and Testing Technology Research Institute, Guangxi 
      Academy of Agricultural Sciences, Nanning 530007, PR China.
FAU - Abd El-Aty, A M
AU  - Abd El-Aty AM
AD  - State Key Laboratory of Biobased Material and Green Papermaking, College of Food 
      Science and Engineering, Qilu University of Technology, Shandong Academy of 
      Science, Jinan 250353, PR China; Department of Pharmacology, Faculty of 
      Veterinary Medicine, Cairo University, 12211 Giza, Egypt; Department of Medical 
      Pharmacology, Medical Faculty, Ataturk University, 25240 Erzurum, Turkey. 
      Electronic address: abdelaty44@hotmail.com.
FAU - Hacimuftuoglu, Ahmet
AU  - Hacimuftuoglu A
AD  - Department of Medical Pharmacology, Medical Faculty, Ataturk University, 25240 
      Erzurum, Turkey.
FAU - Wang, Jing
AU  - Wang J
AD  - Institute of Quality Standard and Testing Technology for Agro-Products, Key 
      Laboratory of Agro-Product Quality and Safety, Chinese Academy of Agricultural 
      Sciences, Key Laboratory of Agro-Product Quality and Safety, Ministry of 
      Agriculture, Beijing 100081, PR China. Electronic address: w_jing2001@126.com.
LA  - eng
PT  - Journal Article
DEP - 20200415
PL  - England
TA  - Food Chem
JT  - Food chemistry
JID - 7702639
RN  - 0 (Insecticides)
RN  - 0 (Oligonucleotides)
RN  - 0 (Organothiophosphates)
RN  - 0 (Pesticides)
RN  - 0 (Triazoles)
RN  - 6099J8L0EE (triazophos)
RN  - 61G466064D (Parathion)
RN  - 7440-57-5 (Gold)
RN  - JCS58I644W (Chlorpyrifos)
SB  - IM
MH  - Chlorpyrifos/analysis
MH  - Fluorescence
MH  - Food Analysis
MH  - Gold/*chemistry
MH  - Immunoassay/methods
MH  - Insecticides/*analysis
MH  - Limit of Detection
MH  - Metal Nanoparticles/*chemistry
MH  - Oligonucleotides/*chemistry
MH  - Organothiophosphates/analysis
MH  - Parathion/analysis
MH  - Pesticides/analysis
MH  - Triazoles/analysis
OTO - NOTNLM
OT  - Chlorpyrifos (PubChem CID: 2730)
OT  - Dithiothreitol (PubChem CID: 446094)
OT  - Fluorescence immunoassay
OT  - Gold nanoparticle
OT  - Oligonucleotide
OT  - Organophosphate pesticides
OT  - Parathion (PubChem CID: 991)
OT  - Triazophos (PubChem CID: 32184)
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2020/05/22 06:00
MHDA- 2020/08/20 06:00
CRDT- 2020/05/22 06:00
PHST- 2019/04/14 00:00 [received]
PHST- 2020/02/26 00:00 [revised]
PHST- 2020/04/13 00:00 [accepted]
PHST- 2020/05/22 06:00 [pubmed]
PHST- 2020/08/20 06:00 [medline]
PHST- 2020/05/22 06:00 [entrez]
AID - S0308-8146(20)30675-0 [pii]
AID - 10.1016/j.foodchem.2020.126813 [doi]
PST - ppublish
SO  - Food Chem. 2020 Oct 1;326:126813. doi: 10.1016/j.foodchem.2020.126813. Epub 2020 
      Apr 15.