PMID- 32438355
OWN - NLM
STAT- MEDLINE
DCOM- 20210830
LR  - 20210830
IS  - 1748-605X (Electronic)
IS  - 1748-6041 (Linking)
VI  - 15
IP  - 5
DP  - 2020 Aug 21
TI  - Development of osteogenic chitosan/alginate scaffolds reinforced with 
      silicocarnotite containing apatitic fibers.
PG  - 055020
LID - 10.1088/1748-605X/ab954f [doi]
AB  - Porous composite scaffolds of chitosan-alginate (CH-AL) reinforced by biphasic 
      calcium phosphate fibers containing silicon (Si) were prepared using the 
      freeze-drying method. The fibers were synthesized using a homogenous 
      precipitation method with differing reaction times and were characterized by XRD, 
      FTIR, SEM, and ICP-OES. Fibers produced with no Si incorporation using two 
      different reaction times of 4 d and 8 d comprised two phases of hydroxyapatite 
      ( approximately 93-96 wt%) and beta-tricalcium phosphate (beta-TCP). No new phases were observed by 
      adding 0.8 wt% of Si during 4 d of precipitation. However, the addition of Si to 
      fibers synthesized within 8 d under reflux conditions produced biphasic fibers 
      with 1.9 wt% Si which consisted of a new phase of silicocarnotite ( approximately 94 wt%) 
      associated with the beta-TCP phase. The whisker-like fibers were 10-200 microm in length 
      and 0.2-5 microm in width. The physicochemical, mechanical, and biological properties 
      of composite scaffolds fabricated by adding different fiber contents and types 
      were investigated. The scaffolds exhibited favorable microstructures with a high 
      porosity (66-88%) and the interconnected pores varied in size between 40 and 250 
      microm. Scaffolds containing silicocarnotite showed a significant improvement in 
      their mechanical properties and in vitro bioactivity (using SBF testing and 
      characterization of the apatite layer by ATR-FTIR and SEM/EDS) as well as 
      proliferation, mineralization and adhesion of MG63 cells, when evaluated by MTT 
      assay, alkaline phosphatase, and SEM. Scaffolds reinforced with silicocarnotite 
      fibers also exhibited better mechanical properties and water uptake, compared to 
      ones containing incorporated fibers made of Si. Composite scaffolds reinforced by 
      50 wt% fibers precipitated after 8 d were superior in terms of their mechanical 
      properties and achieved a compressive strength and modulus of 272 kPa and 4.9 
      MPa, respectively, which is 400% greater than CH-AL scaffolds. The results 
      indicate that the addition of Si into biphasic fibers, leading to the formation 
      of silicocarnotite, makes silicocarnotite a potential candidate for the bioactive 
      reinforcement of composite scaffolds for bone tissue engineering.
FAU - Karimi, Mahsa
AU  - Karimi M
AD  - Biomaterials Laboratory, Department of Life Science Engineering, Faculty of New 
      Sciences and Technologies, University of Tehran, Tehran, Iran.
FAU - Mesgar, Abdorreza S
AU  - Mesgar AS
FAU - Mohammadi, Zahra
AU  - Mohammadi Z
LA  - eng
PT  - Journal Article
DEP - 20200821
PL  - England
TA  - Biomed Mater
JT  - Biomedical materials (Bristol, England)
JID - 101285195
RN  - 0 (Alginates)
RN  - 0 (Apatites)
RN  - 0 (Biocompatible Materials)
RN  - 0 (Calcium Phosphates)
RN  - 0 (Polymers)
RN  - 0 (Silicates)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 0 (beta-tricalcium phosphate)
RN  - 0 (silicocarnotite)
RN  - 9012-76-4 (Chitosan)
RN  - 97Z1WI3NDX (calcium phosphate)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Alginates/*chemistry
MH  - Apatites/*chemistry
MH  - Biocompatible Materials/chemistry
MH  - Calcium Phosphates/*chemistry
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Chitosan/*chemistry
MH  - Compressive Strength
MH  - Humans
MH  - Materials Testing
MH  - Microscopy, Electron, Scanning
MH  - *Osteogenesis
MH  - Polymers/chemistry
MH  - Porosity
MH  - Regeneration
MH  - Silicates/*chemistry
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Stress, Mechanical
MH  - Tetrazolium Salts/chemistry
MH  - Thiazoles/chemistry
MH  - Tissue Engineering/methods
MH  - Tissue Scaffolds/chemistry
MH  - X-Ray Diffraction
EDAT- 2020/05/22 06:00
MHDA- 2021/08/31 06:00
CRDT- 2020/05/22 06:00
PHST- 2020/05/22 06:00 [pubmed]
PHST- 2021/08/31 06:00 [medline]
PHST- 2020/05/22 06:00 [entrez]
AID - 10.1088/1748-605X/ab954f [doi]
PST - epublish
SO  - Biomed Mater. 2020 Aug 21;15(5):055020. doi: 10.1088/1748-605X/ab954f.