PMID- 32439252
OWN - NLM
STAT- Publisher
LR  - 20240227
IS  - 2173-5751 (Electronic)
IS  - 2173-5751 (Linking)
DP  - 2020 May 18
TI  - miR-320c Regulates SERPINA1 Expression and Is Induced in Patients With Pulmonary 
      Disease.
LID - S0300-2896(20)30084-3 [pii]
LID - 10.1016/j.arbres.2020.03.006 [doi]
AB  - INTRODUCTION: Alpha-1 antitrypsin deficiency (AATD) is a genetic condition 
      resulting in lung and liver disease with a great clinical variability. MicroRNAs 
      have been identified as disease modifiers; therefore miRNA deregulation could 
      play an important role in disease heterogeneity. Members of miR-320 family are 
      involved in regulating of multiple processes including inflammation, and have 
      potential specific binding sites in the 3'UTR region of SERPINA1 gene. In this 
      study we explore the involvement of miR-320c, a member of this family, in this 
      disease. METHODS: Firstly in vitro studies were carried out to demonstrate 
      regulation of SERPINA1 gene by miR-320. Furthermore, the expression of miR-320c 
      was analyzed in the blood of 98 individuals with different AAT serum levels by 
      using quantitative PCR and expression was correlated to clinical parameters of 
      the patients. Finally, HL60 cells were used to analyze induction of miR-320c in 
      inflammatory conditions. RESULTS: Overexpression of miR-320 members in human 
      HepG2 cells led to inhibition of SERPINA1 expression. Analysis of miR-320c 
      expression in patient's samples revealed significantly increased expression of 
      miR-320c in individuals with pulmonary disease. Additionally, HL60 cells treated 
      with the pro-inflammatory factor lipopolysaccharide (LPS) showed increase in 
      miR-320c expression, suggesting that miR-320c responds to inflammation. 
      CONCLUSION: Our findings demonstrate that miR-320c inhibits SERPINA1 expression 
      in a hepatic cell line and its levels in blood are associated with lung disease 
      in a cohort of patients with different AAT serum levels. These results suggest 
      that miR-320c can play a role in AAT regulation and could be a biomarker of 
      inflammatory processes in pulmonary diseases.
CI  - Copyright (c) 2020 SEPAR. Publicado por Elsevier Espana, S.L.U. All rights 
      reserved.
FAU - Matamala, Nerea
AU  - Matamala N
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain.
FAU - Lara, Beatriz
AU  - Lara B
AD  - Respiratory Medicine Department, Coventry University Hospital, Coventry, UK.
FAU - Gomez-Mariano, Gema
AU  - Gomez-Mariano G
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain.
FAU - Martinez, Selene
AU  - Martinez S
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain.
FAU - Vazquez-Dominguez, Irene
AU  - Vazquez-Dominguez I
AD  - Centro de Biologia Molecular Severo Ochoa (CBMSO), Consejo Superior de 
      Investigaciones Cientificas-Universidad Autonoma de Madrid (CSIC-UAM), Madrid, 
      Spain.
FAU - Otero-Sobrino, Alvaro
AU  - Otero-Sobrino A
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain.
FAU - Munoz-Callejas, Antonio
AU  - Munoz-Callejas A
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain.
FAU - Sanchez, Elena
AU  - Sanchez E
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain.
FAU - Esquinas, Cristina
AU  - Esquinas C
AD  - Registro Espanol de pacientes con deficit de alfa-1 antitripsina (REDAAT), 
      Fundacion Espanola de Pulmon, Respira, SEPAR, Barcelona, Spain; Servicio de 
      Neumologia, Hospital Universitari Vall d'Hebron/Vall d'Hebron Institut de Recerca 
      (VHIR), CIBER de Enfermedades Respiratorias (CIBERES), Barcelona, Spain.
FAU - Bustamante, Ana
AU  - Bustamante A
AD  - Servicio de Neumologia, Hospital de Sierrallana, Torrelavega, Cantabria, Spain.
FAU - Cadenas, Sergio
AU  - Cadenas S
AD  - Servicio de Neumologia, Hospital Clinico Universitario de Salamanca, Spain.
FAU - Curi, Sergio
AU  - Curi S
AD  - Complejo Hospitalario de Navarra, Pamplona, Spain.
FAU - Lazaro, Lourdes
AU  - Lazaro L
AD  - Servicio de Neumologia, Complejo Asistencial Universitario de Burgos, Spain.
FAU - Martinez, Maria Teresa
AU  - Martinez MT
AD  - Servicio de Neumologia, Hospital Universitario Doce de Octubre, Madrid, Spain.
FAU - Rodriguez, Esther
AU  - Rodriguez E
AD  - Servicio de Neumologia, Hospital Universitari Vall d'Hebron/Vall d'Hebron 
      Institut de Recerca (VHIR), CIBER de Enfermedades Respiratorias (CIBERES), 
      Barcelona, Spain.
FAU - Miravitlles, Marc
AU  - Miravitlles M
AD  - Servicio de Neumologia, Hospital Universitari Vall d'Hebron/Vall d'Hebron 
      Institut de Recerca (VHIR), CIBER de Enfermedades Respiratorias (CIBERES), 
      Barcelona, Spain.
FAU - Torres-Duran, Maria
AU  - Torres-Duran M
AD  - Servicio de Neumologia, Hospital Alvaro Cunqueiro, EOXI Vigo, Pneumovigo I+i, IIS 
      Galicia Sur, Spain.
FAU - Herrero, Ines
AU  - Herrero I
AD  - Hospital Universitario Miguel Servet, Zaragoza, Spain.
FAU - Michel, Francisco Javier
AU  - Michel FJ
AD  - Servicio de Neumologia, Hospital Universitario Donostia, Pais Vasco, Spain.
FAU - Castillo, Silvia
AU  - Castillo S
AD  - Unidad de Neumologia infantil y Fibrosis quistica, Hospital Clinico Universitario 
      de Valencia, Spain.
FAU - Hernandez-Perez, Jose M feminine
AU  - Hernandez-Perez JM
AD  - Servicio de Neumologia, Hospital General de la Palma, Canarias, Spain.
FAU - Blanco, Ignacio
AU  - Blanco I
AD  - Registro Espanol de pacientes con deficit de alfa-1 antitripsina (REDAAT), 
      Fundacion Espanola de Pulmon, Respira, SEPAR, Barcelona, Spain.
FAU - Casas, Francisco
AU  - Casas F
AD  - Servicio de Neumologia, Hospital Universitario San Cecilio, Granada, Spain.
FAU - Martinez-Delgado, Beatriz
AU  - Martinez-Delgado B
AD  - Molecular Genetics Unit, Instituto de Investigacion de Enfermedades Raras (IIER), 
      Instituto de Salud Carlos III (ISCIII), CIBER de Enfermedades Raras (CIBERER), 
      Madrid, Spain. Electronic address: bmartinezd@isciii.es.
LA  - eng
LA  - spa
PT  - Journal Article
DEP - 20200518
PL  - Spain
TA  - Arch Bronconeumol (Engl Ed)
JT  - Archivos de bronconeumologia
JID - 101777538
SB  - IM
OTO - NOTNLM
OT  - Alpha-1 antitrypsin deficiency
OT  - Deficit de alfa-1 antitripsina
OT  - Enfermedad pulmonar
OT  - Inflamacion
OT  - Inflammation
OT  - Lung disease
OT  - miR-320c
OT  - microARNs
OT  - microRNA
EDAT- 2020/05/23 06:00
MHDA- 2020/05/23 06:00
CRDT- 2020/05/23 06:00
PHST- 2019/08/29 00:00 [received]
PHST- 2020/03/11 00:00 [revised]
PHST- 2020/03/11 00:00 [accepted]
PHST- 2020/05/23 06:00 [entrez]
PHST- 2020/05/23 06:00 [pubmed]
PHST- 2020/05/23 06:00 [medline]
AID - S0300-2896(20)30084-3 [pii]
AID - 10.1016/j.arbres.2020.03.006 [doi]
PST - aheadofprint
SO  - Arch Bronconeumol (Engl Ed). 2020 May 18:S0300-2896(20)30084-3. doi: 
      10.1016/j.arbres.2020.03.006.